Dechorionation as a tool to improve the fish embryo toxicity test (FET) with the zebrafish (Danio rerio)

Henn, Kirsten; Braunbeck, Thomas

doi:10.1016/j.cbpc.2010.09.003

Cited by 147 publications

(110 citation statements)

References 19 publications

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“…5) by piercing the chorion gently and-after internal pressure adjustmentenlarging the cleft carefully by means of the forceps. Lifting the chorion and turning it upside down allows the embryo to drop out of its chorions, as previously reported (21).…”

Section: Embryo Dechorionation and Maintenancementioning

confidence: 57%

TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity

Galindo-Villegas

Montalban-Arques

Liarte

et al. 2016

The Journal of Immunology

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As an organism is exposed to pathogens during very early development, specific defense mechanisms must take effect. In this study, we used a germ-free zebrafish embryo model to show that osmotic stress regulates the activation of immunity and host protection in newly hatched embryos. Mechanistically, skin keratinocytes were responsible for both sensing the hyposmolarity of the aquatic environment and mediating immune effector mechanisms. This occurred through a transient potential receptor vanilloid 4/Ca2+/TGF-β–activated kinase 1/NF-κB signaling pathway. Surprisingly, the genes encoding antimicrobial effectors, which do not have the potential to cause tissue damage, are constitutively expressed during development, independently of both commensal microbes and osmotic stress. Our results reveal that osmotic stress is associated with the induction of developmental immunity in the absence of tissue damage and point out to the embryo skin as the first organ with full capacities to mount an innate immune response.

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Section: Embryo Dechorionation and Maintenancementioning

confidence: 57%

TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity

Galindo-Villegas

Montalban-Arques

Liarte

et al. 2016

The Journal of Immunology

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“…Further, the chorion membrane complex consists of three layers, with pore canals permeating the middle and inner layers. These channels are suggested to be the route of solute transfer and permeability, which are further influenced by lipophilicity (Wiegand et al 2000, Lammer et al 2009), molecular size (Henn and Braunbeck 2011), ionic interaction on the chorion surface (Pullela et al 2006) and the xenobiotic efflux transporter (Fischer et al 2013), besides being dependent too on environmental factors. Although all mechanisms of chorion permeability are not fully understood, the most compelling approach is based on the ionic composition of the chorion surface; acting as ion exchanger or barrier depending on the binding molecule.…”

Section: Choice Of a Zebrafish Life-stage For Estrogenic Evaluationmentioning

confidence: 99%

“…For direct exposure to ambient factors by an embryo, a practice of dechorionation by physical means (Westerfield 2007, Henn andBraunbeck 2011) as well as by automated techniques (Mandrell et al 2012) have been introduced. Technically, dechorionated embryos (24 hpf) and eleutheroembryos showed similar toxicity response to exposure by Luviquat, a catioinic polymer (Henn and Braunbeck 2011), though being labour-intensive may not be practical for routine toxicity testing.…”

Section: Choice Of a Zebrafish Life-stage For Estrogenic Evaluationmentioning

confidence: 99%

“…Technically, dechorionated embryos (24 hpf) and eleutheroembryos showed similar toxicity response to exposure by Luviquat, a catioinic polymer (Henn and Braunbeck 2011), though being labour-intensive may not be practical for routine toxicity testing. Further, no dechorionation takes place in nature, which implies that this is an atypical route to elicit toxic response in the embryo, even if it may hold relevance in basic developmental biology.…”

Section: Choice Of a Zebrafish Life-stage For Estrogenic Evaluationmentioning

confidence: 99%

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Probing the xeno-oestrogenic impact of sediment cores contaminated by the pulp and paper industry: induction of aromatase cyp19a1b in late larval zebrafish

Sahoo

Oikari

2014

J Soils Sediments

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To assess xenoestrogenicity, the 20 days-post-fertilization zebrafish (20dpfZF) life-stage was experimentally exposed to known model chemicals as well as to environmentally contaminated samples. From a selection of key responsive genes, molecular responses associated with the steroidogenic impact (brain aromatase, cyp19a1b and vitellogenin isoform 1, vtg1) were used to assess the endocrinic modulation by xenoestrogenic samples. This work showed diverse in vivo responses by the 20dpfZF, from pure chemicals (bisphenol A, BPA; 17 -ethinylestradiol, EE2; nonylphenol, NP; octylphenol, OP), and their mixture combinations, and to industrially contaminated sediment samples. Gene expression analysis of cyp19a1b and vtg1 in exposed 20dpfZF showed the estrogenic potencies of chemicals in the order: EE2 > OP > BPA > NP. Transcriptional up-regulation of vtg1 in early juvenile fish exposed to mixtures (BPA + NP + OP), relative to individual concentrations, suggest in minimum the additive in vivo effect of a mixture of very low sublethal doses of single xenoestrogens. Complementary expression of cyp19a1b and vtg1 in 20dpfZF, exposed to artificially-spiked sediment with EE2 established the estrogenresponsiveness of the model. In contrast, the lack of simple estrogenic or antiestrogenic trait of correspondence in biomarker transcription was evident from exposure to two types of industrially contaminated sediments, suggesting common non-linearity of in vivo response by the model. The results also highlight the applicability of 20dpfZF in the assessment of remediation and monitoring of historically polluted sites. This study showed that the 20dpfZF addressed the regulatory requirement of a multi-purpose and cost-effective ecotoxicological model capable of in vivo genomic assessment of endocrineactive substances in aquatic samples.

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“…34 Given the expansive time period since the evolutionary divergence, it is not surprising that there are significant differences between zebrafish and mammals including the following: dependency on external heat (ectothermic); lack of cardiac septa, synovial joints, cancellous (spongy or trabecular) bone, arms and legs, and lungs; [35][36][37] and possession of a chorionic membrane that might provide a diffusion barrier to particular chemicals. [38][39][40][41] Despite these differences, a small study on 18 toxic compounds reported that toxicity in zebrafish correlated positively with toxicity in rodents. 42 A number of other studies have examined concordance between zebrafish and mammalian embryo toxicity, showing the concordance percentages of 87% (31 compounds), 43 64% (14 compounds), 44 89% (85 compounds), 45 100% (6 compounds), 46 and 55% (271 compounds).…”

mentioning

confidence: 99%

QSAR molecular parameters calculated for US EPA ToxCast Phase 1 and 2 chemical compounds tested against embryonic zebrafish

Garg

Smith

2017

Toxicology Research and Application

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Freshwater and marine environments are exposed to small concentrations of many different chemicals produced by industrial, agricultural, pharmaceutical, cosmetic, food, and household applications. Due to concerns regarding potential adverse events from these exposures, regulatory agencies around the world have established aquatic toxicology testing protocols that measure untoward responses in a wide variety of freshwater and marine organisms. Following a literature review of databases on the toxicity of chemicals to fish, the embryonic zebrafish (Danio rerio) database compiled by the Tanguay Laboratory at Oregon State University was determined to be well suited for quantitative structure-activity relationship (QSAR) analysis. This database possesses a number of favorable characteristics including large size (1060 unique US Environmental Protection Agency ToxCast phase 1 and 2 chemical compounds), relatively recent data collected using state-of-the-art methods, 18 simultaneously measured toxicological end points, transparent embryos that develop externally thereby facilitating toxicological evaluation, and the vast majority of the genetic code is expressed and active during early life stages. The molecular parameters calculated for each of the chemicals in the database include the logarithm of the octanol-water partition coefficient, molar volume, and molar refractivity. For each chemical, the availability of these molecular parameter values can facilitate future QSAR studies using any of the 18 different toxicological end points measured as the biological activity of interest.

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Dechorionation as a tool to improve the fish embryo toxicity test (FET) with the zebrafish (Danio rerio)

Cited by 147 publications

References 19 publications

TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity

TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity

Probing the xeno-oestrogenic impact of sediment cores contaminated by the pulp and paper industry: induction of aromatase cyp19a1b in late larval zebrafish

QSAR molecular parameters calculated for US EPA ToxCast Phase 1 and 2 chemical compounds tested against embryonic zebrafish

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