Deciphering miRNA–Target Relationships to Understand miRNA-Mediated Carcinogenesis

Navarro, Alfons

doi:10.3390/cancers13102415

Cited by 3 publications

(1 citation statement)

References 22 publications

(21 reference statements)

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“…Most of the miRNA expressions are highly tissue-speci c and cell-speci c, so they can be used in disease diagnosis and treatment, which has become a new research focus [27,28,29,30]. The main function of miRNA is the post-transcriptional regulation of protein-coding genes, which binds to the target mRNA and inhibits its translation in a sequence-dependent manner [31,32,33]. Our group screened a series of glomerulonephritis-related miRNA by consulting the literature, and further predicted through bioinformatics prediction and analysis that there may be a complementary sequence between miR-339-5p and the central gene Syk of glomerulonephritis veri ed by our group [34], which plays an important role in cell proliferation, apoptosis, in ammation and so on [35].…”

Section: Syk Downregulation Reversed the Effects Of Mir-339-5p On Hbzy-1 Cellsmentioning

confidence: 99%

MicroRNA-339-5p inhibits lipopolysaccharide-induced rat mesangial cells by regulating the Syk/Ras/c-Fos pathway

Gao

shi

Jiang

et al. 2022

Preprint

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Purpose: Chronic glomerulonephritis (CGN) is a disease that occurs in the glomeruli. The mechanism of CGN is thought to be involved in a range of inflammatory responses. MicroRNA-339-5p (miR-339-5p) has been reported to be involved in inflammatory responses in many diseases. However, the role of miR -339-5p in CGN remains unclear. The purpose of this study was to investigate the role of miR-339-5p in lipopolysaccharide (LPS) -induced nephritis injury in vitro. Methods: The RNA expression of miR-339-5p and Syk/Ras/c-Fos pathway was detected by qRT-PCR, the protein expression and localization of Syk/Ras/c-Fos pathway was detected by western blot and immunofluorescence (IF), and the targeted binding of miR-339-5p to Syk was detected by double luciferase. Cell viability and cell cycle were detected by cell counting kit-8 (CCK-8) and flow cytometry. The concentrations of inflammatory cytokines IL-1β, IL-10, IL-6 and TNF-α were detected by enzyme linked immunosorbent assay (ELISA). Results: LPS increased HBZY-1 cell viability, decreased G2 phase, promoted cell proliferation and inflammatory cytokine release. Overexpression of miR-339-5p can inhibit HBZY-1 cell viability, decreased the expression of Syk/Ras/c-Fos signaling pathway, down-regulate the expression level of inflammatory cytokines, increase G2 phase, and inhibit cell proliferation.Conclusion: miR-339-5p inhibits the proliferation and inflammation of rat mesangial cell through Syk/Ras/c-Fos signal pathway.

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Section: Syk Downregulation Reversed the Effects Of Mir-339-5p On Hbzy-1 Cellsmentioning

confidence: 99%

MicroRNA-339-5p inhibits lipopolysaccharide-induced rat mesangial cells by regulating the Syk/Ras/c-Fos pathway

Gao

shi

Jiang

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

MicroRNA-339-5p inhibits lipopolysaccharide-induced rat mesangial cells by regulating the Syk/Ras/c-Fos pathway

Gao

Shi

Jiang

et al. 2022

Naunyn-Schmiedeberg's Arch Pharmacol

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The microRNAs in the antennae of Apolygus lucorum (Hemiptera: Miridae): Expression properties and targets prediction

Khashaveh

Shan

et al. 2022

Genomics

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Deciphering miRNA–Target Relationships to Understand miRNA-Mediated Carcinogenesis

Abstract: We now accept that the non-coding part of the genome is essential for fine-tuning most cellular functions, and that its deregulation drives carcinogenesis [...]

Cited by 3 publications

References 22 publications

MicroRNA-339-5p inhibits lipopolysaccharide-induced rat mesangial cells by regulating the Syk/Ras/c-Fos pathway

MicroRNA-339-5p inhibits lipopolysaccharide-induced rat mesangial cells by regulating the Syk/Ras/c-Fos pathway

MicroRNA-339-5p inhibits lipopolysaccharide-induced rat mesangial cells by regulating the Syk/Ras/c-Fos pathway

The microRNAs in the antennae of Apolygus lucorum (Hemiptera: Miridae): Expression properties and targets prediction

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