Decellularized kidney scaffold alters the healing response in chronic renal failure

Abstract

Decellularized (DC) kidney scaffold shows great potential for renal recovering. Our study explored the effect of the DC kidney scaffolds treating on chronic renal failure (CRF) through grafting them on 5/6 nephrectomized (5/6 Nx) rat kidneys compared with gelatin sponges covered the incision edges. Blood urea nitrogen and angiotensin II were significantly lower in most time in scaffold‐grafted groups. Remnant kidney tumor necrosis factor‐α and fibroblast growth factor in scaffold‐grafted groups significantly reduced in majority of time points compared with controls. But platelet‐derived growth factor‐BB showed a different varied tendency, first higher in scaffold groups on week 2, 4, 6, but lower on week 8, finally no difference on week 12 compared with gelatin‐sponge groups. In addition, the index of glomerular sclerosis was significantly lesser in scaffold‐grafted groups, and, the accumulation of collagen III and collagen IV decreased in scaffold‐grafted groups on week 6, 8, 12 compared with gelatin‐sponge groups. Moreover, DC scaffolds enhanced the expression of CD133 on week 2, 6, 8, 12. In conclusion, DC kidney scaffold altered the healing response after 5/6 nephrectomy and ameliorated renal injury to some degree. Therefore, DC kidney scaffold could be a promising therapeutic method on CRF.

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