De novo assembly and characterization of the transcriptome in the desiccation-tolerant moss Syntrichia caninervis

Gao, Bei; Zhang, Daoyuan; Li, Xiaoshuang; Hong-yuan, Yang; Wood, Andrew J.

doi:10.1186/1756-0500-7-490

Cited by 58 publications

(59 citation statements)

References 89 publications

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“…This moss has evolved a constitutive protection system to alleviate the damage caused by dehydration (Oliver et al 2000, 2009). Syntrichia caninervis and Bryum argenteum are both classified as DT mosses, and their responses to desiccation have been widely studied (Gao et al 2014, 2015; Li et al 2014; Wu et al 2012; Zheng et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Dehydration-responsive features of Atrichum undulatum

Xiao

Bao

et al. 2016

J Plant Res

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Drought is an increasingly important limitation on plant productivity worldwide. Understanding the mechanisms of drought tolerance in plants can lead to new strategies for developing drought-tolerant crops. Many moss species are able to survive desiccation—a more severe state of dehydration than drought. Research into the mechanisms and evolution of desiccation tolerance in basal land plants is of particular significance to both biology and agriculture. In this study, we conducted morphological, cytological, and physiological analyses of gametophytes of the highly desiccation-tolerant bryophyte Atrichum undulatum (Hedw.) P. Beauv during dehydration and rehydration. Our results suggested that the mechanisms underlying the dehydration–recovery cycle in A. undulatum gametophytes include maintenance of membrane stability, cellular structure protection, prevention of reactive oxygen species (ROS) generation, elimination of ROS, protection against ROS-induced damage, and repair of ROS-induced damage. Our data also indicate that this dehydration–recovery cycle consists not only of the physical removal and addition of water, but also involves a highly organized series of cytological, physiological, and biochemical changes. These attributes are similar to those reported for other drought- and desiccation-tolerant plant species. Our findings provide major insights into the mechanisms of dehydration-tolerance in the moss A. undulatum.

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Section: Introductionmentioning

confidence: 99%

Dehydration-responsive features of Atrichum undulatum

Xiao

Bao

et al. 2016

J Plant Res

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“…We identified 26 Unigenes as ScELIPs in the reference transcriptome [10], 6of which have complete open reading frames (ORFs): Unigenes 13021, 40120, 40121, 68225, 16576, and 8044. Unigene 16576 and 8044 each contain 94 predicted amino acids that were of short length to known ELIPs (Table S1).…”

Section: Multiple Sequence Alignment and Phylogenetic Analysismentioning

confidence: 99%

“…Publicly available ELIP sequences of A. thaliana and Physcomitrella patens were used to screen the transcriptome of S. caninervis [10] for single copy transcripts (designated as unigenes) that were putative ELIP coding sequences. We translated each unigene to obtain the amino acid sequence using ORF finder.…”

Section: Bioinformatic Analysis Of Syntrichia Caninervis Elip Sequencesmentioning

confidence: 99%

“…Syntrichia caninervis and Bryum argenteum, as predominant moss species of the Gobi Desert [7], rapidly reassemble thylakoid proteins during rehydration after a desiccation event, presumably as a means to recover photosynthesis and growth [8]. These mosses also quickly increase transcript abundance for genes involved in the light reactions, photorespiration, and other photosynthesis-related genes [9,10], which presumably speeds the recovery of photosynthesis and carbon assimilation [11]. In Syntrichia caninervis, the increase in transcript abundance for genes involved in photosynthesis accompanies the rapid restoration and reorganization of PSII and increased chlorophyll synthesis [12].To maintain normal function under high-light stress conditions plants induce light-stress proteins, the Early Light-Inducible Proteins (ELIPs), as part of the protective response [13].…”

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Functional Aspects of Early Light-Induced Protein (ELIP) Genes from the Desiccation-Tolerant Moss Syntrichia caninervis

Liu

Zhang

Hong-yuan

et al. 2020

IJMS

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The early light-induced proteins (ELIPs) are postulated to act as transient pigment-binding proteins that protect the chloroplast from photodamage caused by excessive light energy. Desert mosses such as Syntrichia caninervis, that are desiccation-tolerant and homoiochlorophyllous, are often exposed to high-light conditions when both hydrated and dry ELIP transcripts are accumulated in response to dehydration. To gain further insights into ELIP gene function in the moss S. caninervis, two ELIP cDNAs cloned from S. caninervis, ScELIP1 and ScELIP2 and both sequences were used as the basis of a transcript abundance assessment in plants exposed to high-light, UV-A, UV-B, red-light, and blue-light. ScELIPs were expressed separately in an Arabidopsis ELIP mutant Atelip. Transcript abundance for ScELIPs in gametophytes respond to each of the light treatments, in similar but not in identical ways. Ectopic expression of either ScELIPs protected PSII against photoinhibition and stabilized leaf chlorophyll content and thus partially complementing the loss of AtELIP2. Ectopic expression of ScELIPs also complements the germination phenotype of the mutant and improves protection of the photosynthetic apparatus of transgenic Arabidopsis from high-light stress. Our study extends knowledge of bryophyte photoprotection and provides further insight into the molecular mechanisms related to the function of ELIPs. must thus find a balance between the requirements for efficient light harvesting and the danger of accumulating light-induced damage when light absorption exceeds the photosynthesis capacity.Bryophytes, as one of the earliest diverging lineages of the extant land plants, are believed to have faced tremendous challenges in occupying a variety of ecological niches many of which were characterized by water deficits, high-light intensities, and increased exposure to UV radiation. In meeting these challenges bryophytes have evolved an efficient photosynthetic apparatus and photoprotection mechanisms. The drought-tolerant mosses, Rhytidium rugosum and Ceratodon purpureus, have demonstrably improved photosystem II (PSII) chlorophyll fluorescence quenching when dehydrated that is hypothesized to provide a means to dissipate excess excitation energy as heat [5]. The desert moss, Pterygoneurum lamellatum, exhibits damage to the photosynthetic apparatus that directly correlates to the rate of desiccation; F v /F m values measured for gametophores dried slowly were similar to undried controls but were significantly reduced in plants dried quickly [6]. Syntrichia caninervis and Bryum argenteum, as predominant moss species of the Gobi Desert [7], rapidly reassemble thylakoid proteins during rehydration after a desiccation event, presumably as a means to recover photosynthesis and growth [8]. These mosses also quickly increase transcript abundance for genes involved in the light reactions, photorespiration, and other photosynthesis-related genes [9,10], which presumably speeds the recovery of photosynthesis and carbon assimilation [11]. In ...

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“…In the OneKP 71 pilot study, transcriptome data for a total of 92 plants were generated and among them 11 moss species were included 72 (Wickett et al, 2014). The transcriptomes in two desert mosses Syntrichia caninervis and Bryum argenteum were reported in 73 our previous studies (Gao et al, 2014;Gao et al, 2017) and a recent phylotranscriptomic study in pleurocarpous mosses 74 (Hypnales) generated a large set of transcriptomic data . 75…”

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confidence: 95%

Integrated phylogenomic analyses reveal recurrent ancestral large-scale duplication events in mosses

Gao

Chen

et al. 2019

Preprint

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20• Mosses (Bryophyta) are a key group occupying important phylogenetic position for understanding land plant 21(embryophyte) evolution. The class Bryopsida represents the most diversified lineage and contains more than 95% of the 22 modern mosses, whereas the other classes are by nature species-poor. The phylogeny of mosses remains elusive at present. 23 24• Recurrent whole genome duplications have shaped the evolution trajectory of angiosperms, but little is known about the 25 genome evolutionary history in mosses. It remains to be answered if there existed a historical genome duplication event 26 associated with the species radiation of class Bryopsida. 27 28• Here, the high-confidence moss phylogeny was generated covering major moss lineages. Two episodes of ancient genomic 29 duplication events were elucidated by phylogenomic analyses, one in the ancestry of all mosses and another before the 30 separation of the Bryopsida, Polytrichopsida and Tetraphidopsida, with estimated ages of the gene duplications clustered 31 around 329 and 182 million year ago, respectively. 32 33• The third episode of polyploidy event (termed ψ ) was tightly associated with the early diversification of Bryopsida with an 34 estimated age of ~87 million years. By scrutinizing the phylogenetic timing of duplicated syntelogs in Physcomitrella 35 patens, the WGD1 and WGD2 events were confidently re-recognized as the ψ event and the Funarioideae duplication event 36 (~65 mya), respectively. Together, our findings unveiled four episodes of polyploidy events in the evolutionary past of 37Physcomitrella patens. 38 39

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De novo assembly and characterization of the transcriptome in the desiccation-tolerant moss Syntrichia caninervis

Cited by 58 publications

References 89 publications

Dehydration-responsive features of Atrichum undulatum

Dehydration-responsive features of Atrichum undulatum

Functional Aspects of Early Light-Induced Protein (ELIP) Genes from the Desiccation-Tolerant Moss Syntrichia caninervis

Integrated phylogenomic analyses reveal recurrent ancestral large-scale duplication events in mosses

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