Decellularized periosteum as a potential biologic scaffold for bone tissue engineering

Chen, Kai; Lin, Xianfeng; Zhang, Qi; Ni, Juan; Li, Jianmin; Xiao, Jian; Wang, Yan; Ye, Yiheng; Chen, Li; Jin, Keke; Chen, Lei

doi:10.1016/j.actbio.2015.02.020

Cited by 69 publications

(59 citation statements)

References 42 publications

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“…Peracetic acid is frequently used to decellularize thin tissues such as small intestine submucosa (SIS). For more dense tissues such as menisci, formic acid is considered the best choice for removing both collagen and GAGs …”

Section: General Methods Of Decellularizationmentioning

confidence: 99%

Applications of decellularized extracellular matrix in bone and cartilage tissue engineering

Kim

Majid

Melchiorri

et al. 2018

Bioengineering & Transla Med

229

175

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Regenerative therapies for bone and cartilage injuries are currently unable to replicate the complex microenvironment of native tissue. There are many tissue engineering approaches attempting to address this issue through the use of synthetic materials. Although synthetic materials can be modified to simulate the mechanical and biochemical properties of the cell microenvironment, they do not mimic in full the multitude of interactions that take place within tissue. Decellularized extracellular matrix (dECM) has been established as a biomaterial that preserves a tissue's native environment, promotes cell proliferation, and provides cues for cell differentiation. The potential of dECM as a therapeutic agent is rising, but there are many limitations of dECM restricting its use. This review discusses the recent progress in the utilization of bone and cartilage dECM through applications as scaffolds, particles, and supplementary factors in bone and cartilage tissue engineering.

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Section: General Methods Of Decellularizationmentioning

confidence: 99%

Applications of decellularized extracellular matrix in bone and cartilage tissue engineering

Kim

Majid

Melchiorri

et al. 2018

Bioengineering & Transla Med

229

175

View full text Add to dashboard Cite

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“…Alcian blue (LEAGENE, Beijing, China) were stained according to the protocol. Total amounts of proteoglycan released into the culture medium and in the ECM were also measured using the 1, 9-dimethylmethylene blue (DMMB; Sigma) colorimetric assay, as described [56]. Briefly, CEP and NP cells (3 wells/treatment) were lyophilized to a constant weight and digested in papain buffer (125 mg/ml papain, 5 mM cysteine HCl, 5 mM disodium EDTA in PBS) at 65°C for 12 h. Sample absorbance at 525 nm was measured using a microplate spectrophotometer (Thermo Scientific) immediately after adding DMMB.…”

Section: Methodsmentioning

confidence: 99%

Lycorine Suppresses Endplate-Chondrocyte Degeneration and Prevents Intervertebral Disc Degeneration by Inhibiting NF-κB Signalling Pathway

Wang

Huang

Dong

et al. 2018

Cell Physiol Biochem

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Background/Aims: Cartilaginous endplate (CEP) degeneration is an important cause for intervertebral disc (IVD) degeneration that leads to low-back pain. The identification of compounds that may prevent CEP degeneration is of interest for the prevention of IVD degeneration. Methods: Catabolic protease expression in the CEP of disc degeneration patients was first assessed. The toxicity, function and underlying mechanism of lycorine (LY) on CEP-derived chondrocytes degeneration were assessed in vitro by flow cytometry analysis and western blotting. The concentration and function of LY in rat-tail disc-degeneration models were also assessed by HPLC (High Performance Liquid Chromatography) quantification and histological analysis. Results: In CEP cells, Interleukin (IL)-1β upregulated the expression of matrix metalloproteinase (MMP)-3, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4 and ADAMTS-5 that is critical for the degradation of cartilage extracellular matrix. Interestingly, LY suppressed the expression of these enzymes via the inhibition of nuclear factor-κB (NFκB) signalling and thus prevented IL-1β-induced endplate cell degeneration in vitro. More importantly, LY also reduced the expression of MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5 in CEP and exerted a protective effect on both CEP and nucleus pulposus (NP) degeneration. In addition to its inhibitory effect on matrix-degrading protease expression, LY treatment also reduced positive regulators of proinflammatory cytokines, such as MIF, which can be secreted by CEP cells and subsequently target NP cells. Conclusion: LY could serve as a potential drug for treating IVD disease.

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“…Although autologous bone grafts are a gold standard and allograft options are extensively used to treat bone defects in the clinical setting, they have their own drawbacks [1–3], including donor-side morbidity and increased operation time [4]. A synthetic bone substitute as an alternative solution holds great promise to treat large bone defects, and has been extensively studied over the past decades [5–7].…”

Section: Introductionmentioning

confidence: 99%

Engineering biomimetic periosteum with β-TCP scaffolds to promote bone formation in calvarial defects of rats

Zhang

Gao

et al. 2017

Stem Cell Res Ther

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BackgroundThere is a critical need for the management of large bone defects. The purpose of this study was to engineer a biomimetic periosteum and to combine this with a macroporous β-tricalcium phosphate (β-TCP) scaffold for bone tissue regeneration.MethodsRat bone marrow-derived mesenchymal stem cells (rBMSCs) were harvested and cultured in different culture media to form undifferentiated rBMSC sheets (undifferentiated medium (UM)) and osteogenic cell sheets (osteogenic medium (OM)). Simultaneously, rBMSCs were differentiated to induced endothelial-like cells (iECs), and the iECs were further cultured on a UM to form a vascularized cell sheet. At the same time, flow cytometry was used to detect the conversion rates of rBMSCs to iECs. The pre-vascularized cell sheet (iECs/UM) and the osteogenic cell sheet (OM) were stacked together to form a biomimetic periosteum with two distinct layers, which mimicked the fibrous layer and cambium layer of native periosteum. The biomimetic periostea were wrapped onto porous β-TCP scaffolds (BP/β-TCP) and implanted in the calvarial bone defects of rats. As controls, autologous periostea with β-TCP (AP/β-TCP) and β-TCP alone were implanted in the calvarial defects of rats, with a no implantation group as another control. At 2, 4, and 8 weeks post-surgery, implants were retrieved and X-ray, microcomputed tomography (micro-CT), histology, and immunohistochemistry staining analyses were performed.ResultsFlow cytometry results showed that rBMSCs were partially differentiated into iECs with a 35.1% conversion rate in terms of CD31. There were still 20.97% rBMSCs expressing CD90. Scanning electron microscopy (SEM) results indicated that cells from the wrapped cell sheet on the β-TCP scaffold apparently migrated into the pores of the β-TCP scaffold. The histology and immunohistochemistry staining results from in vivo implantation indicated that the BP/β-TCP and AP/β-TCP groups promoted the formation of blood vessels and new bone tissues in the bone defects more than the other two control groups. In addition, micro-CT showed that more new bone tissue formed in the BP/β-TCP and AP/β-TCP groups than the other groups.ConclusionsInducing rBMSCs to iECs could be a good strategy to obtain an endothelial cell source for prevascularization. Our findings indicate that the biomimetic periosteum with porous β-TCP scaffold has a similar ability to promote osteogenesis and angiogenesis in vivo compared to the autologous periosteum. This function could result from the double layers of biomimetic periosteum. The prevascularized cell sheet served a mimetic fibrous layer and the osteogenic cell sheet served a cambium layer of native periosteum. The biomimetic periosteum with a porous ceramic scaffold provides a new promising method for bone healing.

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Decellularized periosteum as a potential biologic scaffold for bone tissue engineering

Cited by 69 publications

References 42 publications

Applications of decellularized extracellular matrix in bone and cartilage tissue engineering

Applications of decellularized extracellular matrix in bone and cartilage tissue engineering

Lycorine Suppresses Endplate-Chondrocyte Degeneration and Prevents Intervertebral Disc Degeneration by Inhibiting NF-κB Signalling Pathway

Engineering biomimetic periosteum with β-TCP scaffolds to promote bone formation in calvarial defects of rats

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