De novo sequencing of Bletilla striata (Orchidaceae) transcriptome and identification of genes involved in polysaccharide biosynthesis

Niu, Junfeng; Zhao, Guangming; Mi, Zeyuan; Chen, Lijun; Liu, Shuai; Wang, Shiqiang; Wang, Donghao; Wang, Zhezhi

doi:10.1590/1678-4685-gmb-2019-0417

Cited by 21 publications

(19 citation statements)

References 47 publications

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“…BSPs are largely composited by the monosaccharides mannose and glucose, thus could be regarded as the glucomannan polymers [ 16 ]. Although the biosynthetic pathway of BSPs have been recently studied by using transcriptome sequencing data [ 10 ], we would like to uncover the detailed regulatory mechanism by integrating metabolic data through an organ-specific way.…”

Section: Resultsmentioning

confidence: 99%

“…2 Organ-specific analysis of the levels of the enzyme-coding genes and the metabolites within the biosynthetic pathway of Bletilla striata polysaccharide (BSP). A The previously reported biosynthetic pathway of BSP [ 10 ] was employed for this analysis. Based on the transcriptome assembly annotated by the KEGG database, nine enzymes could find their coding genes (colored within the pathway) based on the EC numbers.…”

Section: Resultsmentioning

confidence: 99%

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Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)

Tang

et al. 2021

BMC Plant Biol

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Background Bletilla striata is one of the important species belonging to the Bletilla genus of Orchidaceae. Since its extracts have an astringent effect on human tissues, B. striata is widely used for hemostasis and healing. Recently, some other beneficial effects have also been uncovered, such as antioxidation, antiinflammation, antifibrotic, and immunomodulatory activities. As a key step towards a thorough understanding on the medicinal ingredient production in B. striata, deciphering the regulatory codes of the metabolic pathways becomes a major task. Results In this study, three organs (roots, tubers and leaves) of B. striata were analyzed by integrating transcriptome sequencing and untargeted metabolic profiling data. Five different metabolic pathways, involved in polysaccharide, sterol, flavonoid, terpenoid and alkaloid biosynthesis, were investigated respectively. For each pathway, the expression patterns of the enzyme-coding genes and the accumulation levels of the metabolic intermediates were presented in an organ-specific way. Furthermore, the relationships between enzyme activities and the levels of the related metabolites were partially inferred. Within the biosynthetic pathways of polysaccharides and flavonoids, long-range phytochemical transportation was proposed for certain metabolic intermediates and/or the enzymes. Conclusions The data presented by this work could strengthen the molecular basis for further studies on breeding and medicinal uses of B. striata.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)

Tang

et al. 2021

BMC Plant Biol

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“…As the main active ingredient, B. striata polysaccharide (BSP) is widely used in clinical hemostasis, and can also be used as an excellent biopolymer material and pharmaceutical excipient [ 10 – 12 ]. Polysaccharide is rich in the dried pseudobulb with content of 20–50% [ 13 , 14 ]. Moreover, approximately 211 compounds have been extracted from B. striata pseudobulbs, including glucosides, bibenzyls, phenanthrenes, quinones, stilbenes, triterpenoids, phenolics etc.…”

Section: Introductionmentioning

confidence: 99%

Phenotype correlation analysis and excellent germplasm screening of herb Bletilla Rchb. f. based on comprehensive evaluation from thirty-three geographic populations

et al. 2022

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Background The Bletilla genus of Orchidaceae includes plants with great economic value, among which B. striata is the main traditional medicinal plant, and its pseudobulb, known as BaiJi, was first recorded in Shennong’s Classic of Materia Medica. However, there has been little systemic evaluation of the germplasm quality of Bletilla plants in China. In order to comprehensive evaluate the Bletilla resources in China and screen out the candidate phenotypic traits determining yield and/or quality of Bletilla, the variation of phenotypic indicators (pseudobulb, leaf, stem, inflorescence, flower) and active ingredients contents (polysaccharide, total phenolics and militarine) in different populations of B. striata and B. ochracea were investigated through 4 years’ common-garden experiment. Results There were abundant phenotypic variations and significant differences among different populations in the morphological phenotypes, pseudobulb weight and main active ingredient contents. AHBZ, HBLT and HBSN populations showed good prospects for industrial development, presenting higher quality in terms of yield and main active ingredient content. Pseudobulb yield, polysaccharide and total phenol content are positively correlated with phenotypic traits. Militarine content is negatively correlated with almost all indexes. Plant height, leaf width and stem diameter may be important indicators of potential excellent germplasms. Conclusions Bletilla is not strictly geoauthentic medicinal plants. B. ochracea could be accepted as an alternative resource to B. striata. The best harvest period of Bletilla is the third year after cultivation. Plant height, leaf width and stem diameter may be important indicators of potential excellent germplasms. These results provide important information required for the efficient screening and utilization of Bletilla germplasm resources.

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“…Our previous experimental results for B. ochracea polysaccharides (BOP) revealed that they were composed of two simple sugars (glucose and mannose) that had antioxidant and immune system‐enhancing effects (Niu, Wang, et al, 2020; Niu, Zhao, et al, 2020; Wang et al, 2020). However, there exist limited data on the polysaccharides of O. foliosa (OFP) as relates to their structures and bioactivities.…”

Section: Introductionmentioning

confidence: 99%

Screening ofBletilla striata,Bletilla ochraceaandOreorchis foliosadifferential metabolites based on metabolomics

Niu

et al. 2022

Biomedical Chromatography

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As a representative medicinal plant in the Orchidaceae, Bletilla striata plays a variety of pharmacological roles in the clinic. However, the emergence of counterfeit species is affecting the basic medicinal materials source identification process, for which Bletilla ochracea and Oreorchis foliosa of the Orchidaceae are two representative species. For this study, 13 representative B. striata samples, three B. ochracea samples and three O. foliosa samples were selected for the systematic determination of polysaccharide yields and monosaccharide composition, and further detection of secondary metabolites by HPLC–MS. The results revealed that there was a significant difference in the yields of polysaccharides between B. striata and B. ochracea (p = 0.006). Although the polysaccharides of both species were composed of glucose and mannose, the molar ratio of the two monosaccharides was different, suggesting that the structures of the polysaccharides were different. The metabolomics results showed that there were no differences in the types of metabolites between B. striata and B. ochracea; however, there were differences in the contents of these metabolites. Although there was no significant difference in the polysaccharide yields of B. striata and O. foliosa (p = 0.074) and the monosaccharide composition was the same (glucose and mannose), many different metabolites were screened out between them: six compounds such as C36H34O11 existed only in B. striata, while substance C39H54O22 was unique to O. foliosa. Therefore, based on the analysis of the polysaccharide content and monosaccharide composition, combined with phase metabolomics research, a preliminary distinction between B. striata, B. ochracea and O. foliosa was achieved.

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De novo sequencing of Bletilla striata (Orchidaceae) transcriptome and identification of genes involved in polysaccharide biosynthesis

Cited by 21 publications

References 47 publications

Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)

Organ-specific, integrated omics data-based study on the metabolic pathways of the medicinal plant Bletilla striata (Orchidaceae)

Phenotype correlation analysis and excellent germplasm screening of herb Bletilla Rchb. f. based on comprehensive evaluation from thirty-three geographic populations

Screening ofBletilla striata,Bletilla ochraceaandOreorchis foliosadifferential metabolites based on metabolomics

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