De novo transcriptome assembly and polymorphism detection in two highly divergent evolutionary units of Bosca's newt (Lissotriton boscai) endemic to the Iberian Peninsula

Abstract

This article reports the de novo transcriptome assemblies of two highly divergent evolutionary units of the Iberian endemic Bosca's newt, Lissotriton boscai. These two units are distributed mostly allopatrically but overlap in the central-southwestern coastal region of Portugal. The resources we provide include the raw sequence reads, the assembled transcripts, the annotation and SNPs called for both lineages.

Sma3s: A universal tool for easy functional annotation of proteomes and transcriptomes

“…To overcome the challenges of the current functional annotators we developed Sma3s, which is a standalone tool that has already shown a high accuracy with large sequences datasets, both of proteins and protein‐coding nucleotide sequences . From its first version it has been used in projects with organisms coming from heterogeneous taxonomic divisions such as bacteria , fungus , invertebrates , plants , and animals , and it provided useful results in all cases. Despite being easy to use, Sma3s had some computational requirements that we have now overcome (Supporting Information Section 1.1).…”