Decellularized bone marrow reveals a novel niche protein that modulate adhesion

Lund, Troy C.; Kramer, Ashley C.; Taisto, Mandy; Blake, Amanda L.; Lehrke, Michael Jonathan

doi:10.1016/j.exphem.2017.06.177

Cited by 1 publication

(3 citation statements)

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“…To date, the simplest models for examining biological behavior of MSCs in response to microenvironmental factors are conduced by direct exposition to soluble factors (Celil and Campbell, 2005;Indrawattana et al, 2004;Kratchmarova et al, 2005;Luo et al, 2010) and conditioned media from either other cell type cultures (Chowdhury et al, 2015;Menon et al, 2007;Siciliano et al, 2015) or from tissue extracts (Chen et al, 2002).…”

Section: Paracrine Factorsmentioning

confidence: 99%

“…Overall, the co-culture systems can be set-up either by direct co-culture of both cell types or using compartmented systems, such as trans-wells or microfluidic chambers, to study solely the paracrine crosstalk and not the cell-cell signaling pathways that may occur. Many studies have explored this approach by co-culturing MSCs with mature cells in direct (Aguirre et al, 2010;Ball et al, 2004;Csaki et al, 2009;Deng et al, 2008;Strassburg et al, 2010;Takigawa et al, 2017;Wang et al, 2007) and indirect contact (Li et al, 2011;Luo et al, 2009;Wei et al, 2010). When comparing cultures of MSCs and osteoblasts alone with MSC/osteoblast co-cultures, for example, an increase in calcification over time is observed in co-culture.…”

Section: Co-culturesmentioning

confidence: 99%

“…Although a variety of different materials and composites are available, to achieve a physiologically relevant protein and structural complexity, whole organ or tissue decellularization techniques are investigated. These natural scaffolds preserve the complex biochemical and biomechanical ultrastructure of the native tissue and can be recellularized to generate a new functional tissue or organ (Crapo et al, 2011;Lund et al, 2017). Particularly, decellularized bone is used as a scaffold for bone www.ecmjournal.org tissue engineering or bone in vitro modelling due to its 3D porous structure and its natural biochemical component arrangement, providing osteoinductive properties that are not fully resembled by synthetic polymers or hydrogels (Nyberg et al, 2017).…”

Section: Decellularized Tissuementioning

confidence: 99%

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Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro

Pereira¹,

Trivanović²,

Herrmann³

2019

eCM

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Mesenchymal stem/stromal cells (MSCs) are an essential element of most modern tissue engineering and regenerative medicine approaches due to their multipotency and immunoregulatory functions. Despite the prospective value of MSCs for the clinics, the stem cells community is questioning their developmental origin, in vivo localization, identification, and regenerative potential after several years of far-reaching research in the field. Although several major progresses have been made in mimicking the complexity of the MSC niche in vitro, there is need for comprehensive studies of fundamental mechanisms triggered by microenvironmental cues before moving to regenerative medicine cell therapy applications. The present comprehensive review extensively discusses the microenvironmental cues that influence MSC phenotype and function in health and disease-including cellular, chemical and physical interactions. The most recent and relevant illustrative examples of novel bioengineering approaches to mimic biological, chemical, and mechanical microenvironmental signals present in the native MSC niche are summarized, with special emphasis on the forefront techniques to achieve biochemical complexity and dynamic cultures. In particular, the skeletal MSC niche and applications focusing on the bone regenerative potential of MSC are addressed. The aim of the review was to recognize the limitations of the current MSC niche in vitro models and to identify potential opportunities to fill the bridge between fundamental science and clinical application of MSCs.

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