Decellularized human periodontal ligament for periodontium regeneration

Son, Hyoju; Jeon, Mijeong; Choi, Hyung Jun; Lee, Hyo Seol; Kim, Ik Hwan; Kang, Chung-Min; Song, Je Seon

doi:10.1371/journal.pone.0221236

Cited by 26 publications

(19 citation statements)

References 36 publications

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“…Synthetic scaffold using CAD/CAM technologies, laserassisted bioprinting technologies, and a scaffold using decellularized organs are being studied to improve the effectiveness of tissue regeneration [19,20] including dental tissue regeneration [21]. Among the available dental scaffolds, decellularized tissues, decellularized human tooth, and tooth bud scaffolds were studied as a promising dental tissue regeneration scaffolds in current studies [22][23][24]. Moreover, the results of dental tissue regeneration are affected by the delivery of growth factors.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Evaluation of Decellularized Human Tooth Scaffold for Dental Tissue Regeneration

et al. 2021

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Conventional root canal treatment may result in loss of tooth vitality, which can lead to unfavorable treatment outcomes. Notably, a ceased tooth development of immature permanent teeth with open apices, regeneration of periodontal ligaments (PDL), and pulp is highly expected healing process. For regeneration, the scaffold is one of the critical components that carry biological benefits. Therefore, this study evaluated a decellularized human tooth as a scaffold for the PDL and pulp tissue regeneration. A tooth scaffold was fabricated using an effective decellularization method as reported in previous studies. PDL stem cells (PDLSCs) and dental pulp stem cells (DPSCs) obtained from human permanent teeth were inoculated onto decellularized scaffolds, then cultured to transplant into immunosuppressed mouse. After 9 weeks, PDLSCs and DPSCs that were inoculated onto decellularized tooth scaffolds and cultured in an in vivo demonstrated successful differentiation. In PDLSCs, a regeneration of the cementum/PDL complex could be expected. In DPSCs, the expression of genes related to revascularization and the hard tissue regeneration showed the possibility of pulp regeneration. This study suggested that the potential possible application of decellularized human tooth could be a scaffold in regeneration PDL and pulp tissue along with PDLSCs and DPSCs, respectively, as a novel treatment method.

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Section: Introductionmentioning

confidence: 99%

In Vivo Evaluation of Decellularized Human Tooth Scaffold for Dental Tissue Regeneration

et al. 2021

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“…Human PDL cells (hPDLCs) are heterogeneous cells sharing the features of mesenchymal stem cells (MSCs) and viewed as promising stem cells for periodontal regeneration therapy ( Tomokiyo et al, 2019 ; Trubiani et al, 2019 ). hPDLCs can differentiate into osteoblasts, adipocytes, and cementoblasts, contributing to the physiological healing of cementum-periodontal ligament complex and alveolar bone ( Son et al, 2019 ). Osteogenic differentiation enables hPDLCs to be a potential target for the promotion of functional restoration of periodontal tissues ( Kato et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

MiR-143-3p Inhibits Osteogenic Differentiation of Human Periodontal Ligament Cells by Targeting KLF5 and Inactivating the Wnt/β-Catenin Pathway

Wangzhou

Lai

et al. 2021

Front. Physiol.

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Human periodontal ligament cells (hPDLCs) play a vital role in cell regeneration and tissue repair with multi-directional differentiation potential. microRNAs (miRs) are implicated in the osteogenesis of hPDLCs. This study explored the mechanism of miR-143-3p in osteogenesis of hPDLCs. Osteogenic differentiation of isolated hPDLCs was induced. KLF5 expression during osteogenic differentiation of hPDLCs was detected and then silenced in hPDLCs. Binding relationship between KLF5 and miR-143-3p was predicted and verified. hPDLCs were treated with miR-143-3p mimic or overexpressing KLF5, and then osteogenic specific markers and mineralized nodules were measured. The key factors of the Wnt/β-catenin pathway during osteogenesis of hPDLCs were measured. KLF5 expression was upregulated during osteogenesis of hPDLCs. KLF5 silencing or miR-143-3p mimic reduced osteogenic specific markers and mineralized nodules. Overexpression of KLF5 could reverse the inhibitory effect of miR-143-3p on osteogenic differentiation. miR-143-3p mimic and KLF5 silencing inactivated the Wnt/β-catenin pathway. Activation of the Wnt/β-catenin pathway reversed the repression effect of miR-143-3p mimic on osteogenesis of hPDLCs. In conclusion, miR-143-3p inhibited osteogenic differentiation of hPDLCs by targeting KLF5 and inactivating the Wnt/β-catenin pathway.

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Section: Introductionmentioning

confidence: 99%

“…An ideal scaffold is crucial for regenerating PDL, and microfibrous scaffolds mimicking native PDL to guide PDL formation, so-called ‘fiber-guiding scaffolds’ ( Figure 1 ), created by decellularizing tooth slices or 3D-printed (3DP) polymeric scaffolds, have been proposed as viable options to assist in the optimal reconstruction of PDL microarchitecture [ 8 , 9 ]. Decellularized tooth slices were reported to support the repopulation and differentiation of PDL cells [ 9 ]. However, the success of decellularization might be affected by the tissue conditions and decellularization protocols, and incomplete decellularization might induce an immune response that negatively influences the treatment outcome [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament Reconstruction

Lin

Chao

Tai

et al. 2021

IJMS

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Reconstruction of the periodontal ligament (PDL) to fulfill functional requirement remains a challenge. This study sought to develop a biomimetic microfibrous system capable of withstanding the functional load to assist PDL regeneration. Collagen-based straight and waveform microfibers to guide PDL cell growth were prepared using an extrusion-based bioprinter, and a laminar flow-based bioreactor was used to generate fluidic shear stress. PDL cells were seeded on the respective microfibers with 0 or 6 dynes/cm2 fluidic shear stress for 1–4 h. The viability, morphology, adhesion pattern, and gene expression levels of PDL cells were assessed. The results revealed that upon bioprinting optimization, collagen-based microfibers were successfully fabricated. The straight microfibers were 189.9 ± 11.44 μm wide and the waveform microfibers were 235.9 ± 11.22 μm wide. Under 6 dynes/cm2 shear stress, PDL cells were successfully seeded, and cytoskeleton expansion, adhesion, and viability were greater. Cyclin D, E-cadherin, and periostin were upregulated on the waveform microfibers. In conclusion, 3D-printed collagen-based waveform microfibers preserved PDL cell viability and exhibited an enhanced tendency to promote healing and regeneration under shear stress. This approach is promising for the development of a guiding scaffold for PDL regeneration.

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Decellularized human periodontal ligament for periodontium regeneration

Cited by 26 publications

References 36 publications

In Vivo Evaluation of Decellularized Human Tooth Scaffold for Dental Tissue Regeneration

In Vivo Evaluation of Decellularized Human Tooth Scaffold for Dental Tissue Regeneration

MiR-143-3p Inhibits Osteogenic Differentiation of Human Periodontal Ligament Cells by Targeting KLF5 and Inactivating the Wnt/β-Catenin Pathway

3D-Printed Collagen-Based Waveform Microfibrous Scaffold for Periodontal Ligament Reconstruction

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