De novo assembly, characterization and comparative transcriptome analysis of gonads reveals sex-biased genes in Coreoperca whiteheadi

De Novo Assembly, Characterization, and Comparative Transcriptome Analysis of Mature Male and Female Gonads of Rabbitfish (Siganus oramin) (Bloch & Schneider, 1801)

“…It seems that bmp1 also participates in the process of ovary maturation in rabbitfish. Similar to results in S. hollandi [17] and C. whiteheadi [18], we also found that gdf3 was highly expressed in the ovaries of the rabbitfish. However, gdf3 has been demonstrated to be required for mesendoderm formation and dorsal-ventral patterning, and mutation of gdf3 causes abnormal development of zebrafish embryos [36].…”

“…In addition, researchers also found that knocking out amh or amhrII in female fish caused ovarian termination [32]. Similar to results in Coreoperca whiteheadi [18] and S. hollandi [17], zp3 and zp4 also displayed ovary-biased expression in rabbitfish, indicating that they might also be essential for ovary development as a consequence of mediating the combination of sperm and ovum [17].…”

“…According to previous similar studies [15,17,18], a total of six mature S. oramin (about one year old) were obtained before spawning (three males and three females) from the waters of Daya Bay, Guangdong Province, in China in May 2023. The temperature of the environment ranged from 27 to 33 • C, with humidity of about 70-80%.…”

“…Recently, next-generation sequencing technology has rapidly developed, and transcriptome sequencing technology has been increasingly applied in studies related to fish sex and reproduction. Based on transcriptome sequencing technology, many reproduction-related genes have been uncovered in a large number of fish species, such as yellow catfish (Pleteobagrus fulvidraco) [10], spotted knifejaw (Oplegnathus punctatus) [11], Chinese tongue sole (Cynoglossus semilaevis) [12], silver sillago (Sillago sihama) [13], spot-fin porcupine fish (Diodon hystrix) [14], spotted scat (Scatophagus argus) [15], mandarin fish (Siniperca chuatsi) [16], army fish (Spinibarbus hollandi) [17], white head mandarin fish (Coreoperca whiteheadi) [18], and so on. Based on these reproduction-related genes, researchers can explore their expression and function more pertinently.…”

A delayed and unsynchronized ovary development as revealed by transcriptome of brain and pituitary of Coilia nasus

“…RNA-seq technology has been widely used to profile genes involved in gonad development and their molecular mechanisms in fish ( Du et al, 2017 ). Gender-specific transcriptomes have revealed a number of genes involved in gonad development, including medaka ( Oryzias latipes ) ( Dechaud et al, 2021 ), threespine ( Gasterosteus aculeatus ) ( Kaitetzidou et al, 2022 ), Amur sturgeon ( Acipenser schrenckii ) ( Zhang et al, 2016 ), red-tail catfish ( Hemibagrus wyckioides ) ( Wei et al, 2023 ) and Coreoperca whiteheadi ( Liu et al, 2023 ). Transcript profiles were generated from sexually mature and immature gonads of fish species to identify sex-associated molecular markers ( Amberg et al, 2013 ) and sex-related genes ( Cao et al, 2022 ).…”