Deceptively simple but simply deceptive – <i>Caenorhabditis elegans</i> lifespan studies: Considerations for aging and antioxidant effects

Gruber, Jan; Ng, Li Fang; Poovathingal, Suresh; Halliwell, Barry

doi:10.1016/j.febslet.2009.09.051

Cited by 105 publications

(94 citation statements)

References 137 publications

(238 reference statements)

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“…Increased antioxidant levels and decreased ROS generation can play important roles towards better healthspan (Gruber et al 2008(Gruber et al , 2009). Our observation of increased healthspan in Drosophila after their prolonged exposure to DCA as evidenced by their improved locomotor performance with an advancement of age supports the above discussion.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, life expectancy may be improved by the amelioration of age-related behavioral declines (Jones and Grotewiel 2011). Strategies for promoting heathspan via reduced ROS generation and increased antioxidants level have gained much interest (Gruber et al 2008(Gruber et al , 2009. Among the antioxidants, glutathione (GSH) is the most abundant nonprotein thiol that protects the organism against oxidative stress and participates in the detoxification of xenobiotics and/or their metabolites, antioxidant defense, maintenance of redox potential, regulation of cell cycle progression, and apoptosis (Lu 2013).…”

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confidence: 99%

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Long-term dietary exposure to low concentration of dichloroacetic acid promoted longevity and attenuated cellular and functional declines in aged Drosophila melanogaster

Pandey

Vimal

Chandra

et al. 2014

AGE

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Dichloroacetic acid (DCA), a water disinfection by-product, has attained emphasis due to its prospect for clinical use against different diseases including cancer along with negative impact on organisms. However, these reports are based on the toxicological as well clinical data using comparatively higher concentrations of DCA without much of environmental relevance. Here, we evaluate cellular as well as organismal effects of DCA at environmentally and mild clinically relevant concentrations (0.02-20.0 μg/ml) using an established model organism, Drosophila melanogaster. Flies were fed on food mixed with test concentrations of DCA for 12-48 h to examine the induction of reactive oxygen species (ROS) generation, oxidative stress (OS), heat shock genes (hsps) and cell death along with organismal responses. We also examined locomotor performance, ROS generation, glutathione (GSH) depletion, expression of GSH-synthesizing genes (gclc and gclm), and hsps at different days (0, 10, 20, 30, 40, 50) of the age in flies after prolonged DCA exposure. We observed mild OS and induction of antioxidant defense system in 20.0 μg/ml DCA-exposed organism after 24 h. After prolonged exposure to DCA, exposed organism exhibited improved survival, elevated expression of hsp27, gclc, and gclm concomitant with lower ROS generation and GSH depletion and improved locomotor performance. Conversely, hsp27 knockdown flies exhibited reversal of the above end points. The study provides evidence for the attenuation of cellular and functional decline in aged Drosophila after prolonged DCA exposure and the effect of hsp27 modulation which further incites studies towards the therapeutic application of DCA.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Long-term dietary exposure to low concentration of dichloroacetic acid promoted longevity and attenuated cellular and functional declines in aged Drosophila melanogaster

Pandey

Vimal

Chandra

et al. 2014

AGE

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“…The Mechanisms underlying the probiotic effects of Lactic acid bacteria are also unclear. In this respect, studies on C. elegans have delivered valuable perceptions into the functional aspects of anti-aging and defense against specific pathogen ( P. aeruginosa ) and have led to the exploration of the multiple genetic pathways that determine longevity [11,12]. In addition, lactic acid bacteria are widely used and produced commercially, and they are mainly consumed in the form of fermented foods or dairy products [13].…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo defensive effect of probiotic LAB against Pseudomonas aeruginosa using Caenorhabditis elegans model

et al. 2018

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This study aimed to investigate in vitro and in vivo the probiotic characteristics of lactic acid bacteria (LAB) isolated from Korean traditional fermented foods. Caenorhabditis elegans (C. elegans) was used for analytical assays of fertility, chemotaxis, life-span, worm-killing and bacterial colonization in the intestinal lumen of the worm. All 35 strains of LAB reduced fertility and slowed development in the worms. The worm-killing assay showed that LAB significantly increased the lifespan (P < 0.05) and reduced the susceptibility to virulent PA14; however, the heat-killed LAB did not. The bacterial colonization assay revealed that LAB proliferated and protected the gut of the worm against infection by Pseudomonas aeruginosa PA14. In addition, specific LAB Pediococcus acidilactici(P. acidilactici DM-9), Pediococcus brevis (L. brevis SDL1411), and Pediococcus pentosaceus (P. pentosaceus SDL1409) strains showed acid resistance (66–91%), resistance to pepsin (64–67%) and viability in simulated intestinal fluid (67–73%) based on in vitro probiotic analyses. Taken together, these results suggest that C. elegans may be a tractable model for screening efficient probiotics.

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“…The mev-1 mutants have increased production of reactive oxygen species and have shortened life spans [1] while daf-2 mutants that have increased antioxidant genes, including a gene for Superoxide Dismutase (SOD) and mutations in the Insulin/Insulin-like Growth Factor (IGF) receptor have longer life spans compared to wild type strains [1][2][3]. One study has found that when mbk-1 is lost, the lifespan of daf-2 is shortened.…”

mentioning

confidence: 99%

“…The mechanisms involved in aging in C. elegans have also been studied in other organisms, including mammals. Among these mechanisms are oxidative damage, dietary restriction, reduction in the Insulin/Insulin-like Growth Factor (IGF) signaling pathway and decreased TOR (Target of Rapamycin) signaling [1][2][3][4]. …”

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confidence: 99%

The Study of Aging in Caenorhabditis elegans

Norflus¹

2017

AND

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Because humans have a long lifespan relative to other organisms, aging studies have been performed in a variety of systems. Caenorhabditis elegans (C. elegans) is one model organism that has been used to study aging due to its short life span (about 3 weeks), low cost for maintenance, its ease of cultivation and the fact that it produces large number of offspring [1-2]. The mechanisms involved in aging in C. elegans have also been studied in other organisms, including mammals. Among these mechanisms are oxidative damage, dietary restriction, reduction in the Insulin/Insulin-like Growth Factor (IGF) signaling pathway and decreased TOR (Target of Rapamycin) signaling [1][2][3][4].

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Deceptively simple but simply deceptive – Caenorhabditis elegans lifespan studies: Considerations for aging and antioxidant effects

Cited by 105 publications

References 137 publications

Long-term dietary exposure to low concentration of dichloroacetic acid promoted longevity and attenuated cellular and functional declines in aged Drosophila melanogaster

Long-term dietary exposure to low concentration of dichloroacetic acid promoted longevity and attenuated cellular and functional declines in aged Drosophila melanogaster

In vitro and in vivo defensive effect of probiotic LAB against Pseudomonas aeruginosa using Caenorhabditis elegans model

The Study of Aging in Caenorhabditis elegans

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