Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells

Monetti, Emanuela; Kadono, Takashi; Tran, Daniel; Azzarello, Elisa; Arbelet-Bonnin, Delphine; Biligui, Bernadette; Briand, Joël; Kawano, Tomonori; Mancuso, Stefano; Bouteau, François

doi:10.1093/jxb/ert460

Cited by 46 publications

(45 citation statements)

References 62 publications

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“…In response to DON we recorded a time-dependent swelling and depolarization of the mitochondrion clearly indicating mitochondrial dysfunction. This mitochondrial swelling, decrease in m , and the cell death were largely attenuated in the presence of CsA, a blocker of PTP [36,37,57] as already observed in different environmentally and developmentally regulated cell death [5,17]. It is noteworthy that CsA was also efficient in decreasing DON-induced ROS generation (Supplemental Fig.…”

Section: Discussionsupporting

confidence: 73%

“…Nicotiana tabacum BY2 cell suspensions were grown in Murashige and Skoog medium (MS medium) at pH 5.8 [17]. They were maintained at 22 ± 2 • C in darkness with continuous shaking at 120 rpm.…”

Section: Plant Cell Culture Conditionsmentioning

confidence: 99%

“…Reactive oxygen species (ROS) release in the medium was quantified by measuring the chemiluminescence of luminol reacting with ROS [17]. Briefly, 10 ml of the N. tabacum BY2 cell suspension was inoculated with 50 g ml −1 of DON alone or with the appropriate chemical effectors.…”

Section: Measurement Of Reactive Oxygen Species Productionmentioning

confidence: 99%

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Early events induced by the toxin deoxynivalenol lead to programmed cell death in Nicotiana tabacum cells

et al. 2015

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Deoxynivalenol (DON) is a mycotoxin affecting animals and plants. This toxin synthesized by Fusarium culmorum and Fusarium graminearum is currently believed to play a decisive role in the fungal phytopathogenesis as a virulence factor. Using cultured cells of Nicotiana tabacum BY2, we showed that DON-induced programmed cell death (PCD) could require transcription and translation processes, in contrast to what was observed in animal cells. DON could induce different cross-linked pathways involving (i) reactive oxygen species (ROS) generation linked, at least partly, to a mitochondrial dysfunction and a transcriptional down-regulation of the alternative oxidase (Aox1) gene and (ii) regulation of ion channel activities participating in cell shrinkage, to achieve PCD.

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Section: Discussionsupporting

confidence: 73%

Section: Plant Cell Culture Conditionsmentioning

confidence: 99%

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Early events induced by the toxin deoxynivalenol lead to programmed cell death in Nicotiana tabacum cells

et al. 2015

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“…Experimental systems to understand the effects of drought have incorporated cell-impermeable high molecular weight polyethylene glycol (PEG;Lagerwerff et al, 1961;Verslues et al, 1998). The use of PEG showed that drought stress response could be separated into components of high osmotic pressure effects and of high salt toxicity effects, with each exhibiting distinct transcriptome profiles (Patade et al, 2012;Monetti et al, 2014). Indeed, PEG-generated osmotic stress was used to select for novel drought-resistant rice genotypes in embryogenic calli.…”

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confidence: 99%

Singlet Oxygen Plays an Essential Role in the Root’s Response to Osmotic Stress

Chen

Fluhr

2018

Plant Physiol.

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As SOSG fluorescence indicated the presence of singlet oxygen both before and after cell death, it is important to consider whether the appearance of singlet oxygen is an essential component of PEG-induced . Relationship between integrity of the quiescent center and root elongation. A, Fluorescence resulting from SCR::GFP expression in the endodermis and quiescent center. Roots were exposed to 30% PEG for 1 to 8 h and counterstained with propidium iodide (PI). The median plane was imaged where opaque regions in PI staining indicate cell death. Arrows indicate the quiescent center; scale bars, 100 μm. B, Root elongation rate following exposure to PEG. Student's t test, alpha = 0.05; difference in letters signifies a P < 0.05 significant difference; error bars are se, n ≥ 13. stress response or a symptom of cell death. In the former possibility, singlet oxygen would play a direct role in the PEG responses, and specific scavengers of singlet oxygen should mitigate its action. To test this, His, a membrane-permeable specific scavenger of singlet oxygen, was employed. His rapidly forms specific endoperoxides in the presence of singlet oxygen. It is a weaker scavenger of hydroxyl radicals and hydrogen peroxide and does not scavenge superoxide (Matheson et al., 1975; Cai et al., 1995; Ishibashi et al., 1996). The specificity of His as a scavenger for singlet oxygen as opposed to the other ROS is shown in Supplemental Figure S6. As indicated in Figure 6, A and B, His readily mitigates the appearance of singlet oxygen as measured by SOSG. Significantly, the amount of cell death was reduced by 50% ( Fig. 6B) and, as shown in Figure 6C, the presence of His alleviated the repression of the rate of root elongation by PEG. In all, the results lend support for an active role of singlet oxygen in the response to osmotic stress.The light-independent origin of singlet oxygen is unclear. SHAM is a nonspecific inhibitor of mitochondrial Composite of multiple confocal images of ROS accumulation in root tips exposed to 30% PEG for different durations. A, Composite of confocal images that were collated for each specific signal (ROS probes and propidium iodide stain). Multiple image acquisitions for each time point were aligned manually by rotation and overlaid to create the average composite image using Matlab script. The Look Up Table was set to "Fire" and scaled to maximum as white and minimum as blue. Shown are 1 O 2 , measured using the SOSG probe; NO, measured using the DAF-FM-AM probe; O − 2, measured using the BesSo-AM probe; H 2 O 2 , measured using the Bes-H 2 O 2 -AC probe, and cell permeability, measured using staining by propidium iodide. B, Schematic representation of the fluorescence shown in A. Scale bar, 100 µm. Figure 5. Singlet oxygen-and hydrogen peroxide-specific gene expression in roots. A, Transcript levels were measured by RT-qPCR analysis at 0, 1, 2, and 3 h after treatment with 30% PEG. B, Specificity of gene expression. The transcript level fold change in roots; no treatment (Control); treatment for 2 h in ...

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“…Although involvement of O 2 •À produced via the function of NADPH oxidase is implicated in PCD pathway under salt and osmotic stress, signals activated by ionic salt and nonionic osmotic stress inducer (i.e., sorbitol) did not show extensive overlap (Monetti et al 2014;Petrov et al 2015), indicating the specificity of the mechanism regulating PCD under these stresses. Specific feature of salt-induced PCD is characterized by ion disequilibrium that may be due to invasion of Na + into the cytosol accompanied by a decrease in K + .…”

Section: Programmed Cell Death Regulated By Ros Under Abiotic Stressmentioning

confidence: 93%

ROS as Key Players of Abiotic Stress Responses in Plants

Suzuki

2015

Reactive Oxygen Species and Oxidative Damage in Plants Under Stress

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Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells

Cited by 46 publications

References 62 publications

Early events induced by the toxin deoxynivalenol lead to programmed cell death in Nicotiana tabacum cells

Early events induced by the toxin deoxynivalenol lead to programmed cell death in Nicotiana tabacum cells

Singlet Oxygen Plays an Essential Role in the Root’s Response to Osmotic Stress

ROS as Key Players of Abiotic Stress Responses in Plants

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