De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus

Abstract

Vibrio parahaemolyticus is a common pathogenic marine bacterium that causes gastrointestinal infections and other health complications, which could be life-threatening to immunocompromised patients. For the past two decades, the pathogenicity of environmental V. parahaemolyticus has increased greatly, and the genomic change behind this phenomenon still needs an in-depth exploration. To investigate the difference in pathogenicity at the genomic level, three strains with different hemolysin expression and biofilm formation capacity were screened out of 69 environmental V. parahaemolyticus strains. Subsequently, 16S rDNA analysis, de novo sequencing, pathogenicity test, and antibiotic resistance assays were performed. Comparative genome-scale interpretation showed that various functional region differences in pathogenicity of the selected V. parahaemolyticus strains were due to dissimilarities in the distribution of key genetic elements and in the secretory system compositions. Furthermore, the genomic analysis-based hypothesis of distinct pathogenic effects was verified by the survival rate of mouse models infected with different V. parahaemolyticus strains. Antibiotic resistance results also presented the multi-directional evolutionary potential in environmental V. parahaemolyticus, in agreement with the phylogenetic analysis results. Our study provides a theoretical basis for better understanding of the increasing pathogenicity of environmental V. parahaemolyticus at the genome level. Further, it has a key referential value for the exploration of pathogenicity and prevention of environmental V. parahaemolyticus in the future.

Identification of Antibacterial Components and Modes in the Methanol-Phase Extract from a Herbal Plant Potentilla kleiniana Wight et Arn

“…Of these, V. parahaemolyticus is a Gram-negative halophilic bacterium that can cause diseases in marine animals, leading to huge economic losses to the aquaculture. V. parahaemolyticus can also cause gastrointestinal infections and other health complications in humans [ 20 ]. B. cereus is a Gram-positive foodborne pathogen that can cause diarrhea and emesis [ 21 ].…”

“…The two common pathogens V. parahemolyticus and S. aureus were chosen for further analysis in this study. The former is the leading sea foodborne pathogen worldwide [ 20 ], while the latter leads to food poisoning, as well as human and animal infections [ 23 ].…”

Biological and transcriptional studies reveal VmeL is involved in motility, biofilm formation and virulence in Vibrio parahaemolyticus

“…The pathogenicity of V. parahaemolyticus is closely related to a variety of virulence factors, which enable it to cause not only gastroenteritis but also wound infections and septicemia ( Daniels et al, 2000 ; Broberg et al, 2011 ). Previous studies have proved that the murine intraperitoneal infection model was successfully established and employed to investigate the pathogenicity of V. parahaemolyticus ( Hiyoshi et al, 2010 ; Matsuda et al, 2019 ; Liu et al, 2021 ). In our study, the virulence, towards HeLa cells and ICR mouse, of V. parahaemolyticus was found to be lessened when in a vmeL mutant compared with wild type.…”

Isolation of the Thermostable β-Glucosidase-Secreting Strain Bacillus altitudinis JYY-02 and Its Application in the Production of Gardenia Blue

“…Isolate JYY-02 was further identified by 16S rRNA gene sequencing. The 16S rRNA gene was amplified from the genomic DNA of isolate JYY-02, extracted according to the method described by Liu et al ( 28 ). Evolutionary relationships were determined using MEGA v7.0.…”