Decellularized nerve matrix hydrogel and glial‐derived neurotrophic factor modifications assisted nerve repair with decellularized nerve matrix scaffolds

Qiu, Shuai; Rao, Zilong; He, Fu-Lin; Wang, Tao; Xu, Yin; Du, Zhaoyi; Yao, Zhaolin; Lin, Tao; Yan, Liwei; Quan, Daping; Zhu, Qian; Liu, Xiaolin

doi:10.1002/term.3050

Cited by 30 publications

(19 citation statements)

References 39 publications

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“…Irradiation can directly destroy the nucleic acid, protein and enzyme in the microorganism, due to its strong penetration capability. However, irradiation could lead to changes in physical properties, chemical properties, and biological compatibility of dECM [ 156 , 157 ]. Ethylene oxide (EO) sterilization is a relatively mature sterilization method, which can make the amino and carboxyl groups in microbial protein and nucleic acid alkylation, and inactivate the macromolecules [ 158 ].…”

Section: Classification and Fabrication Of Decm Scaffoldsmentioning

confidence: 99%

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Zhang

Chen

Hong

et al. 2022

Bioactive Materials

339

263

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The application of scaffolding materials is believed to hold enormous potential for tissue regeneration. Despite the widespread application and rapid advance of several tissue-engineered scaffolds such as natural and synthetic polymer-based scaffolds, they have limited repair capacity due to the difficulties in overcoming the immunogenicity, simulating in-vivo microenvironment, and performing mechanical or biochemical properties similar to native organs/tissues. Fortunately, the emergence of decellularized extracellular matrix (dECM) scaffolds provides an attractive way to overcome these hurdles, which mimic an optimal non-immune environment with native three-dimensional structures and various bioactive components. The consequent cell-seeded construct based on dECM scaffolds, especially stem cell-recellularized construct, is considered an ideal choice for regenerating functional organs/tissues. Herein, we review recent developments in dECM scaffolds and put forward perspectives accordingly, with particular focus on the concept and fabrication of decellularized scaffolds, as well as the application of decellularized scaffolds and their combinations with stem cells (recellularized scaffolds) in tissue engineering, including skin, bone, nerve, heart, along with lung, liver and kidney.

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Section: Classification and Fabrication Of Decm Scaffoldsmentioning

confidence: 99%

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Zhang

Chen

Hong

et al. 2022

Bioactive Materials

339

263

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“…Previous studies investigating nerve decellularization mainly used 3% Triton™ X-100 and 4% SDC for up to 72 h [ 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ]. By following our hypothesis of combining detergents, we could reduce these concentrations to 1% for Triton™ X-100 and 2% for SDC.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization

Kuna

Lundgren

Anerillas

et al. 2022

IJMS

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Injuries to large peripheral nerves are often associated with tissue defects and require reconstruction using autologous nerve grafts, which have limited availability and result in donor site morbidity. Peripheral nerve-derived hydrogels could potentially supplement or even replace these grafts. In this study, three decellularization protocols based on the ionic detergents sodium dodecyl sulfate (P1) and sodium deoxycholate (P2), or the organic solvent tri-n-butyl phosphate (P3), were used to prepare hydrogels. All protocols resulted in significantly decreased amounts of genomic DNA, but the P2 hydrogel showed the best preservation of extracellular matrix proteins, cytokines, and chemokines, and reduced levels of sulfated glycosaminoglycans. In vitro P1 and P2 hydrogels supported Schwann cell viability, secretion of VEGF, and neurite outgrowth. Surgical repair of a 10 mm-long rat sciatic nerve gap was performed by implantation of tubular polycaprolactone conduits filled with hydrogels followed by analyses using diffusion tensor imaging and immunostaining for neuronal and glial markers. The results demonstrated that the P2 hydrogel considerably increased the number of axons and the distance of regeneration into the distal nerve stump. In summary, the method used to decellularize nerve tissue affects the efficacy of the resulting hydrogels to support regeneration after nerve injury.

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“…Shuai et al have successfully developed a human decellularized nerve scaffold via combining decellularized nerve matrix hydrogel and glial-derived neurotrophic factor together and applying it to bridge a 50 mm sciatic nerve defect in a beagle model. The result showed that this nerve scaffold had excellent effects on promoting motor function recovery and nerve tissue remodeling (85). Additionally, studies have confirmed that alginate/hyaluronic acid 3d scaffold was used successfully to direct the differentiation of encapsulated gingival mesenchymal stem cells towards neurogenic tissues for nerve regeneration therapies (86).…”

Section: Application Of Ecm-p In Regenerative Medicinementioning

confidence: 94%

Extracellular matrix grafts: From preparation to application (Review)

Jiang

Han

et al. 2020

Int J Mol Med

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Recently, the increasing emergency of traffic accidents and the unsatisfactory outcome of surgical intervention are driving research to seek a novel technology to repair traumatic soft tissue injury. From this perspective, decellularized matrix grafts (ECM-G) including natural ECM materials, and their prepared hydrogels and bioscaffolds, have emerged as possible alternatives for tissue engineering and regenerative medicine. Over the past decades, several physical and chemical decellularization methods have been used extensively to deal with different tissues/organs in an attempt to carefully remove cellular antigens while maintaining the non-immunogenic ECM components. It is anticipated that when the decellularized biomaterials are seeded with cells in vitro or incorporated into irregularly shaped defects in vivo , they can provide the appropriate biomechanical and biochemical conditions for directing cell behavior and tissue remodeling. The aim of this review is to first summarize the characteristics of ECM-G and describe their major decellularization methods from different sources, followed by analysis of how the bioactive factors and undesired residual cellular compositions influence the biologic function and host tissue response following implantation. Lastly, we also provide an overview of the in vivo application of ECM-G in facilitating tissue repair and remodeling.

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Decellularized nerve matrix hydrogel and glial‐derived neurotrophic factor modifications assisted nerve repair with decellularized nerve matrix scaffolds

Cited by 30 publications

References 39 publications

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization

Extracellular matrix grafts: From preparation to application (Review)

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