Decellularized bovine small intestinal submucosa-PCL/hydroxyapatite-based multilayer composite scaffold for hard tissue repair

Sustained delivery of PlGF-2123-144*-fused BMP2-related peptide P28 from small intestinal submucosa/polylactic acid scaffold material for bone tissue regeneration

“…Previous studies have also mixed biodegradable polymers with natural polymer materials. 16,36 BMP-2 related peptides represent an effective method to improve the disadvantages associated with the clinical treatment of bone defects using BMP-2. The ability to combine a small peptide with various scaffold materials can be further enhanced through the modication of the structure with small molecule peptides, which both increases the amount of peptide drug retained on the scaffolds and reduces the release rate.…”

Scaffolding Strategies for Tissue Engineering and Regenerative Medicine Applications

“…The combination of Sr with Zn led to a significant influence on cell proliferation and osteogenesis in comparison to the single ions. Several studies have been reported using dECM-based scaffolds for TERM [17,18,19,106,107]. In fact, those types of scaffolds confer an ideal microenvironment, with instructive biological molecules and reduced immuno responses [108].…”

“…The dECM-based scaffolds improved rat bone marrow stem cells (BMSC) proliferation when compared with scaffolds without dECM. In another study, Parmaksiz et al [19] developed a multilayer scaffold of decellularized bovine small intestinal submucosa (bSIS) layers, together with HAp microparticles and PCL, with potential for bone TE. For that, bSIS layers were stacked with PCL solutions that acted as a glue, in order to improve the mechanical properties.…”

“…In fact, dECM preserves the native tissue composition, not only in terms of structural proteins as collagen, but also preserves growth factors and cytokines, which can improve cell growth and viability, and tissue repair and remodeling [15]. Further, dECM has been obtained by means of employing different processing methodologies and from a diversity of tissues, such as bone, cartilage, meniscus, tendons, skin and adipose tissue, urinary bladder, small intestinal submucosa, liver, and brain [16,17,18,19,20,21]. On the other hand, the lack of mechanical properties of those biomaterials can be overcome by means of using synthetic-based polymers or combining them with inorganic and ceramic materials to form composite structures with superior strength, osteoconductivity, and bioresorbability [22,23,24].…”

Synthetic Biodegradable Aliphatic Polyester Nanocomposites Reinforced with Nanohydroxyapatite and/or Graphene Oxide for Bone Tissue Engineering Applications

“…Electrospun nanofibers with interconnected porous structure and large surface area show morphological similarities to the natural ECM [140,141]. By adding nHA to biodegradable polymers, the resultant fibrous scaffolds favor cell adhesion, growth and differentiation [81,142,143,144]. However, the pores of electrospun scaffolds are rather small, typically about 200–800 nm and below 5 µm, thus restricting bone cell infiltration and vascular ingrowth [145].…”