Deciduous and Permanent Dental Pulp Mesenchymal Cells Acquire Hepatic Morphologic and Functional Features In Vitro

Ishkitiev, Nikolay; Yaegaki, Ken; Calenic, Bogdan; Nakahara, Taka; Ishikawa, Hiroshi; Mitiev, Vanyo; Haapasalo, Markus

doi:10.1016/j.joen.2009.12.022

Cited by 116 publications

(117 citation statements)

References 22 publications

(15 reference statements)

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“…3 Dental pulp stem cells (DPSCs) have been differentiated 4 into odontoblasts; 5,6 osteoblasts; 1 adipocytes, chondrocytes, and osteocytes; 7,8 nerve cells; 8 and hepatocytes. 9 Furthermore, the combined use of tissue engineering and DPSCs has enabled the regeneration of a dentin-pulp complex, 10 the generation of functional roots, 11 and even the production of a complete tooth. 12 Dental pulp stem cells have survived and proliferated in a self-assembling peptide hydrogel that could conceptually be used in regenerative endodontics, allowing stem cell therapy inside the root canal.…”

Section: Introductionmentioning

confidence: 99%

Culture of human dental pulp cells at variable times post-tooth extraction

Araújo

Oliveira²,

et al. 2018

Braz. oral res.

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The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.

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Section: Introductionmentioning

confidence: 99%

Culture of human dental pulp cells at variable times post-tooth extraction

Araújo

Oliveira²,

et al. 2018

Braz. oral res.

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“…Chivu et al showed incomplete hepatogenic differentiation of human BM-MSCs when HGF, nicotinamide and dexamethasone were separately added in differentiation medium. Conversely, hepatocytelike cells features were observed after combination of all these factors in the culture [48].…”

Section: Induction By Soluble Growth Factors or Cytokines-defined Mediummentioning

confidence: 93%

Mesenchymal Stem Cells: Current Clinical Applications and Therapeutic Potential in Liver Diseases

Prasajak¹

2014

J Bone Marrow Res

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Mesenchymal stem cells (MSCs) are one of valuable candidates for cell-based therapy which trend to be safe, feasible and promising in the treatment of several diseases. Since, MSCs possess unique biological characteristics which make them variable applying in clinic. These include self-renewal capacity, multi-lineages differentiation potential, homing and migration ability, immunomodulatory properties and paracrine secretion activity. These beneficial advantages lead to increase possibility of using MSCs in several implications such as regenerative medicine, tissue engineering, cells-based therapy and other clinical applications. At present, many clinical trials related to MSCs have been conducted in various diseases including bone defect, myocardial infarction, spinal injury, critical limb ischemia, diabetes and multiple sclerosis based on registered data at http://clinicaltrials.gov. The most of these clinical trials are being under investigation and are in phase I and II. There are several diseases of the liver that cause liver dysfunction due to hepatocytes injury and loss including viral hepatitis, fatty liver disease, drug or toxin induced liver injury, hepatocellular carcinoma, autoimmuneassociated hepatic disorders and cirrhosis. In this review, we focus on mesenchymal stem cell (MSCs) toward liver disease treatment by MSCs therapy. In this regard, we summarized characteristics of mesenchymal stem cell (MSCs) including sources, general characteristics, differentiation potential, niches, and biological effects of the cells. We also emphasized on current methods for hepatocyte differentiation and current board clinical applications. The current studies of MSCs both in preclinical and clinical trials related to liver diseases are also discussed in context of possible application in therapeutic purposes in this review. Understanding the basic knowledge of MSCs and their therapeutic capability in preclinical and clinical studies will accelerate therapeutic value of MSCs transplantations for disease treatments, drug screening, stem cell banking and regenerative medicine.

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“…Total RNA was purified from cells using NucleoSpin Tissue (Takara, Shiga, Japan), and 500 ng of total RNA was used for the reverse-transcription reaction with ReverTra Ace qPCR RT Master Mix and gDNA Remover (TOYOBO, Osaka, Japan), according to the differentiation capability, and higher expression of many pluripotent markers such as OCT3/4, SOX2, NANOG, and REX1 than dental pulp stem cells in permanent teeth (9,12). In addition, we have previously detected the expression of CD271 as an MSC marker in the deciduous teeth stem cells, but not in the stem cells from permanent teeth or MSCs from periodontal ligament and gingival connective tissues (11,21).…”

Section: Rna Purification and Analysis By Reverse Transcription Polymmentioning

confidence: 99%

<b>Characterization of mesenchymal progenitor cells in the crown and root pulp of primary </b><b>teeth </b>

et al. 2015

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The existence of progenitor/mesenchymal stem cells (MSCs) was demonstrated previously in human primary/deciduous teeth. In this study, we examined dental pulp cells from root portion (root cells) of primary teeth without discernible root resorption and compared them with pulp cells from the crown portion (crown cells). Root cells and crown cells were characterized and compared to each other based on progenitor/MSC characteristics and on their generation efficiency of induced pluripotent stem (iPS) cells. Root cells and crown cells included cells manifesting typical progenitor/MSC properties such as osteogenic and adipogenic differentiation potential and clonogenicity. Interestingly, root cells showed a higher expression level of embryonic stem cell marker, KLF4, than crown cells. Moreover, the number of colony-forming unit-fibroblast and cell proliferation rate were higher for root cells than crown cells, and the efficiency of generating iPS cells from root cells was approximately four times higher than that from crown cells. Taken together, these results suggest that root cells from primary teeth show the MSC-like properties and thus could be a potent alternative source for iPS cell generation and the subsequent transplantation therapy.

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Deciduous and Permanent Dental Pulp Mesenchymal Cells Acquire Hepatic Morphologic and Functional Features In Vitro

Cited by 116 publications

References 22 publications

Culture of human dental pulp cells at variable times post-tooth extraction

Culture of human dental pulp cells at variable times post-tooth extraction

Mesenchymal Stem Cells: Current Clinical Applications and Therapeutic Potential in Liver Diseases

<b>Characterization of mesenchymal progenitor cells in the crown and root pulp of primary </b><b>teeth </b>

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