Decellularization of the Porcine Ear Generates a Biocompatible, Nonimmunogenic Extracellular Matrix Platform for Face Subunit Bioengineering

Duisit, Jérôme; Orlando, Giuseppe; Debluts, Donovan; Maistriaux, Louis; Xhema, Daela; Bisthoven, Yann-Alex Janssens de; Galli, Cesare; Peloso, Andréa; Behets, Catherine; Lengelé, Benoît; Gianello, Pierre

doi:10.1097/sla.0000000000002181

Cited by 42 publications

(49 citation statements)

References 36 publications

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“…Despite robust initial recellularization, subsequent growth, and proliferation observed in all cell types with no obvious differences between cells seeded onto wild‐type versus α‐Gal KO lungs, these findings certainly raise concerns for clinical translation. A recent study on face subunit bioengineering, however, showed that ECM scaffolds obtained from the porcine ear may be nonimmunogenic . In fact, when implanted in discordant pigs, such scaffolds did not trigger any specific anti‐swine leukocyte antigen (SLA) humoral response, as demonstrated by a lack of donor‐specific antibodies.…”

Section: Scaffolds From Xenogenic and Allogeneic Organsmentioning

confidence: 99%

Regenerative immunology: the immunological reaction to biomaterials

et al. 2017

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Regenerative medicine promises to meet two of the most urgent needs of modern organ transplantation, namely immunosuppression-free transplantation and an inexhaustible source of organs. Ideally, bioengineered organs would be manufactured from a patient's own biomaterials-both cells and the supporting scaffolding materials in which cells would be embedded and allowed to mature to eventually regenerate the organ in question. While some groups are focusing on the feasibility of this approach, few are focusing on the immunogenicity of the scaffolds that are being developed for organ bioengineering purposes. This review will succinctly discuss progress in the understanding of immunological characteristics and behavior of different scaffolds currently under development, with emphasis on the extracellular matrix scaffolds obtained decellularized animal or human organs which seem to provide the ideal template for bioengineering purposes.

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Section: Scaffolds From Xenogenic and Allogeneic Organsmentioning

confidence: 99%

Regenerative immunology: the immunological reaction to biomaterials

et al. 2017

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“…10,11 Decellularized ECM therapies continue to be investigated through clinical trials, addressing a multitude of application anatomic sites, including the trachea, heart valves, eye, and bladder among many others. 12 Furthermore, complex structural ECM scaffolds generated from perfusion decellularization, such as heart, 13,14 lung, 15 kidney, 16 or face, 17 are within the foreseeable pipeline for future clinical translation.…”

Section: Introductionmentioning

confidence: 99%

Design and Fabrication of an Automatable, 3D Printed Perfusion Device for Tissue Infusion and Perfusion Engineering

Schilling

Lamberti

Snowden

et al. 2020

Tissue Engineering Part A

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“…They showed that well-decellularized scaffolds are poorly, if not at all, immunogenic and possibly tolerogenic, while non-well-decellularized tissues are prone to inflammatory response and degradation or encapsulation [24-27]. …”

Section: Discussionmentioning

confidence: 99%

Enhanced Vascular Biocompatibility and Remodeling of Decellularized and Secured Xenogeneic/Allogeneic Matrices in a Porcine Model

et al. 2018

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Background/Purpose: Calcifications and absence of growth potential are the major drawbacks of glutaraldehyde-treated prosthesis. Decellularized and secured xeno-/allogeneic matrices were assessed in a preclinical porcine model for biocompatibility and vascular remodeling in comparison to glutaraldehyde-fixed bovine pericardium (GBP; control). Methods: Native human (fascia lata, pericardium) and porcine tissues (peritoneum) were used and treated. In vitro, biopsies were performed before and after treatment to assess decellularization (hematoxylin and eosin/DAPI). In vivo, each decellularized and control tissue sample was implanted subcutaneously in 4 mini-pigs. In addition, 9 mini-pigs received a patch or a tubularized prosthesis interposition on the carotid artery or abdominal aorta of decellularized (D) human fascia lata (DHFL; n = 4), human pericardium (DHP; n = 9), porcine peritoneum (DPPt; n = 7), and control tissue (GBP: n = 3). Arteries were harvested after 1 month and subcutaneous samples after 15–30 days. Tissues were processed for hematoxylin and eosin/von Kossa staining and immunohistochemistry for CD31, alpha-smooth muscle actin, CD3, and CD68. Histomorphometry was achieved by point counting. Results: A 95% decellularization was confirmed for DHP and DPPt, and to a lower degree for DHFL. In the subcutaneous protocol, CD3 infiltration was significantly higher at day 30 in GBP and DHFL, and CD68 infiltration was significantly higher for GBP (p < 0.05). In intravascular study, no deaths, aneurysms, or pseudoaneurysms were observed. Inflammatory reaction was significantly higher for DHFL and GBP (p < 0.05), while it was lower and comparable for DHP/DPPt. DHP and DPPt showed deeper recellularization, and a new arterial wall was characterized. Conclusions: In a preclinical model, DPPt and DHP offered better results than conventional commercialized GBP for biocompatibility and vascular remodeling.

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Decellularization of the Porcine Ear Generates a Biocompatible, Nonimmunogenic Extracellular Matrix Platform for Face Subunit Bioengineering

Abstract: Acellular scaffolds were produced from the porcine ear, and may be a valuable platform to treat facial deformities using regenerative medicine approaches.

Cited by 42 publications

References 36 publications

Regenerative immunology: the immunological reaction to biomaterials

Regenerative immunology: the immunological reaction to biomaterials

Design and Fabrication of an Automatable, 3D Printed Perfusion Device for Tissue Infusion and Perfusion Engineering

Enhanced Vascular Biocompatibility and Remodeling of Decellularized and Secured Xenogeneic/Allogeneic Matrices in a Porcine Model

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