Decellularized Wharton’s jelly scaffold enhances differentiation of mesenchymal stem cells to insulin-secreting cells

Azarbarz, Nastaran; Khorsandi, Layasadat; Nejaddehbashi, Fereshteh; Neisi, Niloofar; Nejad, Darioush Bijan

doi:10.1016/j.tice.2022.101938

Cited by 8 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another study, some decellularized matrices were used as a scaffold to support the differentiation of WJ-MSCs into IPCs. A decellularized umbilical cord scaffold enhanced the expression of GLUT-2 and PDX-1 in the IPCs, while insulin gene expression and protein secretion were enhanced in comparison with cells non-cultured on decellularized matrix [ 108 ].…”

Section: Perinatal Cellsmentioning

confidence: 99%

Perinatal Stem Cell Therapy to Treat Type 1 Diabetes Mellitus: A Never-Say-Die Story of Differentiation and Immunomodulation

Paris

Pizzuti

Marrazzo

et al. 2022

IJMS

View full text Add to dashboard Cite

Human term placenta and other postpartum-derived biological tissues are promising sources of perinatal cells with unique stem cell properties. Among the massive current research on stem cells, one medical focus on easily available stem cells is to exploit them in the design of immunotherapy protocols, in particular for the treatment of chronic non-curable human diseases. Type 1 diabetes is characterized by autoimmune destruction of pancreatic beta cells and perinatal cells can be harnessed both to generate insulin-producing cells for beta cell replenishment and to regulate autoimmune mechanisms via immunomodulation capacity. In this study, the strong points of cells derived from amniotic epithelial cells and from umbilical cord matrix are outlined and their potential for supporting cell therapy development. From a basic research and expert stem cell point of view, the aim of this review is to summarize information regarding the regenerative medicine field, as well as describe the state of the art on possible cell therapy approaches for diabetes.

show abstract

Section: Perinatal Cellsmentioning

confidence: 99%

Perinatal Stem Cell Therapy to Treat Type 1 Diabetes Mellitus: A Never-Say-Die Story of Differentiation and Immunomodulation

Paris

Pizzuti

Marrazzo

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…9,10 In the current framework, the idea, at the base of the current article, is to make an attempt to ameliorate the functionality of the IVD cells embedded in a degenerated disc environment, by exploiting the properties of a specific ECM with high potential in regenerative medicine, namely the decellularized matrix obtained from the umbilical cord Wharton's jelly (DWJ). 11,12 It is indeed well known that DWJ possesses several properties well suitable for tissue repair/ engineering approaches, including biomechanical, immunomodulatory, antibacterial, and biocompatibility features. 13 The human Wharton's jelly, in the last few years, has indeed received an increasing attention due to its potential for regenerative medicine applications as "natural constituent" to produce biomimetic scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Wharton's jelly‐derived multifunctional hydrogels: New tools to promote intervertebral disc regeneration in vitro and ex vivo

Penolazzi,

Chierici,

Notarangelo

et al. 2024

J Biomedical Materials Res

View full text Add to dashboard Cite

The degeneration of intervertebral disc (IVD) is a disease of the entire joint between two vertebrae in the spine caused by loss of extracellular matrix (ECM) integrity, to date with no cure. The various regenerative approaches proposed so far have led to very limited successes. An emerging opportunity arises from the use of decellularized ECM as a scaffolding material that, directly or in combination with other materials, has greatly facilitated the advancement of tissue engineering. Here we focused on the decellularized matrix obtained from human umbilical cord Wharton's jelly (DWJ) which retains several structural and bioactive molecules very similar to those of the IVD ECM. However, being a viscous gel, DWJ has limited ability to retain ordered structural features when considered as architecture scaffold. To overcome this limitation, we produced DWJ‐based multifunctional hydrogels, in the form of 3D millicylinders containing different percentages of alginate, a seaweed‐derived polysaccharide, and gelatin, denatured collagen, which may impart mechanical integrity to the biologically active DWJ. The developed protocol, based on a freezing step, leads to the consolidation of the entire polymeric dispersion mixture, followed by an ionic gelation step and a freeze‐drying process. Finally, a porous, stable, easily storable, and suitable matrix for ex vivo experiments was obtained. The properties of the millicylinders (Wharton's jelly millicylinders [WJMs]) were then tested in culture of degenerated IVD cells isolated from disc tissues of patients undergoing surgical discectomy. We found that WJMs with the highest percentage of DWJ were effective in supporting cell migration, restoration of the IVD phenotype (increased expression of Collagen type 2, aggrecan, Sox9 and FOXO3a), anti‐inflammatory action, and stem cell activity of resident progenitor/notochordal cells (increased number of CD24 positive cells). We are confident that the DWJ‐based formulations proposed here can provide adequate stimuli to the cells present in the degenerated IVD to restart the anabolic machinery.

show abstract

“…При создании КИК/ТИК in vitro важным условием для достижения высокой степени их сходства с естественной тканью является воспроизведение не только биохимических, но и биомеханических стимулов, обеспечивающих жизнедеятельность клеток в организме [ [18,19,24,25,[31][32][33]37], в 4 -только исследование in vivo (на животных) [34,41,43,44], и в 10 исследованиях были проведены оба типа доклинических испытаний [11,16,17,20,21,26,35,36,38,42]. Клинических исследований, отвечающих критериям включения, найдено не было.…”

unclassified

Decellularized umbilical cord stroma in tissue engineering and regenerative medicine: a systematic review

Basok

Kondratenko

Kalyuzhnaya

et al. 2023

RJTAO

View full text Add to dashboard Cite

Despite great progress in the field of biomaterials for tissue engineering and regenerative medicine, the high requirements placed on artificial matrices (matrices, carriers, scaffolds) are the reason for the ongoing search for natural or synthetic extracellular matrix mimetics. Among such materials, decellularized umbilical cord (UC) stroma appears to be very attractive – it has a high content of hyaluronic acid, cytokines, and growth factors, and there are no ethical restrictions for its production. Decellularized UC stroma has been found to promote cartilage, liver tissue and nerve tissue repair, as well as wound healing. The review critically analyzes and summarizes published data on the ability of decellularized UC stroma to maintain the necessary conditions for adhesion, migration, differentiation and functional activity of adherent cells, thus stimulating the internal (physiological) regenerative potential of tissues. Literature was searched for in the following electronic databases: Medline/PubMed (www/ncbi. nlm.nih.gov/pubmed), Cochrane library (https://www.cochrane.org), and eLIBRARY/Russian Science Citation Index (https://www.elibrary.ru). Inclusion criteria were the presence of biomaterials obtained from decellularized human UC stroma. Exclusion criteria for papers included research objects as decellularized umbilical cord vessels (veins and arteries) and umbilical cord cell cultures. Twenty-five original articles in English and Russian were selected for analysis of the products obtained, their applications, decellularization methods and research results. The review also discusses the prospects for decellularized umbilical cord in medicine.

show abstract

Decellularized Wharton’s jelly scaffold enhances differentiation of mesenchymal stem cells to insulin-secreting cells

Cited by 8 publications

References 33 publications

Perinatal Stem Cell Therapy to Treat Type 1 Diabetes Mellitus: A Never-Say-Die Story of Differentiation and Immunomodulation

Perinatal Stem Cell Therapy to Treat Type 1 Diabetes Mellitus: A Never-Say-Die Story of Differentiation and Immunomodulation

Wharton's jelly‐derived multifunctional hydrogels: New tools to promote intervertebral disc regeneration in vitro and ex vivo

Decellularized umbilical cord stroma in tissue engineering and regenerative medicine: a systematic review

Contact Info

Product

Resources

About