Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation

Abstract

Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues. In this study, decellularized bovine spinal cord meninges (dSCM)derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges. The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL. Frequency and temperature sweep rheometry show that dSCM forms self-supporting hydrogel at physiological temperature. The MeninGEL possesses excellent cytocompatibility. Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neurallineage cells. Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching. With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.

Tuning the Cell-Adhesive Properties of Two-Component Hybrid Hydrogels to Modulate Cancer Cell Behavior, Metastasis, and Death Pathways

“…The porosity (%) of hybrid hydrogels prepared in volumetric ratios of 2:1, 1:1, and 1:2 (v/v) was determined by the liquid displacement method. 27 The detailed experimental procedure is presented in the SI .…”

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

“…Stem cells have the unique ability to differentiate into different tissue types, but they generally require specialized treatments to induce this differentiation into a particular cell type. The specific make-up of the ECM is unique to each tissue type, and the ECM has been shown to have the potential to induce and enhance the differentiation of stem cells towards the phenotype associated with the tissue source of the ECM [ 14 , 56 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 ].…”

Omics technologies for high-throughput-screening of cell–biomaterial interactions

“…Decellularized biomaterials, prepared using detergents, nucleases and other reagents by the removal of intact cells and nuclear materials from tissues or organs, are such biomaterials that are used to recapitulate damaged tissues or organs after recellularization with a patient's own cells. 133 Although the application of these scaffolds is rapidly becoming widespread in tissue engineering and regenerative medicine applications, 134 the information about the components of these matrices is still not complete.…”