De novo transcriptome analysis of gonads reveals the sex-associated genes in Chinese hook snout carp Opsariichthys bidens

Tang, Rongkang; Zhu, Yefei; Gan, Wei; Zhang, Yu; Yao, Ziliang; Ren, Jianfeng; Li, Mingyou

doi:10.1016/j.aqrep.2022.101068

Cited by 7 publications

(8 citation statements)

References 62 publications

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“…cDNA was synthesized from 1 µg total RNA (TaKaRa, Kusatsu, Japan) using the M-MLV Reverse Transcription Kit (TaKaRa, Kusatsu, Japan). To obtain cDNA fragments, O. bidens gonad raw sequence data were used [ 18 ]. Primer sequences are shown in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…Its reproductive biology research should be carried out to protect the diversity of O. bidens genetic resources. Recently, we performed an analytical study on the O. bidens gonad transcriptome [ 18 ]. In the present study, we successfully established a long-term-cultured spermatogonia cell line capable of sperm production from the adult testis of O. bidens in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro

Chen

Kan

Zhang

et al. 2022

Biology

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Opsariichthys bidens belongs to the family Cyprinidae and is a small freshwater economic fish widely distributed in China. In recent years, the natural resources of O. bidens have been drastically reduced due to overfishing and the destruction of the water environment. The in vitro culture and long-term preservation of germ stem cells are the key technologies to keep genetic resources from degeneration. However, except for the establishment of the first long-term cultured medaka spermatogonia cell line (SSC) capable of producing sperm in vitro in 2004, no other long-term cultured SSC line has been found in other fish species. In this study, we successfully established another long-term-cultured spermatogonial stem cell line from Opsariichthys bidens (ObSSC). After more than 2 years of culture, ObSSC had a diploid karyotype and stable growth, with the typical gene expression patterns of SSC. Under in vitro culture, ObSSC could be induced to differentiate into sperm and other different types of somatic cells. In vivo, ObSSC could differentiate into different cells of three germ layers upon being transplanted into zebrafish embryos. Our research helps to explore the potential and regulation mechanism of fish SSC differentiation and spermatogenesis in vitro, provides a new way for solving the problem of fish genetic resource degradation and lays a foundation for further research on fish germ cell transplantation.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro

Chen

Kan

Zhang

et al. 2022

Biology

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“…Sc6a9b is a glycine transporter gene whose expression is higher in the ovary and affects ovarian development by affecting neurotransmitters . Using de novo transcriptome analysis, it is revealed that zp4 was differentially expressed in Opsariichthys bidens (Tang et al, 2022). Zar1 plays a role in the early zebrafish ovary by inhibiting ZP gene translation (Miao et al, 2017).…”

Section: Figurementioning

confidence: 99%

Transcriptome sequencing analysis of sex-related genes and miRNAs in the gonads of Mytilus coruscus

Wang

Xia²,

Jawad³

et al. 2022

Front. Mar. Sci.

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Mytilus coruscus is a significant economic species in China’s eastern coastal areas. As a dioecious species, it lacks secondary sexual characteristics, which makes it difficult for selective breeding. However, limited research is carried out on the genetic data regarding reproductive development and gender differentiation. In the current study, de novo transcriptome sequencing analyses were used to detect gonad-specific genes and miRNAs in M. coruscus. By comparing testis and ovary, 159,970 unigenes and 300 miRNAs were obtained totally, of which differentially expressed genes and miRNAs were 9,566 and 25, respectively. Analysis of qRT-PCR showed that cyp26a, dmrt4, foxl2, gdf9, 17β-hsd14, sc6a9, zar1, and zp4 were highly expressed in the ovary as compared to the testis, while sox2 showed lower expression in the ovary. Expression of miR-750-3p, novel 1, and miR-193 was higher in the ovary than that in the testis, whereas the expression of miR-9-5p, miR-9-3p, miR-317, novel 124, miR-2d and miR-263b were lower in the ovary. Furthermore, analysis of miR-317 by Targetscan and MiRanda predicted to target dmrt4 and the luciferase reporter was performed to confirm it. Our research provides a molecular basis for understanding sexual development and reproductive regulation. Further research is needed on the mechanism of gonadal maturation and differentiation in M. coruscus.

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“…Afterwards, these sequences were purified to obtain clean reads by quality control (QC), filtering the reads shorter than 15 nt and longer than 41 nt, the sequences containing N content and sequences with too low Q20 ratio. For primary analysis, the clean sequences were compared with the transcriptome unigene (Tang et al, 2022). Non-coding RNAs were annotated as rRNAs, tRNAs, small nuclear RNAs (snRNAs), and small nucleolar RNAs (snoRNAs).…”

Section: Sequencing Data Analysis and Identification Of Mirnasmentioning

confidence: 99%

“…However, there are few reports on the genes related to reproductive development in O. bidens (Jiang et al, 2021;Lin et al, 2021). Most recently, sex-associated genes were obtained by transcriptome analysis in O. bidens by our lab (Tang et al, 2022). In this research, miRNAs were sequenced from the testis and ovary of O. bidens.…”

Section: Introductionmentioning

confidence: 99%

Identification and expression analysis of sex biased miRNAs in chinese hook snout carp Opsariichthys bidens

Tang

Xu²,

Zhu³

et al. 2022

Front. Genet.

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As an economically important fish, Opsariichthys bidens has obvious sexual dimorphism and strong reproductive capacity, but no epigenetics study can well explain its phenotypic variations. In recent years, many microRNAs involved in the regulation of reproductive development have been explored. In this study, the small RNA libraries of O. bidens on the testis and ovary were constructed and sequenced. A total of 295 known miRNAs were obtained and 100 novel miRNAs were predicted. By comparing testis and ovary libraries, 115 differentially expressed (DE) miRNAs were selected, of which 53 were up-regulated and 62 were down-regulated. A total of 64 GO items (padj < 0.01) and 206 KEGG pathways (padj < 0.01) were enriched in the target gene of miRNA. After that, the expression levels of nine DE miRNAs, including let-7a, miR-146b, miR-18c, miR-202-5p, miR-135c, miR-9-5p, miR-34c-3p, miR-460-5p and miR-338 were verified by qRT-PCR. Furthermore, bidirectional prediction of DE miRNAs and sex-related genes was carried out and the targeting correlation between miR-9-5p and nanos1 was verified by Dual-Luciferase reporter assay. Our findings identified the differentially expressed miRNA and paved the way to new possibilities for the follow-up study on the mechanism of miRNA-mRNA interaction in the gonads of O. bidens.

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De novo transcriptome analysis of gonads reveals the sex-associated genes in Chinese hook snout carp Opsariichthys bidens

Cited by 7 publications

References 62 publications

Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro

Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro

Transcriptome sequencing analysis of sex-related genes and miRNAs in the gonads of Mytilus coruscus

Identification and expression analysis of sex biased miRNAs in chinese hook snout carp Opsariichthys bidens

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