Decellularization and Recellularization of Rabbit Kidney Using Adipose-Derived Mesenchymal Stem Cells for Renal Tissue Engineering

Sabetkish, Shabnam; Sabetkish, Nastaran; Ekhtiari, Masoumeh; Jobani, Bahareh Mohammadi; Kajbafzadeh, Abdol‐Mohammad

doi:10.1007/s40883-020-00177-9

Cited by 9 publications

(8 citation statements)

References 29 publications

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“…During these experiments, it was observed that ASCs were able to engraft the glomerular, tubular, and vascular areas of the scaffold, eventually trans-differentiating into RTECs and endothelial cells. The authors postulated that the attachment to the scaffold was mediated by the stromal cell-derived factor 1α (SDF-1

) [ 66 ]. It remains unclear whether ASCs could trans-differentiate into other renal cell types or build up a proper tridimensional structure.…”

Section: Mechanisms Of Actionmentioning

confidence: 99%

Adipose-Derived Stem/Stromal Cells in Kidney Transplantation: Status Quo and Future Perspectives

Storti

Favi

Albanesi

et al. 2021

IJMS

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Kidney transplantation (KT) is the gold standard treatment of end-stage renal disease. Despite progressive advances in organ preservation, surgical technique, intensive care, and immunosuppression, long-term allograft survival has not significantly improved. Among the many peri-operative complications that can jeopardize transplant outcomes, ischemia–reperfusion injury (IRI) deserves special consideration as it is associated with delayed graft function, acute rejection, and premature transplant loss. Over the years, several strategies have been proposed to mitigate the impact of IRI and favor tolerance, with rather disappointing results. There is mounting evidence that adipose stem/stromal cells (ASCs) possess specific characteristics that could help prevent, reduce, or reverse IRI. Immunomodulating and tolerogenic properties have also been suggested, thus leading to the development of ASC-based prophylactic and therapeutic strategies in pre-clinical and clinical models of renal IRI and allograft rejection. ASCs are copious, easy to harvest, and readily expandable in culture. Furthermore, ASCs can secrete extracellular vesicles (EV) which may act as powerful mediators of tissue repair and tolerance. In the present review, we discuss the current knowledge on the mechanisms of action and therapeutic opportunities offered by ASCs and ASC-derived EVs in the KT setting. Most relevant pre-clinical and clinical studies as well as actual limitations and future perspective are highlighted.

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) [ 66 ]. It remains unclear whether ASCs could trans-differentiate into other renal cell types or build up a proper tridimensional structure.…”

Section: Mechanisms Of Actionmentioning

confidence: 99%

Adipose-Derived Stem/Stromal Cells in Kidney Transplantation: Status Quo and Future Perspectives

Storti

Favi

Albanesi

et al. 2021

IJMS

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“…Organ engineering has successfully integrated a functional tissue-engineered cardiac muscle graft to improve myocardial function [5][6][7]. Similar efforts have also been reported in other organs, such as the liver, kidney, lung, pancreas [8][9][10][11][12][13][14]. It is recently reported that a cartilage like tissue has been successfully engineered using human decellularized extracellular matrix (hECM) scaffolds seeded with human adipose stem cells (hASCs) [15].…”

Section: Introductionmentioning

confidence: 96%

Recellularization of Native Tissue Derived Acellular Scaffolds with Mesenchymal Stem Cells

Ahmed

Saleh

2021

Cells

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The functionalization of decellularized scaffolds is still challenging because of the recellularization-related limitations, including the finding of the most optimal kind of cell(s) and the best way to control their distribution within the scaffolds to generate native mimicking tissues. That is why researchers have been encouraged to study stem cells, in particular, mesenchymal stem cells (MSCs), as alternative cells to repopulate and functionalize the scaffolds properly. MSCs could be obtained from various sources and have therapeutic effects on a wide range of inflammatory/degenerative diseases. Therefore, in this mini-review, we will discuss the benefits using of MSCs for recellularization, the factors affecting their efficiency, and the drawbacks that may need to be overcome to generate bioengineered transplantable organs.

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“…Current studies have outlined vascular events that limit whole organ decellularization and recellularization techniques for long-term transplantation. Several microscopic and macroscopic approaches have examined the whole kidney scaffold structure and function in various settings [18][19][20][21][22][23][24][25][26]. Such investigations have proved that the decellularization process successfully removes remnant native cellular/tissue components and decellularizing agents while maintaining the vascular and ECM architectures in various large and small animal models.…”

Section: Introductionmentioning

confidence: 99%

Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy

Corridon

2023

Preprint

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Organ decellularization creates cell-free, collagen-based extracellular matrices that can be used as scaffolds for tissue engineering applications. This technique has recently gained much attention, yet adequate scaffold repopulation and implantation remain a challenge. Specifically, there still needs to be a greater understanding of scaffold responses post-transplantation and ways we can improve scaffold durability to withstand the in vivo environment. Recent studies have outlined vascular events that limit organ decellularization/recellularization scaffold viability for long-term transplantation. However, these insights have relied on in vitro/in vivo approaches that need enhanced spatial and temporal resolutions to investigate such issues at the microvascular level. This study uses intravital microscopy to gain instant feedback on their structure, function, and deformation dynamics. Thus, the objective of this study was to capture the effects of in vivo blood flow on the decellularized glomerulus, peritubular capillaries, and tubules after autologous and allogeneic orthotopic transplantation into rats. Large molecular weight dextran molecules label the vasculature. They revealed substantial degrees of translocation from glomerular and peritubular capillary tracks to the decellularized tubular epithelium and lumen as early as 12 hours after transplantation, providing real-time evidence of the increases in microvascular permeability. Macromolecular extravasation persisted for a week, during which the decellularized microarchitecture was significantly and comparably compromised and thrombosed in both autologous and allogeneic approaches. These results indicate that in vivo multiphoton microscopy is a powerful approach for studying scaffold viability and identifying ways to promote scaffold longevity and vasculogenesis in bioartificial organs.

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Decellularization and Recellularization of Rabbit Kidney Using Adipose-Derived Mesenchymal Stem Cells for Renal Tissue Engineering

Cited by 9 publications

References 29 publications

Adipose-Derived Stem/Stromal Cells in Kidney Transplantation: Status Quo and Future Perspectives

Adipose-Derived Stem/Stromal Cells in Kidney Transplantation: Status Quo and Future Perspectives

Recellularization of Native Tissue Derived Acellular Scaffolds with Mesenchymal Stem Cells

Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy

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