Deciphering the genome of Simplicillium aogashimaense to understand its mechanisms against the wheat powdery mildew fungus Blumeria graminis f. sp. tritici

Zhu, Mo; Duan, Xiao; Cai, Pengkun; Li, Yongfang; Qiu, Zongbo

doi:10.1186/s42483-022-00121-5

Cited by 17 publications

(10 citation statements)

References 54 publications

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“…First-Strand cDNA was synthesized from 2 µg total RNA of flash-frozen fresh roots according to the directions of the reverse transcription kit (TaKaRa, Dalian, China). RT-qPCR was performed as previously described (Zhu et al, 2022c). Each reaction had three biological replicates and tubulin (DQ435660.1) was used as a reference gene to normalize the expression of the candidate genes using the 2 − Ct method (Livak and Schmittgen, 2001).…”

Section: Rt-qpcr Validation and Expression Analysismentioning

confidence: 99%

Jasmonic acid pretreatment improves salt tolerance of wheat by regulating hormones biosynthesis and antioxidant capacity

Zhu

Liu²,

Cai³

et al. 2022

Front. Plant Sci.

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Salt stress is a severe environmental factor that detrimentally affects wheat growth and production worldwide. Previous studies illustrate that exogenous jasmonic acid (JA) significantly improved salt tolerance in plants. However, little is known about the underlying molecular mechanisms of JA induced physiochemical changes in wheat seedlings under salt stress conditions. In this study, biophysiochemical and transcriptome analysis was conducted to explore the mechanisms of exogenous JA induced salt tolerance in wheat. Exogenous JA increased salt tolerance of wheat seedlings by alleviating membrane lipid oxidation, improving root morphology, enhancing the contents of ABA, JA and SA and increasing relative water content. In the RNA-seq profiles, we identified a total of 54,263 unigenes and 1,407 unigenes showed differentially expressed patterns in JA pretreated wheat seedlings exposed to salt stress comparing to those with salt stress alone. Subsequently, gene ontology (GO) and KEGG pathway enrichment analysis characterized that DEGs involved in linoleic acid metabolism and plant hormone signal transduction pathways were up-regulated predominantly in JA pretreated wheat seedlings exposed to salt stress. We noticed that genes that involved in antioxidative defense system and that encoding transcription factors were mainly up- or down-regulated. Moreover, SOD, POD, CAT and APX activities were increased in JA pretreated wheat seedlings exposed to salt stress, which is in accordance with the transcript profiles of the relevant genes. Taken together, our results demonstrate that the genes and enzymes involved in physiological and biochemical processes of antioxidant system, plant hormones and transcriptional regulation contributed to JA-mediated enhancement of salt tolerance in wheat. These findings will facilitate the elucidation of the potential molecular mechanisms associated with JA-dependent amelioration of salt stress in wheat and lay theoretical foundations for future studies concerning the improvement of plant tolerance to abiotic environmental stresses.

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Section: Rt-qpcr Validation and Expression Analysismentioning

confidence: 99%

Jasmonic acid pretreatment improves salt tolerance of wheat by regulating hormones biosynthesis and antioxidant capacity

Zhu

Liu²,

Cai³

et al. 2022

Front. Plant Sci.

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“…The genome-assisted approach was used to determine the capability of producing antifungal metabolites by the JC-1 strain [ 17 ]. Genomic DNA was extracted from the mycelial masses of JC-1 using the CTAB method [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

“…tianjinensis , S. lepidopterorum , S. neolepidopterorum , S. niveum , S. scarabaeoidea , S. subtropicum and S. sympodiophorum [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. These Simplicillium species have diversified ecological niches, some live with fungi as mycoparasites [ 1 , 12 , 13 , 14 , 15 , 16 , 17 ], some live with insects as entomolopathogens [ 2 , 3 , 9 , 18 , 19 ], some live with plants as endophytes or pathogens [ 20 , 21 , 22 ], and some live as saprophytes in fresh water [ 7 ], in soil [ 8 ], on animal faeces [ 11 ], and even in the deep sea [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies showed several species of Simplicillium with the mycoparasitic lifestyle can potentially be used for the control of plant fungal diseases [ 15 , 17 , 19 , 24 , 25 ]. Choi et al (2008) found that S. lamellicola BCP (= Acremonium strictum BCP) is a mycoparasite of the gray mold fungus Botrytis cinerea [ 26 ], and it has been developed as a biofungicide for control of gray mold in tomato and ginseng [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Zhu et al (2022) reported that S. aogashimaense is a mycoparasite of the powdery mildew fungus Blumeria graminis f. sp. tritici ( Bgt ), and it had a high efficacy in the suppression of powdery mildew through impairing colony formation by Bgt on wheat leaves [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Antifungal Activity and Biocontrol Potential of Simplicillium lamellicola JC-1 against Multiple Fungal Pathogens of Oilseed Rape

Luo

et al. 2022

JoF

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A fungal strain (JC-1) of Simplicillium was isolated from a pod of oilseed rape (Brassica napus) infested with the blackleg pathogen Leptosphaeria biglobosa. This study was done to clarify its taxonomic identity using morphological and molecular approaches, to characterize its antifungal activity through bioassays and genome-based identification of antifungal metabolites, and to determine its efficacy in inducing systemic resistance (ISR) in oilseed rape. The results showed that JC-1 belongs to Simplicillium lamellicola. It displayed a strong antagonistic relationship with L. biglobosa, Botrytis cinerea (gray mold) and Sclerotinia sclerotiorum (stem rot). The cultural filtrates of JC-1 showed a high efficacy in suppressing infection by S. sclerotiorum on detached leaves of oilseed rape. Genome analysis indicated that JC-1 has the capability of producing multiple antifungal metabolites, including aureobasidin A1, squalestatin S1 and verlamelin. Inoculation of JC-1 on seeds of oilseed rape caused a suppressive effect on infection by L. biglobosa on the cotyledons of the resulting seedlings, suggesting that JC-1 can trigger ISR. Endophytic growth, accumulation of anthocyanins, up-regulated expression of CHI (for chalcone isomerase) and PR1 (for pathogenesis-related protein 1), and down-regulated expression of NECD3 (for 9-cis-epoxycarotenoid dioxygenase) were detected to be associated with the ISR. This study provided new insights into the biocontrol potential and modes of action of S. lamellicola.

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Abundance and diversity of fungal endophytes isolated from monk fruit (Siraitia grosvenorii) grown in a Canadian research greenhouse

Ma,

Elmhirst,

Darvish

et al. 2024

Plant Enviro Interactions

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Monk fruit (Siraitia grosvenorii) is an herbaceous perennial vine of the Cucurbitaceae family cultivated commercially mainly in southern China. There is very little information available about the fungal endophytes in monk fruit. In this study, monk fruit plants were grown from seeds in a research greenhouse at Kwantlen Polytechnic University in British Columbia, Canada to explore the abundance and diversity of their fungal endophytes. Fungal endophytes were isolated from seeds, seedlings, mature monk fruit plants, and fruits, and cultured on potato dextrose agar and water agar media. Isolates were identified by microscopic examination and BLAST comparison of ITS sequences to published sequences in GenBank. At least 150 species of fungal endophytes representing 60 genera and 20 orders were recovered from monk fruit tissues. Non‐metric multidimensional scaling (NMDS) was carried out to explore the similarity of fungal communities among roots, stems, leaves, flowers, fruits, and seeds based on fungal orders. Our study showed that monk fruit plants are a rich source of fungal endophytes with the greatest abundance and diversity in leaves. This work has deepened our understanding of the intricate interactions between plants and fungi that sustain ecosystems and underpin plant health and resilience.

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Deciphering the genome of Simplicillium aogashimaense to understand its mechanisms against the wheat powdery mildew fungus Blumeria graminis f. sp. tritici

Cited by 17 publications

References 54 publications

Jasmonic acid pretreatment improves salt tolerance of wheat by regulating hormones biosynthesis and antioxidant capacity

Jasmonic acid pretreatment improves salt tolerance of wheat by regulating hormones biosynthesis and antioxidant capacity

Antifungal Activity and Biocontrol Potential of Simplicillium lamellicola JC-1 against Multiple Fungal Pathogens of Oilseed Rape

Abundance and diversity of fungal endophytes isolated from monk fruit (Siraitia grosvenorii) grown in a Canadian research greenhouse

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