De novo transcriptome sequencing and comparative analysis of midgut tissues of four non-model insects pertaining to Hemiptera, Coleoptera, Diptera and Lepidoptera

Gazara, Rajesh Kumar; Cardoso, Christiane; Bellieny-Rabelo, Daniel; Ferreira, Clélia; Terra, Walter R.; Venâncio, Thiago M.

doi:10.1016/j.gene.2017.06.008

Cited by 8 publications

(5 citation statements)

References 51 publications

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“…Alternatively, a subset of these transcripts possibly consists of either orthologues of conserved insect genes, or Schistocerca-specific genes, whose functions have yet to be discovered. Indeed, the abundance of unannotated transcripts is in line with research on other insects (Pauchet et al, 2009;Ma et al, 2012;Gazara et al, 2017;Huang et al, 2017;Dhania et al, 2019;Coutinho-Abreu et al, 2020). Both GO (Figure 1) and COG (Figure 2) functional classifications were consistent with midgut transcriptomic studies in other insect species (Pauchet et al, 2009;Spit et al, 2016b;Huang et al, 2017).…”

Section: Discussionsupporting

confidence: 86%

“…Annotated midgut transcriptomes have become abundantly available, though mostly in holometabolan insect orders, such as Diptera, Lepidoptera and Coleoptera, presumably due to their large species diversity and prevalence of insect pests in these orders ( Sanders et al, 2003 ; Campbell et al, 2005 ; Pauchet et al, 2009 ; Ma et al, 2012 ; Xu et al, 2012 ; Noland et al, 2013 ; Herde and Howe, 2014 ; Spit et al, 2016a ; Zhang et al, 2016 ; Gazara et al, 2017 ; Huang et al, 2017 ; Nadeau et al, 2017 ; Lu et al, 2018 ; Dhania et al, 2019 ; Bonelli et al, 2020 ; Coutinho-Abreu et al, 2020 ; Cui and Franz, 2020 ; Hung et al, 2020 ; Kuang et al, 2021 ; Shu et al, 2021 ; Zou et al, 2021 ; Hixson et al, 2022 ; Jin et al, 2022 ). As expected in the insect midgut, transcripts encoding digestive enzymes, nutrient transporters, and proteins involved in detoxification and peritrophic membrane formation are abundantly present ( Pauchet et al, 2009 ; Ma et al, 2012 ; Gazara et al, 2017 ). The composition of midgut digestive enzymes is often complex, species-dependent and is largely determined by feeding habits, food quality, and specific midgut luminal environments, and presence of symbiotic microbiota ( Holtof et al, 2019 ; Hung et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Post-feeding transcriptomics reveals essential genes expressed in the midgut of the desert locust

Van Lommel,

Holtof,

Tilleman

et al. 2023

Front. Physiol.

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The digestive tract constitutes an important interface between an animal’s internal and external environment. In insects, available gut transcriptome studies are mostly exploratory or look at changes upon infection or upon exposure to xenobiotics, mainly performed in species belonging to holometabolan orders, such as Diptera, Lepidoptera or Coleoptera. By contrast, studies focusing on gene expression changes after food uptake and during digestion are underrepresented. We have therefore compared the gene expression profiles in the midgut of the desert locust, Schistocerca gregaria, between three different time points after feeding, i.e., 24 h (no active digestion), 10 min (the initial stage of feeding), and 2 h (active food digestion). The observed gene expression profiles were consistent with the polyphagous herbivorous lifestyle of this hemimetabolan (orthopteran) species. Our study reveals the upregulation of 576 genes 2 h post-feeding. These are mostly predicted to be associated with digestive physiology, such as genes encoding putative digestive enzymes or nutrient transporters, as well as genes putatively involved in immunity or in xenobiotic metabolism. The 10 min time point represented an intermediate condition, suggesting that the S. gregaria midgut can react rapidly at the transcriptional level to the presence of food. Additionally, our study demonstrated the critical importance of two transcripts that exhibited a significant upregulation 2 h post-feeding: the vacuolar-type H(+)-ATPAase and the sterol transporter Niemann-Pick 1b protein, which upon RNAi-induced knockdown resulted in a marked increase in mortality. Their vital role and accessibility via the midgut lumen may make the encoded proteins promising insecticidal target candidates, considering that the desert locust is infamous for its huge migrating swarms that can devastate the agricultural production in large areas of Northern Africa, the Middle East, and South Asia. In conclusion, the transcriptome datasets presented here will provide a useful and promising resource for studying the midgut physiology of S. gregaria, a socio-economically important pest species.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Post-feeding transcriptomics reveals essential genes expressed in the midgut of the desert locust

Van Lommel,

Holtof,

Tilleman

et al. 2023

Front. Physiol.

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“…Tissue-specific transcriptomes of other lepidopterans have shown that despite the high variability in contig numbers, the number of protein-coding unigenes is consistently between 20,000 and 30,000 [ 9 , 12 , 46 – 48 ]. Insect transcriptomes have been sequenced at higher frequency in the last five years, proving to be useful as a descriptive tool and an invaluable source of sequences for studies researching gene function and design of RNAi experiments [ 2 , 10 , 13 , 49 , 50 ]. In this study, we generated a tissue-specific transcriptome of the sugarcane borer’s gut.…”

Section: Discussionmentioning

confidence: 99%

Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source

et al. 2020

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The sugarcane borer (Diatraea saccharalis, Fabricius, 1794) is a devastating pest that causes millions of dollars of losses each year to sugarcane producers by reducing sugar and ethanol yields. The control of this pest is difficult due to its endophytic behavior and rapid development. Pest management through biotechnological approaches has emerged in recent years as an alternative to currently applied methods. Genetic information about the target pests is often required to perform biotechnology-based management. The genomic and transcriptomic data for D. saccharalis are very limited. Herein, we report a tissue-specific transcriptome of D. saccharalis larvae and a differential expression analysis highlighting the physiological characteristics of this pest in response to two different diets: sugarcane and an artificial diet. Sequencing was performed on the Illumina HiSeq 2000 platform, and a de novo assembly was generated. A total of 27,626 protein-coding unigenes were identified, among which 1,934 sequences were differentially expressed between treatments. Processes such as defence, digestion, detoxification, signaling, and transport were highly represented among the differentially expressed genes (DEGs). Furthermore, seven aminopeptidase genes were identified as candidates to encode receptors of Cry proteins, which are toxins of Bacillus thuringiensis used to control lepidopteran pests. Since plantinsect interactions have produced a considerable number of adaptive responses in hosts and herbivorous insects, the success of phytophagous insects relies on their ability to overcome challenges such as the response to plant defences and the intake of nutrients. In this study, we identified metabolic pathways and specific genes involved in these processes. Thus, our data strongly contribute to the knowledge advancement of insect transcripts, which can be a source of target genes for pest management.

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“…Thus, gene expression of the two fly species was compared at the same physiological stage regardless of age because of the different development times. This approach has been used in comparative transcriptomics in multi-species studies of various insect orders 40 , 41 .…”

Section: Methodsmentioning

confidence: 99%

Differential gene expression in Drosophila melanogaster and D. nigrosparsa infected with the same Wolbachia strain

Detcharoen

Schilling

Arthofer

et al. 2021

Sci Rep

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Wolbachia are maternally inherited endosymbionts that infect nearly half of all arthropod species. Wolbachia manipulate their hosts to maximize their transmission, but they can also provide benefits such as nutrients and resistance against viruses to their hosts. The Wolbachia strain wMel was recently found to increase locomotor activities and possibly trigger cytoplasmic incompatibility in the transinfected fly Drosophilanigrosparsa. Here, we investigated, in females of both D.melanogaster and D.nigrosparsa, the gene expression between animals uninfected and infected with wMel, using RNA sequencing to see if the two Drosophila species respond to the infection in the same or different ways. A total of 2164 orthologous genes were used. The two fly species responded to the infection in different ways. Significant changes shared by the fly species belong to the expression of genes involved in processes such as oxidation–reduction process, iron-ion binding, and voltage-gated potassium-channel activity. We discuss our findings also in the light of how Wolbachia survive within both the native and the novel host.

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De novo transcriptome sequencing and comparative analysis of midgut tissues of four non-model insects pertaining to Hemiptera, Coleoptera, Diptera and Lepidoptera

Cited by 8 publications

References 51 publications

Post-feeding transcriptomics reveals essential genes expressed in the midgut of the desert locust

Post-feeding transcriptomics reveals essential genes expressed in the midgut of the desert locust

Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source

Differential gene expression in Drosophila melanogaster and D. nigrosparsa infected with the same Wolbachia strain

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