De novo expression of intercellular-adhesion molecule 1 in melanoma correlates with increased risk of metastasis.

Johnson, Judith P.; Stade, Barbara; Holzmann, Bernhard; Schwäble, W.; Riethmüller, Gert

doi:10.1073/pnas.86.2.641

Cited by 301 publications

(172 citation statements)

References 23 publications

Supporting

Mentioning

159

Contrasting

Unclassified

Order By: Relevance

“…Relation between biological behavior of human melanoma cell lines in nude mice and expression of MAAs. [26,33], Mainly based on the marked higher expression of ICAM-1 in melanoma metastases than in primary melanomas it was suggested that ICAM-1 could have a functional role in the process of dissemination [16,25]. However, in our animal model system we did not find an induction of ICAM-1 expression in metastatic lesions indicating that in this system there seems no important role for this adhesion molecule in metastasis.…”

Section: Discussionmentioning

confidence: 69%

Antigen expression of metastasizing and non-metastasizing human melanoma cells xenografted into nude mice

Muijen¹,

Cornelissen²,

Jansen³

et al. 1991

Clin Exp Metast

110

100

View full text Add to dashboard Cite

In order to study differences in antigen expression related to the different stages of the process of metastasis of human melanoma cell lines, we determined the expression pattern of a series of well-characterized genes in a set of human melanoma cell lines with different metastatic behavior in nude mice. This set included non-metastatic (IF6, 530), sporadically metastatic (M14, Mel 57), and frequently metastatic (BLM, MV3) cell lines after subcutaneous inoculation, To study the phenotype of these cell lines both the cultured cells and representative samples of local tumors at the inoculation site and their metastases in the lungs were immunostained with a panel of monoclonal antibodies directed against melanocytic differentiation or progres sion antigens. Although most cell lines (IF6, 530, M14 and Mel 57) showed HLA-DR expression in vitro, these antigens were lacking in all xenografted lesions studied with exception of the 530 cell line. 530 Xenografts, however, showed a dramatic down-regulation of HLA-DR compared with the cell line in vitro. The same phenomenon was seen with respect to ICAM-1 expression.The expression of all other antigens studied in xenografts, both in subcutan eous tumors and in lung lesions, was in general comparable to that in the melanoma cell lines in vitro, with exception of the 530 cell line. In all melanoma cell lines except 530 the degree of intra-and interlesional hetero geneity regarding the expression of all antigens studied was limited. Remark ably, comparison of the immunophenotype of the frequently metastasizing (BLM, MV3) and the sporadically (M14, Mel 57) or non-metastasizing (IF6, 530) cell lines showed that the two frequently metastasizing cell lines had marked expression of the progression antigens VLA-2 and epidermal growth factor receptor, and lack of expression of the differentiation antigen NKIbeteb. These findings warrant further studies on the role of these antigens in the process of metastasis of human melanoma cells in nude mice. IntroductionA number of monoclonal antibodies (MAbs) have been developed that recognize antigens expressed by human melanocytic cells. These melanomaassociated antigens (MAAs) include differentiation antigens, progression anti gens and other antigens, such as functional antigens. Differentiation antigens are

show abstract

Section: Discussionmentioning

confidence: 69%

Antigen expression of metastasizing and non-metastasizing human melanoma cells xenografted into nude mice

Muijen¹,

Cornelissen²,

Jansen³

et al. 1991

Clin Exp Metast

110

100

View full text Add to dashboard Cite

show abstract

“…11 Studies have suggested a correlation between the expression of CD54 and the progression of skin and uveal melanomas. 12,13 Expression of CD54 was detected in 81-85% of human uveal melanoma specimens. 13,14 Reduced expression of CD54 in primary uveal melanomas has been associated with increased metastatic disease.…”

mentioning

confidence: 99%

Effect of an anti‐CD54 (ICAM‐1) monoclonal antibody (UV3) on the growth of human uveal melanoma cells transplanted heterotopically and orthotopically in SCID mice

Wang

Coleman

Pop

et al. 2005

Intl Journal of Cancer

View full text Add to dashboard Cite

We have shown that administration of a novel anti-CD54 monoclonal antibody (UV3) results in long-term survival of SCID mice bearing human myeloma xenografts. Previous studies have demonstrated a link between the expression of CD54 and the progression of uveal melanoma. Our study assessed the expression of CD54 on 7 human uveal melanoma cell lines and 3 cell lines established from uveal melanoma metastases. In vivo studies examined the efficacy of systemic and local administration of UV3 antibody on the progression of uveal melanoma cells transplanted either heterotopically or orthotopically into SCID mice. Five of the 7 primary uveal melanoma cell lines and all 3 of the metastases cell lines expressed CD54. Intraperitoneal injection of either IgG or F(ab 0 ) 2 fragments of UV3 significantly inhibited the growth of subcutaneous and intraocular melanomas. Subconjunctival injection of either IgG or F(ab 0 ) 2 fragments of UV3 produced a significant reduction in the growth of intraocular melanomas, even if the antibody was administered after the appearance of intraocular tumors. The results indicate that both primary and metastatic human uveal melanoma cells express CD54. The marked inhibition of intraocular and subcutaneous uveal melanoma progression suggests that UV3 antibody is a promising therapeutic agent for further evaluation in patients with uveal melanoma. This is especially noteworthy, as no existing therapeutic modality prevents metastasis of uveal melanoma or prolongs the survival of patients with uveal melanoma. ' 2005 Wiley-Liss, Inc.Key words: anterior chamber; eye; CD54; immunotherapy; uveal melanoma Uveal melanoma is the most common intraocular malignancy in adults and occurs with a frequency of 6 to 7 cases per one million adults. 1 Although cutaneous and uveal melanomas share a neural crest origin, they differ significantly in their epidemiological, 2 cytogenetic 3 , metastatic 4-6 and immunological 4 characteristics. One of the remarkable differences between cutaneous and uveal melanomas is their metastatic behavior. Although skin melanomas metastasize to almost any organ, uveal melanoma displays a propensity to spread to the liver. Indeed, liver metastases are present in up to 95% of the patients who die from uveal melanomas. [7][8][9][10] Although there have been advances in the treatment of primary uveal melanomas, the 5-year survival rate for uveal melanoma patients has not improved in the past 30 years. 1,10 Moreover, no therapeutic modality prevents the metastasis of uveal melanoma or prolongs the survival of patients. 11 Studies have suggested a correlation between the expression of CD54 and the progression of skin and uveal melanomas. 12,13 Expression of CD54 was detected in 81-85% of human uveal melanoma specimens. 13,14 Reduced expression of CD54 in primary uveal melanomas has been associated with increased metastatic disease. 13 CD54 is an important ligand for leukocytes and is believed to be involved in immune surveillance. 15,16 Intravenous administration of MAb against human CD54 (U...

show abstract

“…Other studies have indicated changes in expression of several cell surface glycoproteins in metastasis. Johnson et al (1989) demonstrated that the expression of intracellularadhesion molecule 1, in human melanoma correlates with increased risk of metastases. Tandon et al (1990) described association between the 323/A3 surface glycoprotein and poor prognosis in breast cancer.…”

Section: Resultsmentioning

confidence: 99%

The expression of 5T4 antigen in colorectal and gastric carcinoma

Starzyńska

Rahi²,

Stern³

1992

Br J Cancer

View full text Add to dashboard Cite

Summary The expression of 5T4, an oncotrophoblast cell surface antigen was examined in 72 colorectal and 27 gastric carcinomas, with immunoperoxidase technique, on frozen sections.Highly significant association was found between 5T4 expression in the malignant cells and metastatic spread. The results suggest that the appearance of 5T4 molecules in cancer cells reflects a change which may contribute to the development of metastatic potential.It has been shown that certain human tumour tissues and cell lines express membrane antigens otherwise found only on placental trophoblast and absent on normal tissues (e.g. Loke et al., 1980;McLaughlin et al., 1982). These proteins may exist to protect the semi-allogeneic foetus from immunological rejection and in a similar way allow tumour cells to evade host immunity. An improvement of understanding the role of these antigens in human cancer is likely to be the basis for achieving new approaches to both its identification and treatment. The 5T4 trophoblast cell surface antigen (Hole & Stern, 1988; shows a restricted pattern of expression in normal human tissues, but the antigen is present in a wide variety of transformed cell lines (Hole & Stern, 1988) and carcinomas (Southall et al., 1990;Jones et al., 1990). In these investigations of 5T4 antigen expression in limited numbers of different human carcinomas, no conclusions could be drawn as to possible correlations with established prognostic factors.The objective of the present study was to determine the relationship between 5T4 expression, tumour growth and stage of disease, in order to investigate the difference, if any, between 5T4 positive and negative carcinomas from the colon and stomach. Materials and methodsSeventy-two colorectal and 27 gastric carcinomas were included in this study. Gastric specimens and 33 colorectal cancers were obtained at the Pomeranian Medical Academy, Szczecin, Poland, the other colorectal neoplasms were from various Departments of Surgery, Manchester. The histological type and stage of tumour (Table II) as well as the grading (Table III) were assessed from routine examination of paraffin-embedded sections, stained with haematoxylin and eosin. The stage of grouping was made according to the criteria of Dukes and to the criteria of the Japanese Research Society, for colorectal and gastric cancer respectively. ResultsThe results of immunohistochemical evaluation are summarised in Table I. Overall the proportion of tumour specimens labelled with mAb 5T4 was 85% for colorectal and 81% for gastric carcinoma. However, there were two distinct patterns of immunohistochemical staining, either detection of positive labelling of the malignant cells and surrounding stroma (40% of colorectal and 56% of the gastric tumours) or strong positive reactivity of stromal elements alone (45% of colorectal and 26% of gastric tumours). When the tumour cells were labelled for 5T4 antigen, there was reactivity in all or nearly all malignant cells as evidenced by congruency with cytokeratin labelling. Only 11 colore...

show abstract

De novo expression of intercellular-adhesion molecule 1 in melanoma correlates with increased risk of metastasis.

Cited by 301 publications

References 23 publications

Antigen expression of metastasizing and non-metastasizing human melanoma cells xenografted into nude mice

Antigen expression of metastasizing and non-metastasizing human melanoma cells xenografted into nude mice

Effect of an anti‐CD54 (ICAM‐1) monoclonal antibody (UV3) on the growth of human uveal melanoma cells transplanted heterotopically and orthotopically in SCID mice

The expression of 5T4 antigen in colorectal and gastric carcinoma

Contact Info

Product

Resources

About