Dean flow assisted cell ordering system for lipid profiling in single-cells using mass spectrometry

Huang, Qin; Mao, Sifeng; Khan, Mashooq; Zhou, Lin; Lin, Jin‐Ming

doi:10.1039/c7cc09608a

Cited by 70 publications

(52 citation statements)

References 33 publications

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“…D) A novel Dean flow‐based microfluidics platform which can be connected to an ESI‐MS to conduct lipid profiling at single‐cell level. Reproduced with permission . Copyright 2018, Royal Society of Chemistry.…”

Section: Single‐cell Omics Analysismentioning

confidence: 99%

“…Huang et al developed a novel Dean flow based microfluidics platform which could be connected to an ESI‐MS to conduct lipid profiling at single‐cell level. This design could reduce the agglomeration and uneven distributions of cells, significantly improving the efficiency of single‐cell mass spectrometry (Figure D) . Zenobi group developed a microarray for mass spectrometry (MAMS) platform which combined hydrophilic reservoirs for massively parallel and automated sample spotting.…”

Section: Single‐cell Omics Analysismentioning

confidence: 99%

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Microfluidic Single‐Cell Omics Analysis

Wang

et al. 2019

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The commonly existing cellular heterogeneity plays a critical role in biological processes such as embryonic development, cell differentiation, and disease progress. Single‐cell omics‐based heterogeneous studies have great significance for identifying different cell populations, discovering new cell types, revealing informative cell features, and uncovering significant interrelationships between cells. Recently, microfluidics has evolved to be a powerful technology for single‐cell omics analysis due to its merits of throughput, sensitivity, and accuracy. Herein, the recent advances of microfluidic single‐cell omics analysis, including different microfluidic platform designs, lysis strategies, and omics analysis techniques, are reviewed. Representative applications of microfluidic single‐cell omics analysis in complex biological studies are then summarized. Finally, a few perspectives on the future challenges and development trends of microfluidic‐assisted single‐cell omics analysis are discussed.

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Section: Single‐cell Omics Analysismentioning

confidence: 99%

Section: Single‐cell Omics Analysismentioning

confidence: 99%

Microfluidic Single‐Cell Omics Analysis

Wang

et al. 2019

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“…For example, the spiral inertial microfluidic channel was integrated with the laser-induced fluorescence (LIF) setup for cell counting at a throughput of 2100 particles/s (Bhagat et al 2010). In addition to the microflow cytometer, Huang et al (2018) realized the lipid profiling in single cells through coupling the spiral inertial microfluidics with the mass spectrometry (see Fig. 3a).…”

Section: Focusing/orderingmentioning

confidence: 99%

Inertial Microfluidics for Single-Cell Manipulation and Analysis

Xiang

Zhang

2019

Handbook of Single Cell Technologies

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Inertial microfluidics has been widely employed as an important sample pretreatment technique for the single-cell analysis because of the distinct advantages of label-free and external field-free operation, high-throughput processing, and simple channel structure. In this chapter, the recent advances on the application of spiral inertial microfluidics as the sample pretreatment tool for single-cell analysis will be systematically summarized. Firstly, the underlying physics of the spiral inertial microfluidics will be introduced for better understanding of the working principle. Secondly, the design guidelines will be provided for the beginners or the researchers from other disciplines to efficiently design the devices of their

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“…This probe consists of a dual-bore quartz tubing that was pulled and fused to a silica capillary for nanoESI-MS analysis [12,13]. The probe showed highly promising results for sampling metabolites and peptides from live single-cells, with over 500 metabolites and peptides detected [17,18]. Traditional capillary microsampling with ESI-IMS-MS was also coupled with fluorescence microscopy to discriminate and select hepatocellular carcinoma cells in specific stages of cell mitosis in order to profile the cellular heterogeneity of dividing cells [39].…”

Section: Addressing the Challenges Of Single-cell Samplingmentioning

confidence: 99%

Recent Advances and New Perspectives in Capillary Electrophoresis-Mass Spectrometry for Single Cell “Omics”

DeLaney

2018

Molecules

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Accurate clinical therapeutics rely on understanding the metabolic responses of individual cells. However, the high level of heterogeneity between cells means that simply sampling from large populations of cells is not necessarily a reliable approximation of an individual cell’s response. As a result, there have been numerous developments in the field of single-cell analysis to address this lack of knowledge. Many of these developments have focused on the coupling of capillary electrophoresis (CE), a separation technique with low sample consumption and high resolving power, and mass spectrometry (MS), a sensitive detection method for interrogating all ions in a sample in a single analysis. In recent years, there have been many notable advancements at each step of the single-cell CE-MS analysis workflow, including sampling, manipulation, separation, and MS analysis. In each of these areas, the combined improvements in analytical instrumentation and achievements of numerous researchers have served to drive the field forward to new frontiers. Consequently, notable biological discoveries have been made possible by the implementation of these methods. Although there is still room in the field for numerous further advances, researchers have effectively minimized various limitations in detection of analytes, and it is expected that there will be many more developments in the near future.

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Dean flow assisted cell ordering system for lipid profiling in single-cells using mass spectrometry

Cited by 70 publications

References 33 publications

Microfluidic Single‐Cell Omics Analysis

Microfluidic Single‐Cell Omics Analysis

Inertial Microfluidics for Single-Cell Manipulation and Analysis

Recent Advances and New Perspectives in Capillary Electrophoresis-Mass Spectrometry for Single Cell “Omics”

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