Deciphering the interactions between chlorambucil and calf thymus DNA: A multi-spectroscopic and molecular docking study

Rehman, Sufia; Sarwar, Tarique; Ishqi, Hassan Mubarak; Husain, Mohammed Amir; Hasan, Ziaul; Tabish, Mohammad

doi:10.1016/j.abb.2014.12.013

Cited by 102 publications

(43 citation statements)

References 31 publications

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“…Groove binders do not change the relative viscosity of DNA (Fig. 4D) since they do not alter the axial length of DNA upon binding [41]. However, a slight increase in the relative viscosity may also be considered as an indication of groove binding.…”

Section: Viscosity Measurementsmentioning

confidence: 92%

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Studying non-covalent drug–DNA interactions

Rehman

Sarwar

Husain

et al. 2015

Archives of Biochemistry and Biophysics

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Section: Viscosity Measurementsmentioning

confidence: 92%

“…The extent of fluorescence quenching of DNA-EB system can be used to determine the extent of intercalation between the molecule and DNA [37,40]. Groove binding and surface binding molecules show no effect on the DNA-EB fluorescence intensity [41,42].…”

Section: Competitive Displacement Assaysmentioning

confidence: 99%

Studying non-covalent drug–DNA interactions

Rehman

Sarwar

Husain

et al. 2015

Archives of Biochemistry and Biophysics

Self Cite

308

160

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“…Thus, the binding mode between the β-carboline alkaloids with ctDNA was proposed according to three strategies: (i) evaluation of the effect of KI quenching, (ii) competition with ethidium bromide, and (iii) the profile of the DNA thermal denaturation. 42,43 The iodide ion is capable of promoting the dynamic quenching of fluorescent molecules in solution. However, the molecule must be accessible for the interaction that occurs with the iodide ion.…”

Section: Evaluation Of Ctdna-alkaloid Binding Modementioning

confidence: 99%

“…If the K SV values in the presence of ctDNA have been larger, it would be an indication that the iodide would have access to the compound, and thus, the most probable binding mode would not be by intercalation. 16,42 To confirm that compounds evaluated interact by intercalation, a competition assay with ethidium bromide was performed. Ethidium bromide in free form presents low fluorescence emission, but when it intercalates with DNA occurs a considerable increase in fluorescence intensity, as shown in Figure S2a.…”

Section: Evaluation Of Ctdna-alkaloid Binding Modementioning

confidence: 99%

Interaction of β-Carbolines with DNA: Spectroscopic Studies, Correlation with Biological Activity and Molecular Docking

Silva

Savariz

Silva-Júnior

et al. 2016

Journal of the Brazilian Chemical Society

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Quantitative interaction of twelve β-carboline derivatives with calf thymus deoxyribonucleic acid (ctDNA) using spectroscopic techniques was evaluated. The values of the binding constants (K b ) obtained for the complexes formed with the ctDNA ranged from 3.30 × 10 2 to 1.82 × 10 6 mol L −1 , being the β-carbolines with the N,N-dimethylaminophenyl group at position 1 the ones which presented the highest K b values. The binding mode between the β-carbolines evaluated and ctDNA was proposed from the KI assay, competition with ethidium bromide, and DNA thermal denaturation profile (T m ), where it was possible to infer that the evaluated alkaloids interact with ctDNA preferably via intercalation. Additionally, the correlation of K b values obtained with the IC 50 of seven human cancer cell lines was carried out. From this study, it was possible to observe a linear relation among most of the evaluated derivatives, obtaining r 2 values from 0.5360 to 0.9600. In addition, in silico molecular docking was performed to corroborate the experimental results.

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“…specific hepatocyte binding. 12 While the importance of this region in HBV binding was recognized early on, the corresponding receptor molecule on the hepatocyte membrane has long been a mystery, and many candidate molecules have been proposed, including asiaglycoprotein receptor, transferrin receptor, and IL-6 receptor (reviewed in Glebe and Urban 13 and Rehman et al 14 ). A recent study also proposed ferritin light chain and squamous cell carcinoma antigen 1 as co-receptors for HBV entry.…”

Section: Introductionmentioning

confidence: 99%

Early events in hepatitis B virus infection: From the cell surface to the nucleus

Hayes

Zhang

Makokha

et al. 2016

J of Gastro and Hepatol

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While most adults are able to clear acute hepatitis B virus (HBV) infection, chronic HBV infection is recalcitrant to current therapy because of the persistence of covalently closed circular DNA in the nucleus. Complete clearance of the virus in these patients is rare, and long-term therapy with interferon and/or nucleoside analogues may be required in an attempt to suppress viral replication and prevent progressive liver damage. The difficulty of establishing HBV infection in cell culture and experimental organisms has hindered efforts to elucidate details of the HBV life cycle, but it has also revealed the importance of the cellular microenvironment required for HBV binding and entry. Recent studies have demonstrated an essential role of sodium-taurocholate cotransporting polypeptide as a functional receptor in HBV infection, which has facilitated the development of novel infection systems and opened the way for more detailed understanding of the early steps of HBV infection as well as a potential new therapeutic target. However, many gaps remain in understanding of how HBV recognizes and attaches to hepatocytes prior to binding to sodium-taurocholate cotransporting polypeptide, as well as events that are triggered after binding, including entry into the cell, intracellular transport, and passage through the nuclear pore complex. This review summarizes current knowledge of the initial stages of HBV infection leading to the establishment of covalently closed circular DNA in the nucleus.

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Deciphering the interactions between chlorambucil and calf thymus DNA: A multi-spectroscopic and molecular docking study

Cited by 102 publications

References 31 publications

Studying non-covalent drug–DNA interactions

Studying non-covalent drug–DNA interactions

Interaction of β-Carbolines with DNA: Spectroscopic Studies, Correlation with Biological Activity and Molecular Docking

Early events in hepatitis B virus infection: From the cell surface to the nucleus

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