Background: Awareness and understanding of organ donation after brain death can significantly influence an individuals' perceptions of the issue. Objectives: The aim of this study was to determine the current level of awareness and perception of the Slovenian public regarding organ donation after brain death. Methods: A cross-sectional study was conducted using a questionnaire to collect data from a sample of 784 individuals. Inferential statistics were conducted using IBM SPSS version 25.Results: The research sample of the studied Slovenian population demonstrated support for organ donation after brain death, despite a relatively low level of awareness of the topic. Significant demographic differences emerged in respondents' perceptions of organ donation. Female respondents, individuals with higher levels of educational attainment, and those working in health and social care exhibited more positive attitudes. In contrast, the age group of 50 years and above obtained the lowest scores. Statistically significant differences in awareness levels were only observed among different work sectors, with individuals working in health and social care showing higher levels of awareness. A positive yet weak correlation was found between the awareness and perceptions regarding the topic. Conclusion: Raising public awareness is essential for promoting organ donation. This can be achieved through the dissemination of information on the subject by experienced professionals.