De novo transcriptome sequencing assisted identification of terpene synthases from black pepper (Piper nigrum) berry

George, Johnsy; S, Shelvy; Fayad, Abdulkabeer Muhammed; Umadevi, P.; Angadi, U. B.; Iquebal, Mir Asif; Jaiswal, Sarika; Rai, Anil; Kumar, Dinesh

doi:10.1007/s12298-021-00986-4

Cited by 6 publications

(1 citation statement)

References 20 publications

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“…In this study, in total, 21 terpenoids were detected ( Table 1 ). Although the genes involved in terpenoid biosynthesis are widely studied in different plants [ 20 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ], genes related to the synthesis of terpenoids in sacred lotus have not been studied so far. The precursors of monoterpenes and diterpenes come from the MEP pathway in plastids, while the precursors of sesquiterpenes and triterpenes come from the MVA pathway in the cytoplasm [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analyses Revealed the Key Metabolic Genes and Transcription Factors Involved in Terpenoid Biosynthesis in Sacred Lotus

Qin

Yang

et al. 2022

Molecules

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As the largest group of structurally diverse metabolites, terpenoids are versatile natural compounds that act as metabolism mediators, plant volatiles, and ecological communicators. However, few terpenoid compounds have been identified in plant parts of sacred lotus (Nelumbo nucifera Gaertn.). To elucidate the molecular genetic basis of the terpene biosynthetic pathway, terpenes from different parts of the plant, including seeds (S), young leaves (YL), mature leaves (ML), white flowers (WF), yellow flowers (YF), and red flowers (RF), were identified by LC-MS/MS and the relative contents of the same terpenes in different parts were compared. The results indicate that all plant parts primarily consist of triterpenes, with only minor quantities of sesquiterpenes and diterpenes, and there were differences in the terpene content detected in different plant parts. To illustrate the biosynthesis of various terpenoids, RNA sequencing was performed to profile the transcriptomes of various plant parts, which generated a total of 126.95 GB clean data and assembled into 29,630 unigenes. Among these unigenes, 105 candidate unigenes are involved in the mevalonate (MVA) pathway, methyl-erythritol phosphate (MEP) pathway, terpenoid backbone biosynthesis pathway, and terpenoid synthases pathway. Moreover, the co-expression network between terpene synthase (TPS) and WRKY transcription factors provides new information for the terpene biosynthesis pathway.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analyses Revealed the Key Metabolic Genes and Transcription Factors Involved in Terpenoid Biosynthesis in Sacred Lotus

Qin

Yang

et al. 2022

Molecules

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Examining the genomic features of human and plant-associated Burkholderia strains

Berrios

2022

Arch Microbiol

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Transcriptome sequencing with nanopore technology for acquiring a deeper understanding of abscisic acid regulation of secondary mechanisms in Salvia miltiorrhiza

Kuang

Sun

et al. 2022

Industrial Crops and Products

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De novo transcriptome sequencing assisted identification of terpene synthases from black pepper (Piper nigrum) berry

Cited by 6 publications

References 20 publications

Transcriptome Analyses Revealed the Key Metabolic Genes and Transcription Factors Involved in Terpenoid Biosynthesis in Sacred Lotus

Transcriptome Analyses Revealed the Key Metabolic Genes and Transcription Factors Involved in Terpenoid Biosynthesis in Sacred Lotus

Examining the genomic features of human and plant-associated Burkholderia strains

Transcriptome sequencing with nanopore technology for acquiring a deeper understanding of abscisic acid regulation of secondary mechanisms in Salvia miltiorrhiza

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