Deciphering the metabolic signatures of Trigonella microgreens as a function of photoperiod and temperature using targeted compound analysis and non-targeted UHPLC-QTOF-IMS based approach

Kumar, Arun; Singh, Narpinder; Joshi, Robin

doi:10.1016/j.foodres.2023.113834

Food Research International

2024

DOI: 10.1016/j.foodres.2023.113834

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Deciphering the metabolic signatures of Trigonella microgreens as a function of photoperiod and temperature using targeted compound analysis and non-targeted UHPLC-QTOF-IMS based approach

Arun Kumar,

Narpinder Singh,

Robin Joshi

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2024

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Cited by 2 publications

References 48 publications

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Evaluation of antioxidant and ACE inhibitory peptides in rice bran protein hydrolysate using non‐targeted UHPLC‐QTOF‐IMS profiling, MALDI‐TOF‐TOF‐MS/MS, and molecular docking study

Kumar,

Singh,

Joshi

2024

Int J of Food Sci Tech

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SummaryIn the present study, rice bran protein (RBP) from the PR1121 variety was hydrolyzed using trypsin and subjected to non‐targeted IMS profiling to identify antioxidant and antihypertensive amino acids in the resulting RBP hydrolysates. The hydrolysate was then separated into three fractions using ultrafiltration, and each fraction was tested for antioxidant and ACE inhibitory activities. The ACE activity was assessed using the tripeptide hippuryl‐histidyl‐leucine (HHL) as a model peptide via HPLC‐DAD. The fraction with the molecular weight ( >3000 Da) displayed the strongest antioxidant and ACE inhibitory activity. This fraction was further divided into five fractions using gel sephadex G‐25, and the greatest levels of antioxidant and ACE inhibitory activity were found in fraction F3B. Fraction F3B was then fractionated using HPLC, and the fraction with ACE inhibitory activity (IC50 of 43 mg/mL) was analyzed using MALDI‐TOF‐TOF mass spectrometry to determine its exact molecular mass and amino acid sequence. The amino acid sequence FMKSK (phe‐met‐lys‐ser‐lys) with a Mw of 655.376 Da was detected, and the molecular docking investigation revealed that FMKSK suppresses ACE by forming strong hydrogen bonds with the active pockets of human ACE. These findings suggest that rice bran contains bioactive peptides with antioxidant and ACE inhibitory effects, making it a promising raw material for the production of beneficial products.

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Evaluation of antioxidant and ACE inhibitory peptides in rice bran protein hydrolysate using non‐targeted UHPLC‐QTOF‐IMS profiling, MALDI‐TOF‐TOF‐MS/MS, and molecular docking study

Kumar,

Singh,

Joshi

2024

Int J of Food Sci Tech

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Comprehensive analysis of targeted phenolic, sugar, and amino acid variation in 10‐day‐old wheatgrass in response to temperature and photoperiod

Kumar,

Singh

2024

Int J of Food Sci Tech

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SummaryThe present study investigated the phenolic profile, sugar composition and amino acid profile of lyophilised 10‐day‐old wheatgrass cultivated under varying temperature and photoperiod conditions. The targeted phenolic profile showed the abundant presence of chlorogenic, caffeic, ferulic and p‐coumaric acid. The phenolic content varied with temperature and photoperiod conditions. The comprehensive sugar profile indicated the variation in the concentration of glucose with different growing conditions altered carbohydrate metabolism in wheatgrass. Similarly, the concentration of amino acids also varied with growing conditions. Notably, the concentration of proline, arginine and GABA in wheatgrass from all wheat varieties increased more prominently in photoperiod of 22 h and growth temperature of 22 °C in light and 17 °C in dark as a response of defence mechanism.

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Deciphering the metabolic signatures of Trigonella microgreens as a function of photoperiod and temperature using targeted compound analysis and non-targeted UHPLC-QTOF-IMS based approach

Cited by 2 publications

References 48 publications

Evaluation of antioxidant and ACE inhibitory peptides in rice bran protein hydrolysate using non‐targeted UHPLC‐QTOF‐IMS profiling, MALDI‐TOF‐TOF‐MS/MS, and molecular docking study

Evaluation of antioxidant and ACE inhibitory peptides in rice bran protein hydrolysate using non‐targeted UHPLC‐QTOF‐IMS profiling, MALDI‐TOF‐TOF‐MS/MS, and molecular docking study

Comprehensive analysis of targeted phenolic, sugar, and amino acid variation in 10‐day‐old wheatgrass in response to temperature and photoperiod

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