Decay‐accelerating factor 1 deficiency exacerbates <i>Trypanosoma cruzi</i>‐induced murine chronic myositis

Solana, María Elisa; Ferrer, María Florencia; Novoa, María Mercedes; Song, Wen‐Chao; Gómez, Ricardo Martín

doi:10.1002/mus.23347

Cited by 11 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In comparison with WT, in previous studies, Daf1 −/− mice have shown higher acute viral load in murine CMV [32] but lower viral load in acute and chronic LCMV infection [33] or bacteremia after Pneumococcal pneumonia infection [34]. Reduced parasite load was also found in chronic Trypanosome cruzi -infected mice [35]. It is not clear why Daf1 −/− mice showed an early drop in survival and a higher bacterial burden at the start of the leptospiruric phase.…”

Section: Discussionmentioning

confidence: 88%

“…Standard cDNA samples with 10-fold serial dilutions were used for PCR efficiency calculations. Cycle threshold (Ct) values were obtained for each individual reaction, and the Ct of the host-expressed β-actin was subtracted to obtain pro-collagen type I, SMA, Gal-3, TGF-β1, IL-4, IL-10, IL-12, IL-13, IL-17 and IFN-γ normalized values, respectively [35], [43]. To obtain bacterial burden values, 16SDNA bacterial gene was amplified and the number of bacteria was referred to that of the host cells [35].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Decay-Accelerating Factor 1 Deficiency Exacerbates Leptospiral-Induced Murine Chronic Nephritis and Renal Fibrosis

et al. 2014

Self Cite

View full text Add to dashboard Cite

Leptospirosis is a global zoonosis caused by pathogenic Leptospira, which can colonize the proximal renal tubules and persist for long periods in the kidneys of infected hosts. Here, we characterized the infection of C57BL/6J wild-type and Daf1−/− mice, which have an enhanced host response, with a virulent Leptospira interrogans strain at 14 days post-infection, its persistence in the kidney, and its link to kidney fibrosis at 90 days post-infection. We found that Leptospira interrogans can induce acute moderate nephritis in wild-type mice and is able to persist in some animals, inducing fibrosis in the absence of mortality. In contrast, Daf1−/− mice showed acute mortality, with a higher bacterial burden. At the chronic stage, Daf1−/− mice showed greater inflammation and fibrosis than at 14 days post-infection and higher levels at all times than the wild-type counterpart. Compared with uninfected mice, infected wild-type mice showed higher levels of IL-4, IL-10 and IL-13, with similar levels of α-smooth muscle actin, galectin-3, TGF-β1, IL-17, IFN-γ, and lower IL-12 levels at 90 days post-infection. In contrast, fibrosis in Daf1−/− mice was accompanied by high expression of α-smooth muscle actin, galectin-3, IL-10, IL-13, and IFN-γ, similar levels of TGF-β1, IL-12, and IL-17 and lower IL-4 levels. This study demonstrates the link between Leptospira-induced murine chronic nephritis with renal fibrosis and shows a protective role of Daf1.

show abstract

Section: Discussionmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Decay-Accelerating Factor 1 Deficiency Exacerbates Leptospiral-Induced Murine Chronic Nephritis and Renal Fibrosis

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Evaluation of the tissue specimens was performed by light microscopy by a researcher blinded to the treatment assignment. Presence of amastigote nests was recorded and the degree of myocardial and skeletal inflammation was scored as described previously [15,16].…”

Section: Tissue Sample Preparation and Histological Evaluationmentioning

confidence: 99%

Molecular and biological characterization of a highly pathogenic Trypanosoma cruzi strain isolated from a patient with congenital infection

Gulin

Bisio

Rocco

et al. 2018

Experimental Parasitology

View full text Add to dashboard Cite

Although many Trypanosoma cruzi (T. cruzi) strains isolated from a wide range of hosts have been characterized, there is a lack of information about biological features from vertically transmitted strains. We describe the molecular and biological characteristics of the T. cruzi VD strain isolated from a congenital Chagas disease patient. The VD strain was typified as DTU TcVI; in vitro sensitivity to nifurtimox (NFX) and beznidazole (BZ) were 2.88 μM and 6.19 μM respectively, while inhibitory concentrations for intracellular amastigotes were 0.24 μM for BZ, and 0.66 μM for NFX. Biological behavior of VD strain was studied in a mouse model of acute infection, resulting in high levels of parasitemia and mortality with a rapid clearence of bloodstream trypomastigotes when treated with BZ or NFX, preventing mortality and reducing parasitic load and intensity of inflammatory infiltrate in skeletal and cardiac muscle. Treatment-induced parasitological cure, evaluated after immunossupression were 41% and 35% for BZ and NFX treatment respectively, suggesting a partial response to these drugs in elimination of parasite burden. This exhaustive characterization of this T. cruzi strain provides the basis for inclusion of this strain in a panel of reference strains for drug screening and adds a new valuable tool for the study of experimental T. cruzi infection.

show abstract

“…The same authors found calcifications in the skeletal muscle studied, but they studied the rectus abdominis muscle. A recent study reports the presence of skeletal muscle fibers undergoing calcification and necrosis as a consequence of T. cruzi II infection in WT and Daf1-deficient mice [37]. There is another report that superficially mentions muscular calcification as a consequence of T. cruzi infection, but that study was achieved using also a genotype II strain [38].…”

Section: Discussionmentioning

confidence: 99%

Exacerbated Skeletal Muscle Inflammation and Calcification in the Acute Phase of Infection by MexicanTrypanosoma cruziDTUI Strain

Vizcaíno-Castillo

Jiménez-Marín

Espinoza

2014

BioMed Research International

View full text Add to dashboard Cite

A murine model was used to study the histopathological aspects and cytokine expression levels in skeletal muscle provoked by the infection with Mexican TcI strains. BALB/c mice were inoculated with the virulent Querétaro strain and the nonvirulent Ninoa strain. Parasite numbers were counted in blood and skeletal muscle at different times post-infection, and real time-PCR expression levels of the cytokines IL-12, IL-4, IL-10, IFN-γ, and TNF-α were evaluated. In the acute phase of infection, a high parasitic load, both in blood and skeletal muscle, was detected. The histopathological analyses showed an exacerbated inflammation and granulomatous-like infiltrate with the Querétaro strain. Interestingly, extensive calcification areas were observed in the skeletal muscle surrounded by inflammatory infiltrates. TNF-α and IL-10 expression exhibited a significant increase at the peak of infection. In summary, Querétaro strain, a Mexican TcI strain, is virulent enough to induce high inflammation and calcification in skeletal muscle of the hind limbs, which could be related to high expression levels of TNF-α.

show abstract

Decay‐accelerating factor 1 deficiency exacerbates Trypanosoma cruzi‐induced murine chronic myositis

Cited by 11 publications

References 36 publications

Decay-Accelerating Factor 1 Deficiency Exacerbates Leptospiral-Induced Murine Chronic Nephritis and Renal Fibrosis

Decay-Accelerating Factor 1 Deficiency Exacerbates Leptospiral-Induced Murine Chronic Nephritis and Renal Fibrosis

Molecular and biological characterization of a highly pathogenic Trypanosoma cruzi strain isolated from a patient with congenital infection

Exacerbated Skeletal Muscle Inflammation and Calcification in the Acute Phase of Infection by MexicanTrypanosoma cruziDTUI Strain

Contact Info

Product

Resources

About