De novo assembly of a fruit transcriptome set identifies AmMYB10 as a key regulator of anthocyanin biosynthesis in Aronia melanocarpa

Abstract

Aronia is a group of deciduous fruiting shrubs, of the Rosaceae family, native to eastern North America. Interest in Aronia has increased because of the high levels of dietary antioxidants in Aronia fruits. Using Illumina RNA-seq transcriptome analysis, this study investigates the molecular mechanisms of polyphenol biosynthesis during Aronia fruit development. Six A. melanocarpa (diploid) accessions were collected at four fruit developmental stages. De novo assembly was performed with 341 million clean reads from 24 samples and assembled into 90,008 transcripts with an average length of 801 bp. The transcriptome had 96.1% complete according to Benchmarking Universal Single-Copy Orthologs (BUSCOs). The differentially expressed genes (DEGs) were identified in flavonoid biosynthetic and metabolic processes, pigment biosynthesis, carbohydrate metabolic processes, and polysaccharide metabolic processes based on significant Gene Ontology (GO) biological terms. The expression of ten anthocyanin biosynthetic genes showed significant up-regulation during fruit development according to the transcriptomic data, which was further confirmed using qRT-PCR expression analyses. Additionally, transcription factor genes were identified among the DEGs. Using a transient expression assay, we confirmed that AmMYB10 induces anthocyanin biosynthesis. The de novo transcriptome data provides a valuable resource for the understanding the molecular mechanisms of fruit anthocyanin biosynthesis in Aronia and species of the Rosaceae family.

Transcriptomic analysis of tuberous root in two sweet potato varieties reveals the important genes and regulatory pathways in tuberous root development

“…A large number of studies have shown that MYB, bHLH, and WD40 transcription factors were the regulators of flavonoid biosynthesis, and the results also showed that IbMYB1 controls the biosynthesis of anthocyanins in sweet potato [ 71 ]. It was found that 10 anthocyanin biosynthesis genes were significantly up-regulated during Aronia melanocarpa fruit development [ 72 ]. Hence, it shows that anthocyanin biosynthesis related-genes may be involved in the tuberous root development in sweet potato, and their regulatory mechanism should be studied in the next step.…”

Investigating the induction of polyphenol biosynthesis in the cultured Cycolocarya paliurus cells and the stimulatory mechanism of co-induction with 5-aminolevulinic acid and salicylic acid

“…The high-quality fragment reads (quality value ≥20) were spliced by software Trinity (software version number: 2.4.0) ( Jonathan et al, 2022 ). All the assembled unigenes were searched against public protein databases, such as NCBI non-redundant protein sequences (NR) and Swiss-Prot for homology comparison.…”

“…All the assembled unigenes were searched against public protein databases, such as NCBI non-redundant protein sequences (NR) and Swiss-Prot for homology comparison. Only those matches with E-value below 10–5 were deemed significant ( Park et al, 2021 ; Jonathan et al, 2022 ). Furthermore, gene function prediction and classification were assigned based on the database of the Gene Ontology (GO) and Eukaryotic Orthologous Groups of proteins (KOG).…”

15-cis-Phytoene Desaturase and 15-cis-Phytoene Synthase Can Catalyze the Synthesis of β-Carotene and Influence the Color of Apricot Pulp

“…Recent studies have shown that kiwifruit [2,3], kumquat [4], tomato [5][6][7], apple [8], carrot [9], banana [10], and apricot [11] contain major accumulations of carotenoids. Furthermore, anthocyanins mainly accumulate in mulberry [12], Chinese Jujube [13], Chokecherry [14], peach [15], citrus [16], grape [17], persimmon [18], black chokeberry [19], eggplant [20], and onion [21].…”