De novo transcriptome analysis provides insights into the salt tolerance of Podocarpus macrophyllus under salinity stress

Zou, Lijuan; Li, Taotao; Li, Bingbing; He, Jing; Liao, Chunli; Wang, Lianzhe; Xue, Shouyu; Sun, Tao; Ma, Xuan; Wu, Qiulan

doi:10.1186/s12870-021-03274-1

Cited by 10 publications

(6 citation statements)

References 70 publications

(91 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Cornus hongkongensis subsp. elegans , Vitis amurensis Rupr, and Podocarpus macrophyllus , salt stress led to disorganized cystoid, basidiome, and stroma lamellae structures, which seriously damaged the integrity of the chloroplast ultrastructure [ 17 , 27 , 73 ]. The results of our experiments showed that the grease content increased as the salt concentration increased.…”

Section: Discussionmentioning

confidence: 99%

Salt Stress Inhibits Photosynthesis and Destroys Chloroplast Structure by Downregulating Chloroplast Development–Related Genes in Robinia pseudoacacia Seedlings

Liu

et al. 2023

Plants

View full text Add to dashboard Cite

Soil salinization is an important factor limiting food security and ecological stability. As a commonly used greening tree species, Robinia pseudoacacia often suffers from salt stress that can manifest as leaf yellowing, decreased photosynthesis, disintegrated chloroplasts, growth stagnation, and even death. To elucidate how salt stress decreases photosynthesis and damages photosynthetic structures, we treated R. pseudoacacia seedlings with different concentrations of NaCl (0, 50, 100, 150, and 200 mM) for 2 weeks and then measured their biomass, ion content, organic soluble substance content, reactive oxygen species (ROS) content, antioxidant enzyme activity, photosynthetic parameters, chloroplast ultrastructure, and chloroplast development-related gene expression. NaCl treatment significantly decreased biomass and photosynthetic parameters, but increased ion content, organic soluble substances, and ROS content. High NaCl concentrations (100–200 mM) also led to distorted chloroplasts, scattered and deformed grana lamellae, disintegrated thylakoid structures, irregularly swollen starch granules, and larger, more numerous lipid spheres. Compared to control (0 mM NaCl), the 50 mM NaCl treatment significantly increased antioxidant enzyme activity while upregulating the expression of the ion transport-related genes Na+/H+ exchanger 1(NHX 1) and salt overly sensitive 1 (SOS 1) and the chloroplast development-related genes psaA, psbA, psaB, psbD, psaC, psbC, ndhH, ndhE, rps7, and ropA. Additionally, high concentrations of NaCl (100–200 mM) decreased antioxidant enzyme activity and downregulated the expression of ion transport- and chloroplast development-related genes. These results showed that although R. pseudoacacia can tolerate low concentrations of NaCl, high concentrations (100–200 mM) can damage chloroplast structure and disturb metabolic processes by downregulating gene expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Salt Stress Inhibits Photosynthesis and Destroys Chloroplast Structure by Downregulating Chloroplast Development–Related Genes in Robinia pseudoacacia Seedlings

Liu

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Plant hormone signal transduction is an essential component of plant stress-response signaling pathways, as the most important endogenous substances, phytohormones play critical roles in stress response and growth promotion [ 55 ]. The transcriptome analysis of Podocarpus macrophyllus indicate that the transcription of genes involved in biosynthesis and phytohormone signaling pathways have been altered significantly, and genes related to auxin transport and responsiveness are downregulated in response to salt stress [ 56 ]. In Chenopodium quinoa , salt stress results in a decrease in signaling components of growth-related phytohormones (auxin, BR) and an increase of components in ABA signaling pathway (PP2Cs), while some other important components of ABA pathway (PYLs, SnRK) are downregulated under long-term salt stress [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Promoter activity and transcriptome analyses decipher functions of CgbHLH001 gene (Chenopodium glaucum L.) in response to abiotic stress

Zhou

Wang

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

Background Our previous studies revealed that CgbHLH001 transcription factor (TF) played an important role in abiotic stress tolerance, suggesting that its promoter was a potential target in response to stress signals. In addition, the regulatory mechanism of CgbHLH001 TF is still limited. Results In the present study, a 1512 bp of 5’-flanking sequence of CgbHLH001 gene was identified, and the sequence carried quite a few of cis-acting elements. The gene promoter displayed strong activity and was induced by multiple abiotic stress. A series of 5’-deletions of the promoter sequence resulted in a gradual decrease in its activity, especially, the 5’ untranslated region (UTR) was necessary to drive promoter activity. Further, CgbHLH001 promoter drove its own gene overexpression ectopically at the transcriptional and translational levels, which in turn conferred the stress tolerance to transgenic Arabidopsis. Transcriptome analysis showed that salt stress induced a large number of genes involved in multiple biological regulatory processes. Differentially expressed genes (DEGs) that mediate phytohormone signal transduction and mitogen-activated protein kinase (MAPK) signaling pathway were widely induced and mostly upregulated under salt stress, and the transcription levels in PbHLH::bHLH-overexpressing transgenic lines were higher than that of 35S::bHLH overexpression. Conclusions The CgbHLH001 promoter exhibited a positive response to abiotic stress and its 5’ UTR sequence enhanced the regulation of gene expression to stress. A few important pathways and putative key genes involved in salt tolerance were identified, which can be used to elucidate the mechanism of salt tolerance and decipher the regulatory mechanism of promoters to develop an adaptation strategy for desert halophytes.

show abstract

“…POD (EC1.11.1.7) activity was measured at 470 nm as described in [ 69 ]. CAT (EC1.11.1.6) activity was assayed as described in [ 70 ]. APX (EC1.11.1.11) activity was determined as described by [ 71 ].…”

Section: Methodsmentioning

confidence: 99%

Transcriptome and Low-Affinity Sodium Transport Analysis Reveals Salt Tolerance Variations between Two Poplar Trees

Zhang

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Salinity stress severely hampers plant growth and productivity. How to improve plants’ salt tolerance is an urgent issue. However, the molecular basis of plant resistance to salinity still remains unclear. In this study, we used two poplar species with different salt sensitivities to conduct RNA-sequencing and physiological and pharmacological analyses; the aim is to study the transcriptional profiles and ionic transport characteristics in the roots of the two Populus subjected to salt stress under hydroponic culture conditions. Our results show that numerous genes related to energy metabolism were highly expressed in Populus alba relative to Populus russkii, which activates vigorous metabolic processes and energy reserves for initiating a set of defense responses when suffering from salinity stress. Moreover, we found the capacity of Na+ transportation by the P. alba high-affinity K+ transporter1;2 (HKT1;2) was superior to that of P. russkii under salt stress, which enables P. alba to efficiently recycle xylem-loaded Na+ and to maintain shoot K+/Na+ homeostasis. Furthermore, the genes involved in the synthesis of ethylene and abscisic acid were up-regulated in P. alba but downregulated in P. russkii under salt stress. In P. alba, the gibberellin inactivation and auxin signaling genes with steady high transcriptions, several antioxidant enzymes activities (such as peroxidase [POD], ascorbate peroxidase [APX], and glutathione reductase [GR]), and glycine-betaine content were significantly increased under salt stress. These factors altogether confer P. alba a higher resistance to salinity, achieving a more efficient coordination between growth modulation and defense response. Our research provides significant evidence to improve the salt tolerance of crops or woody plants.

show abstract

De novo transcriptome analysis provides insights into the salt tolerance of Podocarpus macrophyllus under salinity stress

Cited by 10 publications

References 70 publications

Salt Stress Inhibits Photosynthesis and Destroys Chloroplast Structure by Downregulating Chloroplast Development–Related Genes in Robinia pseudoacacia Seedlings

Salt Stress Inhibits Photosynthesis and Destroys Chloroplast Structure by Downregulating Chloroplast Development–Related Genes in Robinia pseudoacacia Seedlings

Promoter activity and transcriptome analyses decipher functions of CgbHLH001 gene (Chenopodium glaucum L.) in response to abiotic stress

Transcriptome and Low-Affinity Sodium Transport Analysis Reveals Salt Tolerance Variations between Two Poplar Trees

Contact Info

Product

Resources

About