“…Analysis of metabolites by thin-layer chromatography revealed that these primary metabolites were incorporated into withanolide A, demonstrating that withanolide A is de novo synthesized within roots from primary isoprenogenic precursors (Sangwan et al, 2008). Recently, EST and transcriptome databases generated from the root tissue of W. somnifera also revealed presence of biosynthethic genes in roots (Gupta, Goel, et al, 2013;Senthil, Wasnik, Kim, & Yang, 2010). Expression analysis of some of the key biosynthetic genes in root tissue further showed their active role in biosynthesis of withanolides Bhat et al, 2012;Gupta, Agarwal, et al, 2013;Gupta, Sharma, Kumar, Vishwakarma, & Khan, 2012).…”