deadpan, an essential pan-neural gene in Drosophila, encodes a helix-loop-helix protein similar to the hairy gene product.

Bier, Ethan; Vaessin, Harald; Younger-Shepherd, Susan; Jan, L Y; Jan, Y N

doi:10.1101/gad.6.11.2137

Cited by 217 publications

(169 citation statements)

References 102 publications

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“…4D,E; additional data not shown). Whereas all four of these genes are normally expressed in the prospective wing margin (Williams et al 1991;Bier et al 1992;Blochlinger et al 1993; D. There was a notable difference in the ectopic expression of cut, which was induced only in the dorsal compartment of the wing pouch iFig. 4E; Table 2t, whereas vg, bib, and dpn, like wg, were induced in both the dorsal and ventral compartments ( Fig.…”

Section: Ectopic Expression Of D1 Induces Wg Vg and Cut Expressionmentioning

confidence: 99%

Delta is a ventral to dorsal signal complementary to Serrate, another Notch ligand, in Drosophila wing formation.

Feger²,

et al. 1996

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Wing margin formation in Drosophila requires the Notch receptor and, in the dorsal compartment, one of its ligands, Serrate. We provide evidence that Delta, the other known ligand for Notch, is also essential for this process. Delta is required in ventral cells at the dorsal/ventral compartment boundary, where its expression is specifically elevated in second-instar wing discs during wing margin formation. Moreover, ectopic Delta expression induces wingless, vestigial, and cut and causes adult wing tissue outgrowth in the dorsal compartment. This effect is mediated by Notch, because loss of Notch activity suppresses Delta-induced ectopic wing outgrowth. Whereas ectopic expression of Notch or the truncated activated Notch induces cut in both dorsal and ventral compartments, ectopic Delta expression induces cut only in the dorsal compartment and ectopic Serrate induces cut only in the ventral compartment. These observations indicate that Notch-expressing cells in a given compartment have different responses to Delta and Serrate. We propose that Delta and Serrate function as compartment-specific signals in the wing disc, to activate Notch and induce downstream genes required for wing formation.

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Section: Ectopic Expression Of D1 Induces Wg Vg and Cut Expressionmentioning

confidence: 99%

Delta is a ventral to dorsal signal complementary to Serrate, another Notch ligand, in Drosophila wing formation.

Feger²,

et al. 1996

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“…These two genes have similar pan-neural expression patterns but encode unrelated proteins: Dpn contains a bHLH domain, whereas Scrt contains a zinc-finger domain. Loss of function of either of these two pan-neural genes alone does not lead to obvious morphological disruption of the embryonic nervous system [see above for scrt, and Bier et al (1992) for dpn]. Under optimal con-ditions, animals homozygous null for each of these genes occasionally complete development and eclose.…”

Section: Identification O/scrt a New Gene Expressed In All Neuronal mentioning

confidence: 99%

scratch, a pan-neural gene encoding a zinc finger protein related to snail, promotes neuronal development.

Roark

Sturtevant²,

Emery³

et al. 1995

Genes Dev.

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The Drosophila scratch {scrt) gene is expressed in most or all neuronal precursor cells and encodes a predicted zinc finger transcription factor closely related to the product of the mesoderm determination gene snail {sna). Adult flies homozygous for scrt null alleles have a reduced number of photoreceptors in the eye, and embryos lacking the function of both scrt and the pan-neural gene deadpan [dpn], which encodes a basic helix-loop-helix (bHLH) protein, exhibit a significant loss of neurons. Conversely, ectopic expression of a scrt transgene during embryonic and adult development leads to the production of supernumerary neurons. Consistent with scrt functioning as a transcription factor, various genes are more broadly expressed than normal in scrt null mutants. Reciprocally, these same genes are expressed at reduced levels in response to ectopic scrt expression. We propose that scrt promotes neuronal cell fates by suppressing expression of genes promoting non-neuronal cell fates. We discuss the similarities between the roles of the ancestrally related scrt, sna, and escargot [esc] genes in regulating cell fate choices.

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“…We used the following antibodies at the dilutions indicated: rabbit anti-Lk (1:50; gift from D. Nässel, Stockholm University, Stockholm, Sweden); mouse anti--galactosidase (1:2000; Promega); rat anti-Gsb-d (1:3; gift from R. Holmgren, Northwestern University, Evanston, IL, USA); guinea pig anti-Cas (1:500) and guinea pig anti-Hb (1:200) (gifts from T. Isshiki, National Institute of Genetics, Mishima, Japan); rabbit anti-Dpn (1:500; Bier et al, 1992); mouse anti-En (1:50; Developmental Studies Hybridoma Bank #4D9); rabbit anti-Nab (1:1000) and rabbit anti-Pdm1 (1:1000) (Terriente et al, 2007); and rabbit anti-Runt (1:500; gift from A. Brand, University of Cambridge, Cambridge, UK).…”

Section: Immunohistochemistrymentioning

confidence: 99%

A genetic cascade involving klumpfuss, nab and castor specifies the abdominal leucokinergic neurons in the Drosophila CNS

et al. 2010

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SUMMARYIdentification of the genetic mechanisms underlying the specification of large numbers of different neuronal cell fates from limited numbers of progenitor cells is at the forefront of developmental neurobiology. In Drosophila, the identities of the different neuronal progenitor cells, the neuroblasts, are specified by a combination of spatial cues. These cues are integrated with temporal competence transitions within each neuroblast to give rise to a specific repertoire of cell types within each lineage. However, the nature of this integration is poorly understood. To begin addressing this issue, we analyze the specification of a small set of peptidergic cells: the abdominal leucokinergic neurons. We identify the progenitors of these neurons, the temporal window in which they are specified and the influence of the Notch signaling pathway on their specification. We also show that the products of the genes klumpfuss, nab and castor play important roles in their specification via a genetic cascade.

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deadpan, an essential pan-neural gene in Drosophila, encodes a helix-loop-helix protein similar to the hairy gene product.

Cited by 217 publications

References 102 publications

Delta is a ventral to dorsal signal complementary to Serrate, another Notch ligand, in Drosophila wing formation.

Delta is a ventral to dorsal signal complementary to Serrate, another Notch ligand, in Drosophila wing formation.

scratch, a pan-neural gene encoding a zinc finger protein related to snail, promotes neuronal development.

A genetic cascade involving klumpfuss, nab and castor specifies the abdominal leucokinergic neurons in the Drosophila CNS

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