De Novo Root Formation in Tobacco Thin Layers is Affected by Inhibition of Polyamine Biosynthesis

Altamura, Maria Maddalena; Torrigiani, Patrizia; Capitani, F.; Scaramagli, Sonia; Bagni, Nello

doi:10.1093/jxb/42.12.1575

Cited by 65 publications

(49 citation statements)

References 13 publications

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“…Accumulation of common PAs has also been correlated with formation of adventitious and lateral roots (Altamura et al 1991), consistent with our results (Fig. 4B).…”

Section: −supporting

confidence: 92%

Changes in the levels of jasmonates and free polyamines induced by Na2SO4 and NaCl in roots and leaves of the halophyte Prosopis strombulifera

et al. 2012

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Prosopis strombulifera, a common legume in high-salinity soils of Argentina, is a useful model for elucidation of salt tolerance mechanisms and specific biochemical pathways in halophytes, since its NaCl tolerance exceeds the limit described for most halophytic plants. We analyzed the effects of the increasing concentration of two main soil salts, Na2SO4 and NaCl, on growth parameters of P. strombulifera, chlorophyll levels, and content of jasmonates (JAs) and polyamines (PAs), which are key molecules involved in stress responses. P. strombulifera showed a halophytic response (growth promotion) to NaCl, but strong growth inhibition by iso-osmotic solutions of Na2SO4. Chlorophyll levels, number of leaves and leaf area were also differentially affected. An important finding was the partial alleviation of SO 2− 4toxicity by treatment with two-salt mixture. JAs are not directly involved in salt tolerance in this species since its levels decrease under all salt treatments. Beneficial effects of Putrescine (Put) accumulation in NaCl treated plants maybe inferred probably associated with the antioxidative defense system. Another novel finding is the accumulation of the uncommon PA cadaverine in roots under high Na2SO4, which may be related to SO 2− 4 toxicity.

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“…Accumulation of common PAs has also been correlated with formation of adventitious and lateral roots (Altamura et al 1991), consistent with our results (Fig. 4B).…”

Section: −supporting

confidence: 92%

Changes in the levels of jasmonates and free polyamines induced by Na2SO4 and NaCl in roots and leaves of the halophyte Prosopis strombulifera

et al. 2012

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“…The characteristic effects of each polyamine on Chamomilla recutita or Origanum majorana could depend on the type of the amine, which is probably due to the variation in polyamine pools within the plant body (Chen and Galston 1985) or the alteration of endogenous polyamine pools as a result of exogenous polyamine application (Altamura et al 1991, Bajaj and Rajam 1995, Ali 2000. The inhibitory effects of polyamines on Origanum majorana roots further substantiate the supposition that the effects of polyamines are tissue, organ and species-specific (Minocha and Minocha 1995).…”

Section: Resultsmentioning

confidence: 60%

The effects of treatment with polyamines on dry matter, oil and flavonoid contents in salinity stressed chamomile and sweet marjoram

Ali¹,

Abbas²,

Kamal³

2007

PLANT SOIL ENVIRON

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The study was undertaken to determine a possible role of polyamines (putrescine, spermidine, spermine) as antioxidants in salt tolerance of Chamomilla recutita and Origanum majorana. Salinity generally induced variable changes in growth, contents of oil and flavonoids of both plants; foliar application of any polyamines counterbalanced the effects of salinity. In general, the degree of stimulation differed according to the type and concentration of the used additive and the type of the plant.

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“…For instance a reduction in the number of root-forming explants has been shown in tobacco thin layers (Altamura et al, 1991), where DL-a-difluoromethylarginine (DFMA) plus DL-a-difluoromethylornithine (DFMO), specific inhibitors of arginine and ornithine decarboxylase respectively, almost totally inhibit rhizogenesis. Cyclohexylamine (CHA), a competitive inhibitor of spermidine synthesis, does not have a strong inhibitory effect on root formation, whereas methylglyoxal-bis(guanyl)hydrazone (MGBG), a SAMDC inhibitor, almost totally depresses the formation of root meristemoids in the explants (Altamura et al, 1991(Altamura et al, , 1993. Morphogenesis in thin layers cultured on the rhizogenic medium is highly disturbed by these inhibitors.…”

Section: Introductionmentioning

confidence: 99%

The plant cell wall is altered by inhibition of polyamine biosynthesis

et al. 1997

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SUMMARYPolyamines (PAs) and the cell wall have important roles in plant morphogenesis. An interaction between cell wall components (pectic substances) and poiyamines is known and inhibition of polyamine biosynthesis induces . inhibition of some morphogenic processes. This is the case of rhizogenesis in tobacco {Nicotiana tabacum L.) thin layers. This paper investigates whether inhibition of polyamine biosynthesis induces modifications in the structure, shape and size of the primary cell walls in tobacco thin layers cultured on a rhizogenic medium, and whether polyamines (putrescine and spermidine) administered in combination with their corresponding inhibitors DL-a-difluoromethylornithine + DL-a-difluoromethyiarginine (DFMO + DFMA), cyclohexylamine (CHA), methylglyoxai-bis(guanylhydrazone) (MGBG) restore cell wall characteristics. In treatments with polyamine inhibitors, cell hypertrophy occurred in the lowermost layer of the explant in contact wdth the medium. Many unusual features were observed in the walls of the hypertrophic cells: an amorphous structure, loosening of the fibrillar component; detachment between contiguous cells, lysis of wall components and an uneven thickness often giving a wavy appearance (in transverse walls). The inhibitors reduced cell wall thickness, and caused a weak positive PATAg reaction for polysaccharides. Polyamines restored cell wall thickness and, in general, the other wall features. Our results show that polyamines are necessary for maintaining cell w^all characteristics by strengthening the links between cell-wall components.

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De Novo Root Formation in Tobacco Thin Layers is Affected by Inhibition of Polyamine Biosynthesis

Cited by 65 publications

References 13 publications

Changes in the levels of jasmonates and free polyamines induced by Na2SO4 and NaCl in roots and leaves of the halophyte Prosopis strombulifera

Changes in the levels of jasmonates and free polyamines induced by Na2SO4 and NaCl in roots and leaves of the halophyte Prosopis strombulifera

The effects of treatment with polyamines on dry matter, oil and flavonoid contents in salinity stressed chamomile and sweet marjoram

The plant cell wall is altered by inhibition of polyamine biosynthesis

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