“…SEM images were used to evaluate the preservation of the microstructures of the secondary layer, the shell microstructures are considered to be well preserved when smooth fibrous surfaces of secondary layer occur, similarly to those observed in living specimens (Brand et al, 2003;Suan et al, 2008a). Diagenetically-altered structures of the secondary layer of brachiopod shells are identified by the poor individualization of calcite fibres (Suan et al, 2008a;Giraud et al, 2016). In specimens showing a good preservation of the calcite fibres of the secondary layer under SEM, the primary character of the stable carbon and oxygen isotope composition of the brachiopod shells was tested using the concentrations of strontium and manganese, which are known to be sensitive to diagenetic alteration (Brand and Veizer, 1980;Brand et al, 2003).…”