De Novo transcriptome assembly and differential expression analysis of catharanthus roseus in response to salicylic acid

Abstract

The anti-cancer vinblastine and vincristine alkaloids can only be naturally found in periwinkle (Catharanthus roseus). Both of these alkaloids' accumulations are known to be influenced by salicylic acid (SA). The transcriptome data to reveal the induction effect (s) of SA, however, seem restricted at this time. In this study, the de novo approach of transcriptome assembly was performed on the RNA-Sequencing (RNA-Seq) data in C. roseus. The outcome demonstrated that SA treatment boosted the expression of all the genes in the Terpenoid Indole Alkaloids (TIAs) pathway that produces the vinblastine and vincristine alkaloids. These outcomes supported the time-course measurements of vincristine alkaloid, the end product of the TIAs pathway, and demonstrated that SA spray had a positive impact on transcription and alkaloid synthesis. Additionally, the abundance of transcription factor families including bHLH, C3H, C2H2, MYB, MYB-related, AP2/ ERF, NAC, bZIP, and WRKY suggests a role for a variety of transcription families in response to the SA stimuli. Di-nucleotide and tri-nucleotide SSRs were the most prevalent SSR markers in microsatellite analyses, making up 39% and 34% of all SSR markers, respectively, out of the 77,192 total SSRs discovered.

Comprehensive transcriptomic meta-analysis unveils new responsive genes to methyl jasmonate and ethylene in Catharanthus roseus

“…This gene in the C. roseus plant encodes the enzyme deacetylvindoline-4- O -acetyltransferase, which is involved in the final stages of vindoline biosynthesis. The results obtained in this study were consistent with previous studies wherein the application of various stress treatments led to an increase in DAT expression and consequently enhanced production of important medicinal alkaloids in C. roseus [ 6 , [86] , [87] , [88] , [89] ].…”

“…Terpenoid indole alkaloids (TIA), a prominent subset within the alkaloid family, possess valuable therapeutic properties, particularly in the treatment of diseases like cancer, making them highly relevant on a global scale. TIAs, predominantly found in three plant families “Apocynaceae, Rubiaceae, and Loganiaceae” have been extensively researched [ 6 ]. Within the Apocynaceae family, Catharanthus roseus stands out as a significant medicinal plant, harboring approximately 130 TIAs.…”

Genome-wide identification and expression analysis of TCP family genes in Catharanthus roseus

“…Due to their very low yield, a variety of artificial induction methods have been employed to enrich the C. roseus alkaloids, including callus suspension culture, chemical elicitation and stress inducer ( Zhao et al., 2001 ; Rizvi et al., 2016 ). It is interesting that elicitors enhanced the content of key alkaloids in C. roseus by altering the expression of TIA biosynthesis-related genes ( Soltani et al., 2022 ). Several common elicitors, such as methyl jasmonate (MeJA), salicylic acid (SA), ultraviolet-B (UV-B), and other environmental stresses, have been applied in the accumulation of C. roseus active ingredients ( Ramani and Chelliah, 2007 ; Goklany et al., 2013 ; Soltani et al., 2022 ).…”

“…It is interesting that elicitors enhanced the content of key alkaloids in C. roseus by altering the expression of TIA biosynthesis-related genes ( Soltani et al., 2022 ). Several common elicitors, such as methyl jasmonate (MeJA), salicylic acid (SA), ultraviolet-B (UV-B), and other environmental stresses, have been applied in the accumulation of C. roseus active ingredients ( Ramani and Chelliah, 2007 ; Goklany et al., 2013 ; Soltani et al., 2022 ). However, roles of CrTCP family in the regulation of TIA biosynthesis in C. roseus are largely unknown ( Hao et al., 2021 ).…”