Decellularized scaffolds in regenerative medicine

Yu, Yaling; Alkhawaji, Ali; Ding, Yu; Mei, Jin

doi:10.18632/oncotarget.10945

Cited by 89 publications

(54 citation statements)

References 108 publications

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“…Consequently, this was selected as the optimized concentration of GO in the scaffold for the NIH 3T3 cell culture to avoid the potential for cytotoxicity. These results are in agreement with previous studies that have demonstrated that both DT constructs and GO are biocompatible and biodegradable …”

Section: Resultssupporting

confidence: 94%

“…These natural scaffolds can support angiogenesis due to their notable cell affinity and their ability to provide a microenvironment similar to the extracellular matrix . Decellularized constructs have been used for skin transplantations, cosmetic surgery, and for skin defects, and the results have confirmed that they can support skin regeneration after implantation . Decellularized scaffolds can stimulate important biological processes such as re‐epithelialization, granular tissue formation, and neovascularization at the initial stages of implantation.…”

Section: Introductionmentioning

confidence: 99%

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Graphene functionalized decellularized scaffold promotes skin cell proliferation

et al. 2019

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An increasing number of new strategies for skin tissue engineering have been developed with the potential to mimic the biological properties of native tissue with a high degree of complexity, flexibility, and reproducibility. In this study, decellularized tissue (DT) was prepared from the bovine heart by using chemical treatments. However, the mechanical properties of the DT constructs were poorer than the extra cellular matrix of the skin tissue. To overcome this challenge, hybrid scaffolds of DT and graphene oxide (GO) were developed and the effects of the GO concentration on the morphology, pore size, porosity, mechanical strength, and water uptake capacity of the samples were evaluated. Moreover, the biocompatibility of hybrid scaffolds was studied by Live/Dead staining. The results show that a hybrid scaffold incorporating 3 % graphene oxide improved the mechanical strength and cell viability by ~25 % in comparison to the DT scaffolds. Cell viability results confirmed that the porous scaffolds could support cell adhesion, proliferation, and cell activity for 7 days. This study provides new insight into and opportunities for using graphene‐based materials to develop biomimetic constructs for clinical applications.

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Section: Resultssupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Graphene functionalized decellularized scaffold promotes skin cell proliferation

et al. 2019

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“…Cardiac tissue engineering (CTE) aiming at developing three-dimensional myocardium-like scaffolds for therapeutic use by combining cells, synthetic or natural biomaterials, and biomimetic signals is rapidly emerging as the most promising alternative to heart transplant (Vunjak-Novakovic et al, 2010;Massai et al, 2013;Feric and Radisic, 2016;Stoppel et al, 2016;Fujita and Zimmermann, 2017;Weinberger et al, 2017;Madonna et al, 2019). Among biomaterials, decellularized tissues are emerging as the most promising scaffolds for regenerative medicine, due to their potential to provide natural biological cues (Yaling et al, 2016). However, the regenerative capability of the substrate depends not only on its chemical properties, but also on its ability to recapitulate the mechanical behavior of the native tissue, providing physical signals essential for mechanotransduction pathways (Huang et al, 2004;O'Brien, 2011).…”

Section: Introductionmentioning

confidence: 99%

Decellularized Human Dermal Matrix as a Biological Scaffold for Cardiac Repair and Regeneration

Belviso

Romano

Sacco

et al. 2020

Front. Bioeng. Biotechnol.

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The complex and highly organized environment in which cells reside consists primarily of the extracellular matrix (ECM) that delivers biological signals and physical stimuli to resident cells. In the native myocardium, the ECM contributes to both heart compliance and cardiomyocyte maturation and function. Thus, myocardium regeneration cannot be accomplished if cardiac ECM is not restored. We hypothesize that decellularized human skin might make an easily accessible and viable alternate biological scaffold for cardiac tissue engineering (CTE). To test our hypothesis, we decellularized specimens of both human skin and human myocardium and analyzed and compared their composition by histological methods and quantitative assays. Decellularized dermal matrix was then cut into 600-µm-thick sections and either tested by uniaxial tensile stretching to characterize its mechanical behavior or used as three-dimensional scaffold to assess its capability to support regeneration by resident cardiac progenitor cells (hCPCs) in vitro. Histological and quantitative analyses of the dermal matrix provided evidence of both effective decellularization with preserved tissue architecture and retention of ECM proteins and growth factors typical of cardiac matrix. Further, the elastic modulus of the dermal matrix resulted comparable with that reported in literature for the human myocardium and, when tested in vitro, dermal matrix resulted a comfortable and protective substrate promoting and supporting hCPC engraftment, survival and cardiomyogenic potential. Our study provides compelling evidence that dermal matrix holds promise as a fully autologous and cost-effective biological scaffold for CTE.

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“…This new application of these materials contrasts with their historical use as matrices for reconstruction, for delivering cells and biological factors, or for controlled release. Although using biologically sourced scaffolds to deliver cells and factors remains an active area of research [105], as does the use of decellularized whole organs in regenerative medicine applications [106], such usage generally lies outside the focus of this review and will be deemphasized here. Rather, in this section we will emphasize the alternative strategy of using the scaffold to stimulate productive immune and inflammatory responses.…”

Section: High Complexity: Ecm-derived Scaffolds Inducing Therapeutmentioning

confidence: 99%

Biomaterial strategies for generating therapeutic immune responses

Kelly

Shores

Votaw

et al. 2017

Advanced Drug Delivery Reviews

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Biomaterials employed to raise therapeutic immune responses have become a complex and active field. Historically, vaccines have been developed primarily to fight infectious diseases, but recent years have seen the development of immunologically active biomaterials towards an expanding list of non-infectious diseases and conditions including inflammation, autoimmunity, wounds, cancer, and others. This review structures its discussion of these approaches around a progression from single-target strategies to those that engage increasingly complex and multifactorial immune responses. First the targeting of specific individual cytokines is discussed, both in terms of delivering the cytokines or blocking agents, and in terms of active immunotherapies that raise neutralizing immune responses against such single cytokine targets. Next, non-biological complex drugs such as randomized polyamino acid copolymers are discussed in terms of their ability to raise multiple different therapeutic immune responses, particularly in the context of autoimmunity. Last, biologically derived matrices and materials are discussed in terms of their ability to raise complex immune responses in the context of tissue repair. Collectively, these examples reflect the tremendous diversity of existing approaches and the breadth of opportunities that remain for generating therapeutic immune responses using biomaterials.

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Decellularized scaffolds in regenerative medicine

Cited by 89 publications

References 108 publications

Graphene functionalized decellularized scaffold promotes skin cell proliferation

Graphene functionalized decellularized scaffold promotes skin cell proliferation

Decellularized Human Dermal Matrix as a Biological Scaffold for Cardiac Repair and Regeneration

Biomaterial strategies for generating therapeutic immune responses

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