Decellularized porcine conjunctiva as an alternative substrate for tissue-engineered epithelialized conjunctiva

Witt, Joana; Dietrich, Jana; Mertsch, Sonja; Schrader, Stefan; Spaniol, Kristina; Geerling, Gerd

doi:10.1016/j.jtos.2020.08.009

Cited by 20 publications

(33 citation statements)

References 52 publications

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“…To study conjunctival cells, various species are used as donors including rabbits, bovids, and humans [ 89 , 90 , 91 , 92 ]. In addition, described cell culture conditions vary greatly with regard to isolation, medium, carrier matrices, and differentiation protocols.…”

Section: Development Of 3d Cell Culture Modelsmentioning

confidence: 99%

“…In addition, described cell culture conditions vary greatly with regard to isolation, medium, carrier matrices, and differentiation protocols. In most cases, isolation was carried out by enzymatic homogenization of the conjunctiva with subsequent seeding [ 90 , 91 , 92 , 93 ], but also attempts were made to use whole conjunctival pieces and isolate the cells by simply letting the explant culture grow out on a suitable matrix [ 89 , 94 ]. The majority of studies use medium based on Dulbecco’s Modified Eagle Medium (DMEM)/F12 nutrient mix with fetal bovine serum, insulin, and epidermal growth factor (EGF) as additives among others for the expansion of conjunctival cells (see Table A1 , Appendix A ).…”

Section: Development Of 3d Cell Culture Modelsmentioning

confidence: 99%

“…( B ) 3D cultured human conjunctival epithelial cells growing on a transwell insert. Isolation as explant culture on transwell with cultivation (21 days) and differentiation performed according to reference [ 89 ]. E = epithelium, Su = superficial epithelial cells, I = intermediate cells, B = basal cells, S = Stroma, T = transwell; Magnification is 200× ( A ) and 400× ( B ) with 50 µm scale bar.…”

Section: Figurementioning

confidence: 99%

“… Medium Composition Ref. 1 CjSC Medium DMEM/F-12 (3:1) 10% fetal bovine serum 1% penicillin-streptomycin 1× insulin-transferrin-selenium 10 ng/mL epidermal growth factor 400 ng/mL hydrocortisone 0.1 nM cholera toxin 2 nM 3,3´,5-triiodo-L-thyronine 10 µM Y27632 1 µM A83-01 1 µM DMH1 [ 90 ] Goblet cell differentiation medium Keratinocyte SFM 1% penicillin-streptomycin 50 µg/mL bovine pituitary extract 5 ng/mL epidermal growth factor 100 ng/mL bone morphogenetic protein 4 10 ng/mL fibroblast growth factor 10 100 ng/mL interleukin 13 1 µM A83-01 2 CEC Medium DMEM/F-12 (1:1) 10% fetal bovine serum 1% penicillin-streptomycin 5 µg/mL transferrin 10 ng/mL epidermal growth factor 400 ng/mL hydrocortisone 0.1 nM cholera toxin 0.075% sodium bicarbonate 0.18 mM adenin 5 µg/mL insulin 2 nM 3,3´,5-triiodo-L-thyronine [ 89 ] Differentiation medium DMEM/F-12 (1:1) 10% fetal bovine serum 1% penicillin-streptomycin 5 µg/mL transferrin 0.5 ng/mL epidermal growth factor 400 ng/mL hydrocortisone 0.1 nM cholera toxin 0.075% sodium bicarbonate 0.18 mM adenin 5 µg/mL insulin 2 nM 3,3´,5-triiodo-L-thyronine 3 Complete growth medium DMEM/F-12 (1:1) 10% fetal bovine serum ...…”

Section: Table A1mentioning

confidence: 99%

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The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

Aydin

Dietrich

Witt

et al. 2021

IJMS

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There is a lack of knowledge regarding the connection between the ocular and nasal epithelia. This narrative review focuses on conjunctival, corneal, ultrastructural corneal stroma, and nasal epithelia as well as an introduction into their interconnections. We describe in detail the morphology and physiology of the ocular surface, the nasolacrimal ducts, and the nasal cavity. This knowledge provides a basis for functional studies and the development of relevant cell culture models that can be used to investigate the pathogenesis of diseases related to these complex structures. Moreover, we also provide a state-of-the-art overview regarding the development of 3D culture models, which allow for addressing research questions in models resembling the in vivo situation. In particular, we give an overview of the current developments of corneal 3D and organoid models, as well as 3D cell culture models of epithelia with goblet cells (conjunctiva and nasal cavity). The benefits and shortcomings of these cell culture models are discussed. As examples for pathogens related to ocular and nasal epithelia, we discuss infections caused by adenovirus and measles virus. In addition to pathogens, also external triggers such as allergens can cause rhinoconjunctivitis. These diseases exemplify the interconnections between the ocular surface and nasal epithelia in a molecular and clinical context. With a final translational section on optical coherence tomography (OCT), we provide an overview about the applicability of this technique in basic research and clinical ophthalmology. The techniques presented herein will be instrumental in further elucidating the functional interrelations and crosstalk between ocular and nasal epithelia.

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Section: Development Of 3d Cell Culture Modelsmentioning

confidence: 99%

Section: Development Of 3d Cell Culture Modelsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Table A1mentioning

confidence: 99%

See 2 more Smart Citations

The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

Aydin

Dietrich

Witt

et al. 2021

IJMS

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“…Treatments that rely on the autologous conjunctiva are difficult, especially in cases with large conjunctival defects or severe inflammatory surface disorders in both eyes. Furthermore, investigators are also increasingly aware of the limitations of autologous tissue substitutes, including the risk of virus transmission and the absence of goblet cells of donors [ 2 ]. For these reasons, the development of a suitable alternative graft material quality for conjunctiva has become the focus of increasing attention.…”

Section: Introductionmentioning

confidence: 99%

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction

Chen

Deng

Luo

et al. 2022

Stem Cells International

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Seeking for suitable conjunctival reconstruction substitutes to overcome the limitations of current substitutes, such as amniotic membrane, is urgent. Decellularized tissues have become a promising strategy for tissue engineering. In this study, we prepared decellularized porcine pericardium (DPP) scaffolds by the phospholipase A2 method and crosslinked them with aspartic acid (Asp) and human endothelial growth factor (hEGF) to enhance biological performance on the DPP, obtaining DPP-Asp-hEGF scaffolds. In vitro DPP showed lower apoptosis, highly desirable, well preservation of extracellular matrix components, and favorable macro-microstructure, which was confirmed by histology, immunofluorescence, electron microscopy, collagen and DNA quantification, and cytotoxicity assay, compared to the native porcine pericardium (NPP). The crosslinked efficacy of the DPP-Asp-hEGF was furtherer verified by in vitro experiments with Fourier transform infrared (FTIR) and X-ray diffraction (XRD). Through animal models of conjunctiva defect model, the DPP-Asp-hEGF revealed a closed, multilayer epithelium with an equal amount of goblet cells and no indication for conjunctival scarring after 28 days, compared to amniotic membrane (AM) groups and sham groups. These results suggested that DPP-Asp-hEGF can offer a good conjunctival reconstructive substitute both in structure and in function.

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Biomaterials and tissue engineering strategies for posterior lamellar eyelid reconstruction: Replacement or regeneration?

Yan

et al. 2023

Bioengineering & Transla Med

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Reconstruction of posterior lamellar eyelids remains challenging due to their delicate structure, highly specialized function, and cosmetic concerns. Current clinically available techniques for posterior lamellar reconstruction mainly focus on reconstructing the contour of the eyelids. However, the posterior lamella not only provides structural support for the eyelid but also offers a smooth mucosal surface to facilitate globe movement and secrete lipids to maintain ocular surface homeostasis. Bioengineered posterior lamellar substitutes developed via acellular or cellular approaches have shown promise as alternatives to current therapies and encouraging outcomes in animal studies and clinical conditions. Here, we provide a brief reference on the current application of autografts, biomaterials, and tissue‐engineered substitutes for posterior lamellar eyelid reconstruction. We also shed light on future challenges and directions for eyelid regeneration strategies and offer perspectives on transitioning replacement strategies to regeneration strategies for eyelid reconstruction in the future.

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Decellularized porcine conjunctiva as an alternative substrate for tissue-engineered epithelialized conjunctiva

Cited by 20 publications

References 52 publications

The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction

Biomaterials and tissue engineering strategies for posterior lamellar eyelid reconstruction: Replacement or regeneration?

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