De novo transcriptome provides insights into the growth behaviour and resveratrol and trans-stilbenes biosynthesis in Dactylorhiza hatagirea - An endangered alpine terrestrial orchid of western Himalaya

Dhiman, Nisha; Sharma, Nitesh Kumar; Thapa, Pooja; Sharma, Isha; Swarnkar, Mohit Kumar; Chawla, Amit; Shankar, Ravi; Bhattacharya, Amita

doi:10.1038/s41598-019-49446-w

Cited by 21 publications

(14 citation statements)

References 48 publications

(48 reference statements)

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“…Over the years, the market value of crude drugs obtained from the plant has shown an increasing trend which has led to the expansion of its market across different Indian states [13,[47][48][49]. The huge demand in the pharmaceutical sector has driven a flourishing trade of around USD 71,583 [50]. Kala [51] reported that the annual demand for D. hatagirea is approximately 5000 tons, due its use in both traditional as well as modern medicine.…”

Section: Distribution Trade and Consumptionmentioning

confidence: 99%

“…The antioxidant property of compounds obtained from the plant finds its use in the treatment of different human diseases. Indole alkaloids, phenolics (stilbene, e.g., resveratrol), and saponins along with ascorbic acid, phyllo-and naphthloquinones, glucomannan, and carotenoids [50] [80] (Table 3). plant finds its use in the treatment of different human diseases.…”

Section: Bioactive Compounds Of D Hatagireamentioning

confidence: 99%

“…plant finds its use in the treatment of different human diseases. Indole alkaloids, phenolics (stilbene, e.g., resveratrol), and saponins along with ascorbic acid, phyllo-and naphthloquinones, glucomannan, and carotenoids [50] form the active constituents of D. hatagirea. Dactylorhins A-E (glycosidic compounds: dactylorhin A (C40H56O22), dactylorhin B (C40H57O23), dactylorhin C (C14H24O10), dactylorhin D (C27H40O17), dactylorhin E (C27H40O17)) and dactyloses A-B (glycosidic compounds: dactylose A, dactylose B, C12H16O6) obtained from D. hatagirea exhibit a wide range of pharmacological activities [80] (Table 3).…”

Section: Bioactive Compounds Of D Hatagireamentioning

confidence: 99%

“…, dactylorhin E (C27H40O17)) and dactyloses A-B (glycosidic compounds: dactylose A, dactylose B, C12H16O6) obtained from D. hatagirea exhibit a wide range of pharmacological activities [80] (Table 3). 1H-Indole Indole alkaloids [50] Napthalane -1,4-dione Napthoquinone [50] (R)-5-((S)-1,2-dihydroxyethyl)- 7. Pharmacological Importance of D. hatagirea D. hatagirea is a high-value orchid with a broad range of phytochemicals which exert a wide range of beneficial effects.…”

Section: Synonym Structure Referencesmentioning

confidence: 99%

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Dactylorhiza hatagirea (D. Don) Soo: A Critically Endangered Perennial Orchid from the North-West Himalayas

Wani

Kumar

Verma

et al. 2020

Plants

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Dactylorhiza hatagirea (Orchidaceae) is a perennial herb inhabiting sub-alpine to alpine regions, ranging at elevations between 2500 and 5000 m.a.s.l. With palmately lobed rhizome and lanceolate leaves having a sheathing leaf base, it bears pink flowers with purple-colored notches and a curved spur. It finds wide use in ayurveda, siddha, unani, and folk medicine in curing disorders of the circulatory, respiratory, nervous, digestive, skeletal, and reproductive systems, besides boosting the immune system to fight infectious diseases. Secondary metabolites such as dactylorhins A–E, dactyloses A–B, and others exhibit a wide spectrum of pharmacological activities (antioxidant, antimicrobial, antiseptic, anticancer, and immune enhancing activities). Its use as a dietary supplement was found to be beneficial in increasing testosterone levels, resulting in improved sexual desire and arousal. Incessant overexploitation of this medicinally important herb has resulted in the dwindling of its populations in the wild, which has resulted in its classification as a critically endangered plant species. Efforts involving mass reproduction through in vitro (through tissue culture) and in vivo (by vegetative propagation) means are currently being made to maintain the germplasm of this critically endangered orchid. Holding immense significance in clinical research and drug discovery, work on the genomic front (transcriptomics) has recently been carried out to discover the wealth of unexplored genetic information for this perennial herb. The present study is aimed at reviewing different aspects of the orchid to present collective (summarized) information on this medicinally important herb in the present, particularly its botany, ethnobotanical uses, phytochemistry, and pharmacognosy, along with the strategies that need to be adopted to prevent its overexploitation in natural habitats.

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Section: Distribution Trade and Consumptionmentioning

confidence: 99%

Section: Bioactive Compounds Of D Hatagireamentioning

confidence: 99%

Section: Bioactive Compounds Of D Hatagireamentioning

confidence: 99%

Section: Synonym Structure Referencesmentioning

confidence: 99%

See 2 more Smart Citations

Dactylorhiza hatagirea (D. Don) Soo: A Critically Endangered Perennial Orchid from the North-West Himalayas

Wani

Kumar

Verma

et al. 2020

Plants

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“…However, its use in D. hatagirea has not yet been documented. De novo transcriptome analysis of D. hatagirea revealed the presence of differentially expressed genes governing various metabolic pathways and stress tolerance (Dhiman et al, 2019).There exists a vast opportunity in transcriptomic of D. hatagirea, which can be used to reveal various aspects of its uses and values in human life.…”

Section: Genetic Diversitymentioning

confidence: 99%

Dactylorhizahatagirea: A Critical Issue for Research and Development in Nepal

Magar

Dhital

Yamada

et al. 2020

Nepal Journal of Science and Technology

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Dactylorhiza hatagirea (D. don), commonly known as ‘Panchaule’ in Nepal, is a terrestrial orchid found in temperate to the alpine region. It is valued for its ornamental and medicinal use. It is collected haphazardly from nature due to high economic demand. However, its propagation is limited in nature due to its non-endospermous seeds requiring mycorhizal fungal association for germination. This limitation is leading to the extinction of this orchid from nature and has been enlisted as an endangered and threatened species. Its collection and trade are restricted but have been prioritized for research, conservation, and agro-technology development. As very few research has been reported in D. hatagirea, found in Nepal, intensive research, propagation, reintroducing, and commercial cultivation will help control the rhizome collection from nature and meet the economic demand. It will help in the identification and conservation of our local germplasm through its diversity study at the molecular and revenue generation through commercial cultivation under artificial conditions. In this review paper, we discuss the limited research and developments conducted in Dactylorhiza at various levels and ways forward for its research, conservation, and utilization. As the plant is valued for its biochemical constituents, modern biotechnological tools such as transcriptomics and metabolomics can be best utilized to explore the opportunities and increase its production and reintroduction through mass propagation for better commercialization and conservation.

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Illegal harvesting and livestock grazing threaten the endangered orchid Dactylorhiza hatagirea (D. Don) Soó in Nepalese Himalaya

Chapagain

Meilby

Baniya

et al. 2021

Ecology and Evolution

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Harvesting of orchids for medicine and salep production is a traditional practice, and increasing market demand is spurring illegal harvest. Ethno‐ecological studies in combination with the effect of anthropogenic disturbance are lacking for orchids. We compared population density and structure, and tuber biomass of Dactylorhiza hatagirea (D. Don) Soó for three years in two sites: Manang, where harvesting of medicinal plants was locally regulated (protected), and Darchula, where harvesting was locally unregulated (unprotected). Six populations were studied along an elevation gradient by establishing 144 temporary plots (3 × 3 m 2 ) from 3,400 to 4,600 m elevations. Mean density of D. hatagirea was significantly higher in the locally protected (1.31 ± 0.17 plants/m 2 ) than in the unprotected (0.72 ± 0.06 plants/m 2 ) site. The protected site showed stable population density with high reproductive fitness and tuber biomass over the three‐year period. A significant negative effect ( p < .1) of relative radiation index (RRI) on the density of the adult vegetative stage and a positive effect of herb cover on juvenile and adult vegetative stages were found using mixed zero‐inflated Poisson (mixed ZIP) models. The densities of different life stages were highly sensitive to harvesting and livestock grazing. Significant interactions between site and harvesting and grazing indicated particularly strong negative effects of these disturbances on densities of juvenile and adult reproductive stages in the unprotected site. Semi‐structured interviews were conducted with informants ( n = 186) in the villages and at the ecological survey sites. Our interview results showed that at the protected site people are aware of the conservation status and maintain sustainable populations, whereas the opposite was the case at the unprotected site where the populations are threatened. Sustainability of D. hatagirea populations, therefore, largely depends on controlling illegal and premature harvesting and unregulated livestock grazing, thus indicating the need for permanent monitoring of the species.

show abstract

De novo transcriptome provides insights into the growth behaviour and resveratrol and trans-stilbenes biosynthesis in Dactylorhiza hatagirea - An endangered alpine terrestrial orchid of western Himalaya

Cited by 21 publications

References 48 publications

Dactylorhiza hatagirea (D. Don) Soo: A Critically Endangered Perennial Orchid from the North-West Himalayas

Dactylorhiza hatagirea (D. Don) Soo: A Critically Endangered Perennial Orchid from the North-West Himalayas

Dactylorhizahatagirea: A Critical Issue for Research and Development in Nepal

Illegal harvesting and livestock grazing threaten the endangered orchid Dactylorhiza hatagirea (D. Don) Soó in Nepalese Himalaya

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