Debris Remaining in the Apical Third of Root Canals after Chemomechanical Preparation by Using Sodium Hypochlorite and Glyde: An In Vivo Study

Cruz, Álvaro; Vera, Jorge; Gascón, Gerardo; Palafox‐Sánchez, Claudia Azucena; Amezcua, Octavio; Mercado, Gabriela

doi:10.1016/j.joen.2014.05.013

Cited by 17 publications

(17 citation statements)

References 18 publications

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“…, Cruz et al . ), previous studies suggest that the use of liquid or gel‐like chelating agents during instrumentation may not significantly affect root canal transportation (Whitbeck et al . ).…”

Section: Discussionmentioning

confidence: 98%

“…Chelating agent in gel was utilized during canal scouting and initial glide path, and 10% EDTA liquid solution alternated with 5% NaOCl was used as irrigant during completion of the glide path and shaping. Regardless of whether liquid chelating agents more effectively remove smear layer (H€ ulsmann et al 2002, Lim et al 2003, Cruz et al 2014, previous studies suggest that the use of liquid or gel-like chelating agents during instrumentation may not significantly affect root canal transportation (Whitbeck et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Following access cavity preparation, canal scouting and initial glide path were performed in all specimens with a size 10 K-file at WL using Glyde TM (Dentsply Maillefer, Ballaigues, Switzerland) as lubricating agent (0.80 mg) (Cruz et al 2014). WL was established under 109 magnification (OPMI Pro Ergo, Carl Zeiss, Oberkochen, Germany) when the tip of the instrument was visible at the apical foramen and then subtracting 0.5 mm from this length.…”

Section: Specimen Preparationmentioning

confidence: 99%

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Micro‐CT evaluation of several glide path techniques and ProTaper Next shaping outcomes in maxillary first molar curved canals

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Specimen Preparationmentioning

confidence: 99%

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Micro‐CT evaluation of several glide path techniques and ProTaper Next shaping outcomes in maxillary first molar curved canals

et al. 2016

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“…performed, the irrigating solution must present biocompatibility, in order to keep the integrity of the periapical structures, not promoting irritation of the remaining pulp tissue and allowing repair [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Ethanolic Extract of Psidium cattleianum as Intracanal Irrigation: An In vitro Study of Cell Viability

Martins¹

2017

J. Dent. Sci. Ther

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The aim of this study was to evaluate the ethanolic extract of Psidium cattleianum biocompatibility by structural and functional cell viability maintenance. Cell viability was analyzed after 1 h, 3 h, 6 h, 10 h and 24 h of incubation by Exclusion Method with trypan blue and MTT assay, using human mononuclear cells (PBMC) and human periodontal ligament (PDL) cells in culture. Positive control used was RPMI cell culture medium and negative control was ethanol 80%. The obtained data were subjected to statistical analysis at a significance level of 5%. By trypan blue assay, ethanolic extract presented a homogeneous performance of PBMC from the 1 h, with average viability between 30 and 50% (p>0.05). By MTT assay, the results showed the efficacy of the ethanolic extract of Psidium cattleianum in maintaining the functional viability of the periodontal ligament cells to 10 h (p>0.05), presenting values greater than the RPMI control. In both methods, it can be observed that the extract properties can neutralize the cytotoxic effect of its diluent ethanol 80%, which showed average viability nearly zero (p <0.05). Thus, biocompatibility by structural and functional cell viability maintenance was considered acceptable to ethanolic extract of Psidium cattleianum and further studies should be performed to indicate its use as an alternative to irrigation solution traditionally used in everyday practice.

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“…For the biopulpectomy treatment, it must help with evacuating organic tissue and draining stagnation. Notwithstanding the kind of treatment to be played out, the inundating arrangement must present biocompatibility, with a specific end goal to keep the respectability of the periapical structures, not advancing aggravation of the rest of the mash tissue and permitting repair [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

MTT Assay of Psidium cattleianum

Martinz¹,

John²,

Hidalgo³

2017

Periodon Prosthodon

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The point of this review was to assess the ethanolic concentrate of Psidium cattleianum biocompatibility by basic and utilitarian cell reasonability support. Cell practicality was broke down after 1, 3, 6, 10 and 24 hours of hatching by Exclusion Method with trypan blue and MTT measure, utilizing human mononuclear cells [PBMC] and human periodontal tendon [PDL] cells in culture. Positive control utilized was RPMI cell culture medium and negative control was ethanol 80%. The got information were subjected to measurable examination at a criticalness level of 5%. By trypan blue examine, ethanolic remove displayed a homogeneous execution of PBMC from the 1 h, with normal reasonability in the vicinity of 30 and half (p>0.05). By MTT examine, the outcomes demonstrated the adequacy of the ethanolic concentrate of Psidium cattleianum in keeping up the utilitarian reasonability of the periodontal tendon cells to 10 h (p>0.05), displaying values more noteworthy than the RPMI control. In both techniques, it can be watched that the concentrate properties can kill the cytotoxic impact of its diluent ethanol 80%, which indicated normal practicality almost zero (p<0.05). The biocompatibility by auxiliary and useful cell feasibility support was viewed as satisfactory to ethanolic concentrate of Psidium cattleianum and further reviews ought to be performed to demonstrate its utilization as another option to water system arrangement generally utilized as a part of regular practice.

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Debris Remaining in the Apical Third of Root Canals after Chemomechanical Preparation by Using Sodium Hypochlorite and Glyde: An In Vivo Study

Cited by 17 publications

References 18 publications

Micro‐CT evaluation of several glide path techniques and ProTaper Next shaping outcomes in maxillary first molar curved canals

Micro‐CT evaluation of several glide path techniques and ProTaper Next shaping outcomes in maxillary first molar curved canals

Ethanolic Extract of Psidium cattleianum as Intracanal Irrigation: An In vitro Study of Cell Viability

MTT Assay of Psidium cattleianum

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