Decellularized Liver Extracellular Matrix as Promising Tools for Transplantable Bioengineered Liver Promotes Hepatic Lineage Commitments of Induced Pluripotent Stem Cells

Park, Kyung-Mee; Hussein, Kamal Hany; Hong, Seok‐Ho; Ahn, Changwoo; Yang, Se‐Ran; Park, Sung Min; Kweon, Oh‐Kyeong; Kim, Byeong–Moo; Woo, Heung‐Myong

doi:10.1089/ten.tea.2015.0313

Cited by 97 publications

(111 citation statements)

References 55 publications

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“…Gel electrophoresis of UBM hydrogels shows similar bands to SIS hydrogels and both show additional bands corresponding to other ECM proteins [20]. Intact growth factors have also been confirmed in adipose [50], colon [51], liver [52], and SIS [53] ECM hydrogels, although present in reduced amounts compared to native tissue or ECM scaffolds. The impact of solubilization on cryptic peptide and matrix-bound nanovesicle content or activity has yet to be evaluated.…”

Section: Ecm Hydrogel Characterizationmentioning

confidence: 99%

Extracellular matrix hydrogels from decellularized tissues: Structure and function

et al. 2017

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Extracellular matrix (ECM) bioscaffolds prepared from decellularized tissues have been used to facilitate constructive and functional tissue remodeling in a variety of clinical applications. The discovery that these ECM materials could be solubilized and subsequently manipulated to form hydrogels expanded their potential in vitro and in vivo utility; i.e. as culture substrates comparable to collagen or Matrigel, and as injectable materials that fill irregularly-shaped defects. The mechanisms by which ECM hydrogels direct cell behavior and influence remodeling outcomes are only partially understood, but likely include structural and biological signals retained from the native source tissue. The present review describes the utility, formation, and physical and biological characterization of ECM hydrogels. Two examples of clinical application are presented to demonstrate in vivo utility of ECM hydrogels in different organ systems. Finally, new research directions and clinical translation of ECM hydrogels are discussed.

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Section: Ecm Hydrogel Characterizationmentioning

confidence: 99%

Extracellular matrix hydrogels from decellularized tissues: Structure and function

et al. 2017

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“…Due to the unlimited selfrenewing capacity and less immunogenicity of iPSCs, they became a preferred choice for hepatocyte generation. 13 In this present study, we hypothesize that the decellularized WP scaffold (DWJS) prepared in vitro may be an ideal scaffold for cultivating and differentiating iPSCs to hepatocytes. 28,29 However, there is only one report describing the use of hiPSCs-derived hepatocytes (hiPSC-Heps) to repopulate decellularized scaffolds.…”

mentioning

confidence: 90%

“…In the case of liver, primary hepatocytes are mainly used to reseed decellularized liver scaffolds; 10,13,20,21 however, utilizing them is challenging because of limited sources, slow growth, and loss of function in vitro. In the case of liver, primary hepatocytes are mainly used to reseed decellularized liver scaffolds; 10,13,20,21 however, utilizing them is challenging because of limited sources, slow growth, and loss of function in vitro.…”

mentioning

confidence: 99%

Decellularized Wharton's jelly extracellular matrix as a promising scaffold for promoting hepatic differentiation of human induced pluripotent stem cells

Kehtari

Beiki

Zeynali

et al. 2018

J of Cellular Biochemistry

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Liver tissue engineering as a therapeutic option for restoring of damaged liver function has a special focus on using native decellularized liver matrix, but there are limitations such as the shortage of liver donor. Therefore, an appropriate alternative scaffold is needed to circumvent the donor shortage. This study was designed to evaluate hepatic differentiation of human induced pluripotent stem cells (hiPSCs) in decellularized Wharton's jelly (WJ) matrix as an alternative for native liver matrix. WJ matrices were treated with a series of detergents for decellularization. Then hiPSCs were seeded into decellularized WJ scaffold (DWJS) for hepatic differentiation by a defined induction protocol. The DNA quantitative assay and histological evaluation showed that cellular and nuclear materials were efficiently removed and the composition of extracellular matrix was maintained. In DWJS, hiPSCs‐derived hepatocyte‐like cells (hiPSCs‐Heps) efficiently entered into the differentiation phase (G1) and gradually took a polygonal shape, a typical shape of hepatocytes. The expression of hepatic‐associated genes (albumin, TAT, Cytokeratin19, and Cyp7A1), albumin and urea secretion in hiPSCs‐Heps cultured into DWJS was significantly higher than those cultured in the culture plates (2D). Altogether, our results suggest that DWJS could provide a proper microenvironment that efficiently promotes hepatic differentiation of hiPSCs.

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“…Decellularized liver scaffold‐based engineering approach holds promises for developing functional liver surrogates . The resulting decellularized liver scaffold preserves a three‐dimensional (3D) matrix architecture that closely mimics the native liver and retains a plethora of matrix‐associated proteins as well as endogenous growth factors …”

Section: Introductionmentioning

confidence: 99%

“…A variety of cell types have been examined for liver scaffold recellularization, including immortalized cells, adult primary cells, fetal cells, mesenchymal stem cells and induced pluripotent stem cells . Many studies have shown that recellularized liver scaffolds perform hepatic specific functions in vitro .…”

Section: Introductionmentioning

confidence: 99%

Vasculature reconstruction of decellularized liver scaffolds via gelatin‐based re‐endothelialization

Meng

Almohanna

Altuhami

et al. 2018

J Biomedical Materials Res

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Decellularized liver scaffolds based liver engineering is a promising approach toward developing functional liver surrogates. However, a major obstacle to long‐term transplantation is the hemocompatibility of the bioengineered liver surrogates. One approach to improve the hemocompatibility of engineered liver surrogates is re‐endothelialization. In the current study, immortalized endothelial cells were perfused for re‐endothelialization of decellularized rat liver scaffolds. When compared to the media‐based perfusion approach, gelatin hydrogels‐based perfusion significantly increased the number of cells that were retained in the decellularized liver scaffolds and the vascular lumen coverage ratio. Endothelial cells were lining along the vasculatures of the decellularized liver scaffolds and actively proliferating. Re‐endothelialization improved the blood retention ability of the liver scaffold vasculatures. Doppler ultrasound detected active blood flows within the re‐endothelialized liver scaffold transplants 8 days post‐transplantation. Our results strengthened the feasibility of developing bioengineered liver surrogates utilizing decellularized liver scaffolds. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 392–402, 2019.

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Decellularized Liver Extracellular Matrix as Promising Tools for Transplantable Bioengineered Liver Promotes Hepatic Lineage Commitments of Induced Pluripotent Stem Cells

Cited by 97 publications

References 55 publications

Extracellular matrix hydrogels from decellularized tissues: Structure and function

Extracellular matrix hydrogels from decellularized tissues: Structure and function

Decellularized Wharton's jelly extracellular matrix as a promising scaffold for promoting hepatic differentiation of human induced pluripotent stem cells

Vasculature reconstruction of decellularized liver scaffolds via gelatin‐based re‐endothelialization

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