Deciphering the multicomponent synergy mechanisms of SiNiSan prescription on irritable bowel syndrome using a bioinformatics/network topology based strategy

Li, Bangjie; Rui, Junqian; Ding, Xuejian; Chen, Yifan; Yang, Xinghao

doi:10.1016/j.phymed.2019.152982

Cited by 22 publications

(15 citation statements)

References 88 publications

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“…The specific ratio among these components, also known as structural composition of multi‐components, may be the contributor to the better efficacy of CMM 25 . A large number of studies have found that the efficacy of TCM mainly depends on the synergy between the various components 26–28 . Based on the structural composition of multi‐components, we consider that there is a certain ratio among the ingredients.…”

Section: Resultsmentioning

confidence: 99%

The combination of HPLC and biological analysis to determine the quality markers and its structural composition of Eclipta prostrata L.

Wang¹,

Huang²,

Li³

et al. 2020

Phytochemical Analysis

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Introduction: The diversity and complexity of components are important reasons for the unstable efficacy and safety of Chinese materia medica (CMM) in quality control. The good and stable quality control may be related to the quality markers with structural composition of multi-components. Objective: In the present study, we take Eclipta prostrata L. as a representative example. The 11 samples were collected from the different areas in China, and the discrepancy in bioactivity and chemical composition of these samples were compared. DOX (doxorubicin hydrochloride)-induced ICR mice were established for in vivo nephrotic syndrome experiments. The biochemical indicators including 24-h urine protein, triglyceride (TG), etc. were measured and the pathological change of kidney tissue was observed. MPC-5 cells damage model was induced to compare the difference of these samples in bio-activity. High-performance liquid chromatography (HPLC) method for 11 EEP (extract of Eclipta prostrata L.) samples were performed to analyse the content of the quality markers. Results: In vivo experiments, EEP could mitigate the content or activity of urine protein, TG, etc. compared with the positive group (TG content was 2.53 ± 0.39 mmol/L, urinary protein quantification on 35th day was 16.79 ± 2.32 mg). In vitro experiments, CCE (coumestans component of Ecliptae) and EEP had equivalent effects on biochemical indicators such as cell viability, etc. According to the HPLC analysis, the content of wedelolactone was 45.88% and demethylwedelolactone was 23.74% in CCE. Conclusion: The CCE with a ratio of 2:1 can be considered as a quality marker of Eclipta prostrata L.. This research may provide a perspective for quality control of CMM.

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Section: Resultsmentioning

confidence: 99%

The combination of HPLC and biological analysis to determine the quality markers and its structural composition of Eclipta prostrata L.

Wang¹,

Huang²,

Li³

et al. 2020

Phytochemical Analysis

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“…Many studies demonstrated the multicomponent, multitarget, and multichannel characteristics of TCM [35–38]. It acquires special treatment efficacy by acting on the biological network of body systems.…”

Section: Discussionmentioning

confidence: 99%

The Antitumor Potential of Extract of the Oak Bracket Medicinal Mushroom Inonotus baumii in SMMC-7721 Tumor Cells

Yang

2019

Evidence-Based Complementary and Alternative Medicine

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Inonotus baumii, a traditional medicinal mushroom, has been historically used in China and other countries of East Asia for the treatment of various diseases. The aim of this study is to investigate the antitumor activity of the extract of I. baumii (EIB) against hepatocellular carcinoma and the possible mechanism involved. The MTT assay was used to evaluate the proliferative activity of SMMC-7721 cells treated with EIB. Hoechst 33258 and JC-1 staining were used to determine nuclear morphological changes and mitochondrial membrane potential, respectively. Flow cytometry analysis indicated that EIB blocked the cell cycle at the S phase and induced significant apoptosis. EIB increased the protein expression of Bax, cytochrome c, cleaved caspase-3, and decreased Bcl-2 in SMMC-7721. Moreover, EIB induced autophagy, indicated by the increase of autophagy-related protein expression of LC3-II and decrease of p62, and the AMPK/mTOR/ULK1 pathway was involved in the autophagic cell death. In vivo, EIB was found to strongly inhibit the growth of tumors in BALB/c nude mice. Our results indicated that I. baumii might be a potential natural therapeutic agent for liver cancer, as it could induce apoptosis and autophagy in HCC cells.

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“…In clinical medication, MDD and NAFLD can be treated with Sini powder (a prescription in TCM), which has been verified by relevant experiments and has achieved good therapeutic effects (239,240). These outcomes may be highly related to its multi-target and multi-path characteristics (241)(242)(243). However, due to the complexities of TCM compounds and the possibility of chemical composition changes after processing (244), studies on Chinese medicine are challenging.…”

Section: Discussionmentioning

confidence: 99%

Interaction Mechanisms Between Major Depressive Disorder and Non-alcoholic Fatty Liver Disease

Shao

Jiang

et al. 2021

Front. Psychiatry

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Major depressive disorder (MDD), which is highly associated with non-alcoholic fatty liver disease (NAFLD), has complex pathogenic mechanisms. However, a limited number of studies have evaluated the mutual pathomechanisms involved in MDD and NAFLD development. Chronic stress-mediated elevations in glucocorticoid (GC) levels play an important role in the development of MDD-related NAFLD. Elevated GC levels can induce the release of inflammatory factors and changes in gut permeability. Elevated levels of inflammatory factors activate the hypothalamic–pituitary–adrenal (HPA) axis, which further increases the release of GC. At the same time, changes in gut permeability promote the release of inflammatory factors, which results in a vicious circle among the three, causing disease outbreaks. Even though the specific role of the thyroid hormone (TH) in this pathogenesis has not been fully established, it is highly correlated with MDD and NAFLD. Therefore, changing lifestyles and reducing psychological stress levels are necessary measures for preventing MDD-related NAFLD. Among them, GC inhibitors and receptor antagonists may be key in the alleviation of early and mid-term disease progression. However, combination medications may be important in late-stage diseases, but they are associated with various side effects. Traditional Chinese medicines have been shown to be potential therapeutic alternatives for such complex diseases.

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Deciphering the multicomponent synergy mechanisms of SiNiSan prescription on irritable bowel syndrome using a bioinformatics/network topology based strategy

Cited by 22 publications

References 88 publications

The combination of HPLC and biological analysis to determine the quality markers and its structural composition of Eclipta prostrata L.

The combination of HPLC and biological analysis to determine the quality markers and its structural composition of Eclipta prostrata L.

The Antitumor Potential of Extract of the Oak Bracket Medicinal Mushroom Inonotus baumii in SMMC-7721 Tumor Cells

Interaction Mechanisms Between Major Depressive Disorder and Non-alcoholic Fatty Liver Disease

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