De novo post-pollen mitosis II tobacco pollen tube transcriptome

Hafidh, Said; Breznenová, Katarína; Honys, David

doi:10.4161/psb.20745

Cited by 17 publications

(21 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Analysis of different indicators confirmed that the transcriptome database generated is of high quality and provides an encompassing coverage of the tobacco male gametophyte transcriptome. A comparison of our transcriptome database with the draft tobacco genome indicated that consistent with previous estimations based on microarray data [ 21 , 22 ] roughly 30% of all tobacco genes are expressed in the male gametophyte.…”

Section: Introductionsupporting

confidence: 83%

“…Although a high-quality tobacco male gametophyte transcriptome database has been missing to date, valuable information concerning global gene expression at different stages of tobacco male gametophyte development has been obtained based on microarray analyses using the Agilent 44 K tobacco gene chip. These analyses indicated that 13,966 genes are expressed in mature pollen [ 21 ], 14,100 genes in pollen tubes grown for 4 h [ 21 ] and 14,420 genes in pollen tubes grown for 24 h [ 22 ]. As the number of genes in the tobacco draft genomes was recently estimated to be somewhere between 81,000 and 94,000 [ 20 ], the 44 K gene chip can obviously only represent roughly 50% of all tobacco genes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptome profiling of tobacco (Nicotiana tabacum) pollen and pollen tubes

et al. 2017

View full text Add to dashboard Cite

BackgroundPollen tube growth is essential for plant reproduction and represents a widely employed model to investigate polarized cell expansion, a process important for plant morphogenesis and development. Cellular and regulatory mechanisms underlying pollen tube elongation are under intense investigation, which stands to greatly benefit from a comprehensive understanding of global gene expression profiles in pollen and pollen tubes. Here, RNA sequencing technology was applied to de novo assemble a Nicotiana tabacum male gametophytic transcriptome and to compare transcriptome profiles at two different stages of gametophyte development: mature pollen grains (MPG) and pollen tubes grown for six hours in vitro (PT6).ResultsDe novo assembly of data obtained by 454 sequencing of a normalized cDNA library representing tobacco pollen and pollen tube mRNA (pooled mRNA isolated from mature pollen grains [MPG] and from pollen tubes grown in vitro for 3 [PT3] or 6 [PT6] hours) resulted in the identification of 78,364 unigenes. Among these unigenes, which mapped to 24,933 entries in the Sol Genomics Network (SGN) N. tabacum unigene database, 24,672 were predicted to represent full length cDNAs. In addition, quantitative analyses of data obtained by Illumina sequencing of two separate non-normalized MPG and PT6 cDNA libraries showed that 8979 unigenes were differentially expressed (differentially expressed unigenes: DEGs) between these two developmental stages at a FDR q-value of <0.0001. Interestingly, whereas most of these DEGs were downregulated in PT6, the minor fraction of DEGs upregulated in PT6 was enriched for GO (gene ontology) functions in pollen tube growth or fertilization.ConclusionsA major output of our study is the development of two different high-quality databases representing the tobacco male gametophytic transcriptome and containing encompassing information about global changes in gene expression after pollen germination. Quantitative analyses of these databases 1) indicated that roughly 30% of all tobacco genes are expressed in the male gametophyte, and 2) support previous observations suggesting a global reduction of transcription after pollen germination. Interestingly, a small number of genes, many of which predicted to function in pollen tube growth or fertilization, were found to be upregulated in elongating pollen tubes despite globally reduced transcription.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3972-3) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of tobacco (Nicotiana tabacum) pollen and pollen tubes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Such a fact was already demonstrated for Arabidopsis mitochondrial phosphoproteome where phosphopeptide enrichment led to the identification of novel phosphorylation sites that were not previously identified by the alternative approaches (38). As mentioned above, active transcription in activated pollen grain as long as 24 h of pollen tube growth has been shown (54,55). Here, we identified several transcription factors, most of which contained a zinc finger motif.…”

Section: Discussionmentioning

confidence: 69%

“…Tobacco pollen activation and subsequent pollen tube growth was originally shown to be vitally dependent on translation but almost independent of transcription (53). Although our recent microarray transcriptomic analyses revealed a number of mRNAs being synthesized during pollen tube growth even after 24 h of cultivation (54,55), many of the transcripts in the desiccated mature pollen are stored in EDTA/puromycine-resistant particles (EPPs). These particles contain parts of ribosomes and translation apparatus together with mRNAs (56,57) and the translation of EPP-stored mRNAs starts after pollen activation.…”

Section: Discussionmentioning

confidence: 99%

Phosphoproteomics Profiling of Tobacco Mature Pollen and Pollen Activated in vitro

Fíla

Radau

Matros

et al. 2016

Molecular & Cellular Proteomics

Self Cite

View full text Add to dashboard Cite

Tobacco mature pollen has extremely desiccated cytoplasm, and is metabolically quiescent. Upon re-hydration it becomes metabolically active and that results in later emergence of rapidly growing pollen tube. These changes in cytoplasm hydration and metabolic activity are accompanied by protein phosphorylation. In this study, we subjected mature pollen, 5-min-activated pollen, and 30-min-activated pollen to TCA/acetone protein extraction, trypsin digestion and phosphopeptide enrichment by titanium dioxide. The enriched fraction was subjected to nLC-MS/MS. We identified 471 phosphopeptides that carried 432 phosphorylation sites, position of which was exactly matched by mass spectrometry. These 471 phosphopeptides were assigned to 301 phosphoproteins, because some proteins carried more phosphorylation sites. Of the 13 functional groups, the majority of proteins were put into these categories: transcription, protein synthesis, protein destination and storage, and signal transduction. Many proteins were of unknown function, reflecting the fact that male gametophyte contains many specific proteins that have not been fully functionally annotated. The quantitative data highlighted the dynamics of protein phosphorylation during pollen activation; the identified phosphopeptides were divided into seven groups based on the regulatory trends. The major group comprised mature pollen-specific phosphopeptides that were dephosphorylated during pollen activation. Several phosphopeptides representing the same phosphoprotein had different regulation, which pinpointed the complexity of protein phosphorylation and its clear functional context. Collectively, we showed the first phosphoproteomics data on activated pollen where the position of phosphorylation sites was clearly demonstrated and regulatory kinetics was resolved. Molecular & Cellular

show abstract

“…расположение их в конечных зонах фигуры будущего деления; нарушения в развитии микроспоры, приводящие к отсутствию дифференцирующего митоза, связаны в том числе и с изменением полюсов деления. Эти мнения подтверждены экспериментальными данными, свидетельствующими, что поляризация протопласта клетки предшествует дифференцирующему митозу и является отдельным от митоза процессом (Hafidh et al, 2012).…”

Section: перемещения ядер и клеток в развивающемся пыльцевом зерне злunclassified

Nuclei and Cells Movements in Developed Pollen Grains During Organogenesis of Cereal Anther as Integrated System

Zinatullina¹

2019

ÈKOBIOTEH

View full text Add to dashboard Cite

В обзорной статье приведены результаты анализа данных, посвященных органогенезу пыльника злаков как сложной интегрированной системы. Особое внимание уделено цитологическому феномену перемещения ядер и клеток на определенных фазах микроспорогенеза и микрогаметогенеза. Анализируются внутренние и внешние факторы, обусловливающие такие закономерные перемещения в интегрированной системе пыльника. Подчёркивается, что системный подход к органогенезу пыльника важен в исследованиях не только in vivo, но и в биотехнологических исследованиях in vitro.

show abstract

De novo post-pollen mitosis II tobacco pollen tube transcriptome

Cited by 17 publications

References 9 publications

Transcriptome profiling of tobacco (Nicotiana tabacum) pollen and pollen tubes

Transcriptome profiling of tobacco (Nicotiana tabacum) pollen and pollen tubes

Phosphoproteomics Profiling of Tobacco Mature Pollen and Pollen Activated in vitro

Nuclei and Cells Movements in Developed Pollen Grains During Organogenesis of Cereal Anther as Integrated System

Contact Info

Product

Resources

About