De novo design of a pH-triggered self-assembled β-hairpin nanopeptide with the dual biological functions for antibacterial and entrapment

Li, Qiuke; Li, Jinze; Yu, Weikang; Wang, Zhihua; Li, Jiawei; Feng, Xingjun; Wang, Jiajun; Shan, Anshan

doi:10.1186/s12951-021-00927-z

Cited by 39 publications

(29 citation statements)

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“…The MIC values of P19 in the presence of physiological salt concentrations increased somewhat were a little raised, especially in the presence of Na + . This is primarily arising due to the high ionic strength, which can interfere with the electrostatic attraction between cationic peptides and anionic bacterial lipid bilayers [31][32][33]. And this is a common phenomenon for cationic peptides.…”

Section: Discussionmentioning

confidence: 99%

Selective Antifungal Activity and Fungal Biofilm Inhibition of Tryptophan Center Symmetrical Short Peptide

Chou

et al. 2021

IJMS

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Candida albicans, an opportunistic fungus, causes dental caries and contributes to mucosal bacterial dysbiosis leading to a second infection. Furthermore, C. albicans forms biofilms that are resistant to medicinal treatment. To make matters worse, antifungal resistance has spread (albeit slowly) in this species. Thus, it has been imperative to develop novel, antifungal drug compounds. Herein, a peptide was engineered with the sequence of RRFSFWFSFRR-NH2; this was named P19. This novel peptide has been observed to exert disruptive effects on fungal cell membrane physiology. Our results showed that P19 displayed high binding affinity to lipopolysaccharides (LPS), lipoteichoic acids (LTA) and the plasma membrane phosphatidylinositol (PI), phosphatidylserine (PS), cardiolipin, and phosphatidylglycerol (PG), further indicating that the molecular mechanism of P19 was not associated with the receptor recognition, but rather related to competitive interaction with the plasma membrane. In addition, compared with fluconazole and amphotericin B, P19 has been shown to have a lower potential for resistance selection than established antifungal agents.

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Section: Discussionmentioning

confidence: 99%

Selective Antifungal Activity and Fungal Biofilm Inhibition of Tryptophan Center Symmetrical Short Peptide

Chou

et al. 2021

IJMS

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“…It has been long reported that the net positive charge of peptides was of vital importance in the antimicrobial performance ( Jiang et al, 2008 ). Furthermore, it was confirmed that the morphological change of bacteria to the peptides could cause the disruption of the bacterial membrane and leakage of the cytoplasmic content ( Li et al, 2021 ; Wang et al, 2021 ).…”

Section: Discussionmentioning

confidence: 91%

The Trp-rich Antimicrobial Amphiphiles With Intramolecular Aromatic Interactions for the Treatment of Bacterial Infection

Wang

et al. 2021

Front. Microbiol.

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Antibiotic resistance is emerging as a hot issue with the abuse and overuse of antibiotics, and the shortage of effective antimicrobial agents against multidrug resistant bacteria creates a huge problem to treat the threatening nosocomial skin and soft tissue infection. Antimicrobial peptides (AMPs) exhibite enormous potential as one of the most promising candidates of antibiotic to fight against pathogenic infections because of its unique membrane penetration mechanism to kill pathogens, whereas the clinical application of AMPs still faces the challenges of production cost, stability, safety, and design strategy. Herein, a series of Trp-rich peptides was designed following the principle of paired Trp plated at the ith and ith+4 position on the backbone of peptides, based on the template (VKKX)4, where X represents W, A, or L, to study the effect of intramolecular aromatic interactions on the bioactivity of AMPs. Through comparing the antimicrobial performance, hemolysis, cytotoxicity, and stability, VW5 which is equipped with the characters of direct antimicrobial efficacy (GM=1.68μM) and physical destruction of bacterial membrane (SEM and electron microscopy) stood out from the engineering peptides. VW5 also performed well in mice models, which could significantly decrease the bacterial colony (VW5 vs infection group, 12.72±2.26 vs 5.52±2.01×109CFU/abscess), the area of dermo-necrosis (VW5 vs infection group, 0.74±0.29 vs 1.86±0.98mm2) and the inflammation cytokine levels at the abscess site without causing toxicity to the skin. Overall, this study provides a strategy and template to diminish the randomness in the exploration and design of novel peptides.

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“…Meanwhile, it was identified that diphenylalanine is the most minimalistic nature motif of the peptide-based antimicrobial building block to this day. The pioneering work of Schnaider et al (2017) demonstrated that diphenylalanine formed unbranched nanotubes while inhibiting the growth of E. coli at 125 µg/ml, triggering the upregulation of stress-response FIGURE 10 | The phenomenon of bacterial entrapment caused by AMP-SAP in vitro (Li et al, 2021). Copyright 2021 by the BioMed Central.…”

Section: Diphenylalanine (Ff)mentioning

confidence: 99%

“…Examples are represented by DNA double-helix or biological membranes ( Mouritsen, 1998 ; Douglas et al, 2009 ) and proteins that fold into secondary and tertiary structures ( Lentz et al, 2000 ). Meanwhile, recent studies on innate immunity have also found that some peptides could form ordered nanostructures to interact with bacterial membranes or entrap bacteria to form sediment ( Figure 10 ) ( Yeaman and Yount, 2003 ; Chairatana and Nolan, 2017 ; Li et al, 2021 ). Wehkamp J. et al ( Chu et al, 2012 ; Schroeder et al, 2015 ; Chairatana et al, 2016 ) reported that human α-defensin 6 (HD6), a 32-aa cysteine-rich peptide, is stored in Paneth cells (specialized secretory epithelial cells) granules as an 81-residue pro-peptide (proHD6) ( Bevins, 2013 ).…”

Section: Self-assembled Antimicrobial Peptidesmentioning

confidence: 99%

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Nanostructured Antimicrobial Peptides: Crucial Steps of Overcoming the Bottleneck for Clinics

Yang

et al. 2021

Front. Microbiol.

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The security issue of human health is faced with dispiriting threats from multidrug-resistant bacteria infections induced by the abuse and misuse of antibiotics. Over decades, the antimicrobial peptides (AMPs) hold great promise as a viable alternative to treatment with antibiotics due to their peculiar antimicrobial mechanisms of action, broad-spectrum antimicrobial activity, lower drug residue, and ease of synthesis and modification. However, they universally express a series of disadvantages that hinder their potential application in the biomedical field (e.g., low bioavailability, poor protease resistance, and high cytotoxicity) and extremely waste the abundant resources of AMP database discovered over the decades. For all these reasons, the nanostructured antimicrobial peptides (Ns-AMPs), based on a variety of nanosystem modification, have made up for the deficiencies and pushed the development of novel AMP-based antimicrobial therapies. In this review, we provide an overview of the advantages of Ns-AMPs in improving therapeutic efficacy and biological stability, reducing side effects, and gaining the effect of organic targeting and drug controlled release. Then the different material categories of Ns-AMPs are described, including inorganic material nanosystems containing AMPs, organic material nanosystems containing AMPs, and self-assembled AMPs. Additionally, this review focuses on the Ns-AMPs for the effect of biological activities, with emphasis on antimicrobial activity, biosecurity, and biological stability. The “state-of-the-art” antimicrobial modes of Ns-AMPs, including controlled release of AMPs under a specific environment or intrinsic antimicrobial properties of Ns-AMPs, are also explicated. Finally, the perspectives and conclusions of the current research in this field are also summarized.

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De novo design of a pH-triggered self-assembled β-hairpin nanopeptide with the dual biological functions for antibacterial and entrapment

Cited by 39 publications

References 50 publications

Selective Antifungal Activity and Fungal Biofilm Inhibition of Tryptophan Center Symmetrical Short Peptide

Selective Antifungal Activity and Fungal Biofilm Inhibition of Tryptophan Center Symmetrical Short Peptide

The Trp-rich Antimicrobial Amphiphiles With Intramolecular Aromatic Interactions for the Treatment of Bacterial Infection

Nanostructured Antimicrobial Peptides: Crucial Steps of Overcoming the Bottleneck for Clinics

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