De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males

Žáček, Petr; Prchalová-Horňáková, Darina; Tykva, R.; Kindl, Jiří; Vogel, Heiko; Svatoš, Aleš; Pichová, Iva

doi:10.1002/cbic.201200684

Cited by 30 publications

(38 citation statements)

References 38 publications

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“…Cephalic labial glands are well known as the synthesis site of sex (marking) pheromones in bumblebee males (Žáček et al, 2013). Their role in females, where they are drastically reduced, remains elusive.…”

Section: Resultsmentioning

confidence: 99%

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Jedlička

Ernst

Votavová³

et al. 2016

Front. Physiol.

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Understanding the social evolution leading to insect eusociality requires, among other, a detailed insight into endocrine regulatory mechanisms that have been co-opted from solitary ancestors to play new roles in the complex life histories of eusocial species. Bumblebees represent well-suited models of a relatively primitive social organization standing on the mid-way to highly advanced eusociality and their queens undergo both, a solitary and a social phase, separated by winter diapause. In the present paper, we characterize the gene expression levels of major endocrine regulatory pathways across tissues, sexes, and life-stages of the buff-tailed bumblebee, Bombus terrestris, with special emphasis on critical stages of the queen's transition from solitary to social life. We focused on fundamental genes of three pathways: (1) Forkhead box protein O and insulin/insulin-like signaling, (2) Juvenile hormone (JH) signaling, and (3) Adipokinetic hormone signaling. Virgin queens were distinguished by higher expression of forkhead box protein O and downregulated insulin-like peptides and JH signaling, indicated by low expression of methyl farnesoate epoxidase (MFE) and transcription factor Krüppel homolog 1 (Kr-h1). Diapausing queens showed the expected downregulation of JH signaling in terms of low MFE and vitellogenin (Vg) expressions, but an unexpectedly high expression of Kr-h1. By contrast, reproducing queens revealed an upregulation of MFE and Vg together with insulin signaling. Surprisingly, the insulin growth factor 1 (IGF-1) turned out to be a queen-specific hormone. Workers exhibited an expression pattern of MFE and Vg similar to that of reproducing queens. Males were characterized by high Kr-h1 expression and low Vg level. The tissue comparison unveiled an unexpected resemblance between the fat body and hypopharyngeal glands across all investigated genes, sexes, and life stages.

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Section: Resultsmentioning

confidence: 99%

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Jedlička

Ernst

Votavová³

et al. 2016

Front. Physiol.

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“…CLGS are species‐specific secretions synthesized de novo by cephalic labial glands (Žáček et al . ). CLGS consist of a complex mixture of (mainly aliphatic) compounds, with several main components (Coppée et al .…”

Section: Methodsmentioning

confidence: 97%

Methods for species delimitation in bumblebees (Hymenoptera, Apidae, Bombus): towards an integrative approach

et al. 2015

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Delimitation of closely related species is often hindered by the lack of discrete diagnostic morphological characters. This is exemplified in bumblebees (genus Bombus). There have been many attempts to clarify bumblebee taxonomy by using alternative features to discrete morphological characters such as wing shape, DNA, or eco‐chemical traits. Nevertheless each approach has its own limitations. Recent studies have used a multisource approach to gather different lines of speciation evidence in order to draw a strongly supported taxonomic hypothesis in bumblebees. Yet, the resulting taxonomic status is not independent of selected evidence and of consensus methodology (i.e. unanimous procedure, majority, different weighting of evidence). In this article, we compare taxonomic conclusions for a group of taxonomically doubtful species (the Bombus lapidarius‐group) obtained from the four commonly used lines of evidence for species delimitation in bumblebees (geometric morphometric of wing shape, genetic differentiation assessment, sequence‐based species delimitation methods and differentiation of cephalic labial gland secretions). We ultimately aim to assess the usefulness of these lines of evidence as components of an integrative decision framework to delimit bumblebee species. Our results show that analyses based on wing shape do not delineate any obvious cluster. In contrast, nuclear/mitochondrial, sequence‐based species delimitation methods, and analyses based on cephalic labial gland secretions are congruent with each other. This allows setting up an integrative decision framework to establish strongly supported species and subspecies status within bumblebees.

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“…A commonly used as chemical markers for resolving species status (Bertsch & Schweer, ; Rasmont et al, ), the male cephalic labial gland secretions (CLGS) were analysed. The CLGS are species‐specific secretions synthesized de novo by cephalic labial glands (Žáček et al, ). They consist of a complex mixture of mainly aliphatic compounds (Coppée, Terzo, Valterová, & Rasmont, ; Lecocq et al, ), with several main components (Lecocq, Dellicour, et al, ).…”

Section: Methodsmentioning

confidence: 99%

Too strict or too loose? Integrative taxonomic assessment of Bombus lapidarius complex (Hymenoptera: Apidae)

et al. 2019

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The latest progress of the taxonomy is the use of integrative approach for species delimitation based on a multisource dataset. However, the taxonomic decision that should be made when convergence between the different lines of evidence is not observed remains debated. Here, we investigate the consequences of the application of an ‘integration by cumulation’ approach on the taxonomic statuses within the Bombus lapidarius complex when using an integrative taxonomic framework (i.e. nuclear and mitochondrial markers along with reproductive traits) compared with a strict ‘integration by congruence’ method. Our results show similar taxonomic conclusions whatever the decision‐making approach used except for one taxon. According to the differentiation observed in our integrative taxonomic framework, recent divergence time and other field observations for this taxon, we assume that a too strict decision‐making method could fail to detect recently diverged species. This is exemplified by the new species Bombus bisiculus sp. n. occurring in South Italy and Sicily.

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De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males

Cited by 30 publications

References 38 publications

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Methods for species delimitation in bumblebees (Hymenoptera, Apidae, Bombus): towards an integrative approach

Too strict or too loose? Integrative taxonomic assessment of Bombus lapidarius complex (Hymenoptera: Apidae)

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