De novo transcriptome analysis reveals insights into different mechanisms of growth and immunity in a Chinese soft-shelled turtle hybrid and the parental varieties

Zhang, Haiqi; Xu, Xiuhong; He, Zhongyang; Zheng, Taiying; Shao, Jian-zhong

doi:10.1016/j.gene.2016.12.003

Cited by 20 publications

(21 citation statements)

References 43 publications

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“…These pathways are crucial for organisms to keep normal growth functions (Gao et al, 2013). In other aquatic species, ELD or ELOD genes' functions are also frequently related to growth (Zhang et al, 2017;Wang et al, 2018b). Hence, hybrids' growth heterosis could result from their similar growth-related functions with the more robust progeny, or their transgressive functions beyond both parents.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of mRNA and miRNA Changes in Two Haliotis diversicolor Genotypes and Their Hybrid

et al. 2021

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Heterosis is a widely distributed phenomenon in mollusks. It is vital in aquaculture by bringing beneficial traits into hybrids. People have utilized the heterosis theory in aquaculture for years. However, the molecular basis of heterosis remains elusive. Evident growth and survival heterosis were shown in the hybrid (“Dongyou-1”) of two Haliotis diversicolor geographic genotypes (Japan and Taiwan). To explore the molecular basis underlying the hybrid abalone’s heterosis, we conducted comparative mRNA and miRNA transcriptional analysis in the hybrid and parental genotypes. Differentially expression analysis identified 5,562 differentially expressed genes (DEGs) and 102 differentially expressed miRNAs (DEMs) between the three genotypes. 1,789 DEGs and 71 DEMs were found to be non-additively expressed in the hybrid. Meanwhile, both the expression level dominance pattern (ELD) and expression level overdominance pattern (ELOD) were found in the DEGs and DEMs, showing the existence of dominance and overdominance models in the hybrid’s transcriptome and post-transcriptional regulation. Functional analysis showed the non-additively expressed genes, ELD genes, and ELOD genes were significantly enriched in growth, immunity, and stress response related pathways, while some of the pathways were regulated by the mRNA-miRNA interactions. The expression levels of FGF, C1Q, HC, CAT, SEGPX, and MGST were significantly up-regulated in the hybrid compared to the middle parent value. In conclusion, we identified the existence of non-additivity, dominance, and overdominance models in the transcriptome and miRNAome of the H. diversicolor hybrid; these models facilitate the advantageous parental alleles’ integration into the hybrid, contributing to the hybrid’s growth and survival heterosis.

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Section: Discussionmentioning

confidence: 99%

Integrated Analysis of mRNA and miRNA Changes in Two Haliotis diversicolor Genotypes and Their Hybrid

et al. 2021

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“…Soft‐shelled turtle ( Pelodiscus sinensis ) is a commercially important aquatic species in China, Japan, and Southwest Asia, etc., due to its delicious taste, its high nutritional (high protein and low fat) and medicinal value (decreasing blood pressure, anti‐aging, anti‐fatigue, and helping to enhance immunity) . In China, there is a large market for soft‐shelled turtle, yielding more than 350 000 tons annually . Carapax Trionycis (the shell of the soft‐shelled turtle) is the main by‐product of soft‐shelled turtle industry, which is regarded as waste and typically discarded, resulting in resource wasting and environmental concerns.…”

Section: Introductionmentioning

confidence: 99%

“…In fact, Carapax Trionycis exhibits potential nutraceutical effects and contains various types of bioactive components, such as minerals, collagen proteins, essential amino acids, polypeptides, polysaccharides, and iodine . Moreover, Carapax Trionycis has a long history in Chinese medicine, and has been proven to have effects such as anti‐fibrosis, anti‐tumor, immune regulation, and bone mineral density enhancement . Carapax Trionycis has a considerable calcium content (22%) and has been reported to increase bone density in rats…”

Section: Introductionmentioning

confidence: 99%

“…3,4 In China, there is a large market for soft-shelled turtle, yielding more than 350 000 tons annually. 5 Carapax Trionycis (the shell of the soft-shelled turtle) is the main by-product of soft-shelled turtle industry, which is regarded as waste and typically discarded, resulting in resource wasting and environmental concerns. In fact, Carapax Trionycis exhibits potential nutraceutical effects and contains various types of bioactive components, such as minerals, collagen proteins, essential amino acids, polypeptides, polysaccharides, and iodine.…”

Section: Introductionmentioning

confidence: 99%

“…6 Moreover, Carapax Trionycis has a long history in Chinese medicine, and has been proven to have effects such as anti-fibrosis, antitumor, immune regulation, and bone mineral density enhancement. [5][6][7] Carapax Trionycis has a considerable calcium content (22%) and has been reported to increase bone density in rats. 8 Calcium supplements have attracted increasing attention as adjuvants for the treatment of calcium deficiency and osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

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Bioavailability and prebiotic potential of Carapax Trionycis, a waste from soft‐shelled turtle processing

Wang

Chen

et al. 2020

J Sci Food Agric

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BACKGROUND Carapax Trionycis is the shell of the soft‐shelled turtle. It is rich in minerals, amino acid, peptides, and other nutrients. Current processing and consumption of soft‐shelled turtle leads to the waste of huge quantities of Carapax Trionycis in the form of spent materials. In this study, the bioavailability, prebiotic activity, and physicochemical properties of Carapax Trionycis using different processing methods were investigated. The vinegar‐quenched Carapax Trionycis (V‐CT), fine powders (D0.18, D0.10), and superfine powders (D0.05, D0.025) of Carapax Trionycis were prepared by the vinegar‐quenching method, common grinding, and the superfine grinding method. RESULTS The average particle sizes of D0.18, D0.10, D0.05, and D0.025 were 147.82, 77.35, 36.65, and 2.24 μm, respectively. Superfine grinding changed the surface morphology of Carapax Trionycis and promoted the release of active ingredients. D0.025 had the highest polypeptide (8.15%), polysaccharide (1.21%), total free amino acid (232.36 mg 100 g−1) and water‐soluble extract content (10.74%), and showed the highest calcium release rate (55.64%) after in vitro digestion. The apparent permeability (PAPP) of the resulting Carapax Trionycis samples in the dialysis tubing model and the everted intestinal sac model increased significantly with the decrease in the Carapax Trionycis particle sizes. Furthermore, the five Carapax Trionycis samples significantly stimulated the growth of the tested probiotics and increased lactic acids production after 48 h fermentation compared to the control. The Carapax Trionycis powder prepared by superfine grinding displayed better prebiotic activity than other samples as it significantly induced a greater proliferation of probiotic bacteria and higher production of lactic acid, as well as greater release of free calcium. CONCLUSIONS The results showed that Carapax Trionycis superfine powder D0.025 had the highest active ingredient content, calcium bioavailability, and prebiotic activity. Our approach of developing Carapax Trionycis superfine powder as natural calcium supplement or potential prebiotic would therefore broaden the scope of soft‐shelled turtle processing waste utilization in an eco‐friendly, cost‐effective, and sustainable approach in the future. © 2020 Society of Chemical Industry

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Prevalence and intensity of avian malaria in a quail hybrid zone

Roth

Keiser

Williams

et al. 2021

Ecology and Evolution

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Understanding the proximate and ultimate mechanisms underlying the maintenance of species barriers in localities where closely related congeners overlap is a major focus of evolutionary biology. Recently, parasitism has been put forth as a mechanism that may work to promote the formation, preservation, or breakdown of species barriers (reviewed in Theodosopoulos et al., 2019). Although tests of this hypothesis may be conducted

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De novo transcriptome analysis reveals insights into different mechanisms of growth and immunity in a Chinese soft-shelled turtle hybrid and the parental varieties

Cited by 20 publications

References 43 publications

Integrated Analysis of mRNA and miRNA Changes in Two Haliotis diversicolor Genotypes and Their Hybrid

Integrated Analysis of mRNA and miRNA Changes in Two Haliotis diversicolor Genotypes and Their Hybrid

Bioavailability and prebiotic potential of Carapax Trionycis, a waste from soft‐shelled turtle processing

Prevalence and intensity of avian malaria in a quail hybrid zone

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