De novo transcriptome analysis of the critically endangered alpine Himalayan herb Nardostachys jatamansi reveals the biosynthesis pathway genes of tissue-specific secondary metabolites

Dhiman, Nisha; Kumar, Anil; Kumar, Dinesh; Bhattacharya, Amita

doi:10.1038/s41598-020-74049-1

Cited by 14 publications

(7 citation statements)

References 42 publications

(54 reference statements)

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“…Apart from defense and stress, avonoid and anthocyanins also help in the protection of chlorophyll, as observed in light stress induced leaves of Cornussto lonifera (Field et al 2001). Phenols and terpenoid pathways have also been reported to have been elevated during the successful micropropagation of Nardostachys jatasamani, an important endangered medicinal plant (Dhiman et al 2020). Presence of pathways related to volatile terpenoids, monoterpenoid, limonene and pinene, were seen to be responsible for the scent in Cinnamomum camphora (Hao, et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Upregulated Secondary Metabolite Pathways in Micropropagated Lawsonia Inermis L.

Samanta¹,

Das²,

Ganguli³

et al. 2021

Preprint

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Humans are dependent on plants for medicines, food and raw materials. Among the many plants used, Henna plant (Lawsonia inermis) have been found to be useful, medicinally and commercially. The pigment lawsone (2-hydroxy-1,4-nathaquinone) derived from the plant is the source of Mehendi, used for dying. The plant has a vast repertoire of secondary metabolites, giving the plant medicinal properties like anticarcinogenic, anti-diabetic, anti-inflammatory, immunostimulatory and many more. This creates a huge demand pressure for the plant, which can’t be met by traditional farming. There have been reports of low propagation rate from stem cuttings and seed. So, micropropagation remains the only tool, for the large scale vegetative propagation, without exposing the plant to stress, saving the plant from overexploitation. The plant was established in vitro, with hormonal manipulation. The study shows BAP 4 mg/L and combination hormone of BAP 2 mg/L + Kn 2 mg/L to have best results for shoot bud multiplication. Rooting was seen to be significant in MS medium, highlighting the low cost of maintenance of the plant. Karyotype analysis was performed between in vivo and in vitro plant sets, followed by transcriptome analysis. Karyotype analysis, performed to validate the homogeneity between in vivo and in vitro plants, showed a chromosome number of 30. Transcriptome analysis through the TRAPID platform showed significant difference between in vivo and in vitro plant’s transcript sets. Pathway analysis revealed enriched secondary metabolite pathways of commercial and economic importance in the in vitro generated plantlets as compared to the wild type control.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Upregulated Secondary Metabolite Pathways in Micropropagated Lawsonia Inermis L.

Samanta¹,

Das²,

Ganguli³

et al. 2021

Preprint

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“…The phytochemical levels, particularly the secondary metabolites, showed part-specific or tissue-specific variation of single plants, which corresponds to the fact that gene expression for phytochemical biosynthesis is highly plant-part dependent [24][25][26]. However, some compounds represented significant variations of the contents even in same medicinal part, presumably due to intra-species factors (i.e., different levels of the secondary metabolite biosynthesis, deposit or transformation) or extrinsic factors (i.e., circumstance of growing, harvesting season, degree of sample dryness, etc.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Comparison of the Marker Compounds in Different Medicinal Parts of Morus alba L. Using High-Performance Liquid Chromatography-Diode Array Detector with Chemometric Analysis

Doh

Lee

2020

Molecules

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It is thought that the therapeutic efficacy of Morus alba L. is determined by its biological compounds. We investigated the chemical differences in the medicinal parts of M. alba by analyzing a total of 57 samples (15 root barks, 11 twigs, 12 fruits, and 19 leaves). Twelve marker compounds, including seven flavonoids, two stilbenoids, two phenolic acids, and a coumarin, were quantitatively analyzed using a high-performance liquid chromatography-diode array detector and chemometric analyses (principal component and heatmap analysis). The results demonstrated that the levels and compositions of the marker compounds varied in each medicinal part. The leaves contained higher levels of six compounds, the root barks contained higher levels of four compounds, and the twigs contained higher levels of two compounds. The results of chemometric analysis showed clustering of the samples according to the medicinal part, with the marker compounds strongly associated with each part: mulberroside A, taxifolin, kuwanon G, and morusin for the root barks; 4-hydroxycinnamic acid and oxyresveratrol for the twigs and skimmin; chlorogenic acid, rutin, isoquercitrin, astragalin, and quercitrin for the leaves. Our approach plays a fundamental role in the quality evaluation and further understanding of biological actions of herbal medicines derived from various medicinal plant parts.

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“…First-strand cDNAs were then synthesized using the Verso cDNA Synthesis Kit (Thermo Fisher Scientific, MA, USA) as per the manufacturer’s instruction. For expression analysis, the contigs of PYL9 , ARF18 , ANR1 , SAUR28 , GRF1 , and GID1A were extracted from the online transcriptomic database of N. jatamansi (SRR1241890) and contigs of CAT1 and SOD1 were extracted from the SRR11184224 database [ 24 ] ( Supplementary Figure S1 ). Further using these contig sequences, primers were designed through the primer 3 online software ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…The amplification cycle comprised of 95 °C for 7 min initiation, followed by an amplification step of 40 cycles at 95 °C for 10 s, and 60 °C for 30 s; the melting curve was analyzed with default reaction cycles. The relative quantification method (2−ddCT) was used to quantify the variations using actin as an internal control according to the previously published literature [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

et al. 2022

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Nardostachys jatamansi is a critically endangered medicinal plant and endemic to the Himalayas, having high commercial demand globally. The accumulation of various secondary metabolites in its shoots and roots with antioxidant potential are well-documented in traditional as well as modern medicine systems. In the present study, we first attempted to investigate the impact of citrate (−ve charge, 11.1 ± 1.9 nm) and CTAB (+ve charge, 19.5 ± 3.2 nm) coated gold nanoparticles (AuNPs) on the in vitro proliferation and antioxidant activities of N. jatamansi. Both the nanoparticles differentially affected the morphological and biochemical parameters, chlorophyll content, internal hormone concentration, and antioxidant activities in a concentration-dependent (10–100 µM) manner. Vigorous shooting was observed in half strength MS medium supplemented with IAA (1 mg/L) with 60 µM citrate-AuNPs (46.4 ± 3.7 mm) and 40 µM CTAB-AuNPs (42.2 ± 3.2 mm). Similarly, the maximum number of roots (5.00 ± 0.67 and 5.33 ± 0.58) and root length (29.9 ± 1.5 mm and 27.3 ± 4.8 mm) was reported in half-strength MS medium with IAA (1 mg/L) supplemented with 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs, respectively. In addition, plants growing on MS medium supplemented with 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs showed significantly enhanced photosynthetic pigments (chlorophyll a and b, carotenoids, and total chlorophyll), internal hormone concentration (GA3, IAA, and ABA), and antioxidant activities (total phenolics, flavonoids, DPPH, and SOD enzyme activity). Moreover, the transcript analysis of ANR1, ARF18, PLY9, SAUR28, GID1A, GRF1, SOD, and CAT further confirmed the role of 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs in the improvement in the growth and antioxidant activities of N. jatamansi. Bearing in mind the urgent requirements of the effective conservation measures of this endangered species, the present findings suggest the elicitation of citrate-AuNPs and CTAB-AuNPs would significantly improve the potential applications of N. jatamansi in the medicinal plant-based industry.

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De novo transcriptome analysis of the critically endangered alpine Himalayan herb Nardostachys jatamansi reveals the biosynthesis pathway genes of tissue-specific secondary metabolites

Cited by 14 publications

References 42 publications

Transcriptome Analysis Reveals Upregulated Secondary Metabolite Pathways in Micropropagated Lawsonia Inermis L.

Transcriptome Analysis Reveals Upregulated Secondary Metabolite Pathways in Micropropagated Lawsonia Inermis L.

Quantitative Comparison of the Marker Compounds in Different Medicinal Parts of Morus alba L. Using High-Performance Liquid Chromatography-Diode Array Detector with Chemometric Analysis

Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

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