De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer

Abstract

Analysis of the pore formation mechanisms of transmembrane peptides and proteins potentially provides an insight into cellular biology because of their significant roles in biological systems. Here, we describe the procedures to analyze the pore formation mechanisms of transmembrane peptides and proteins by measuring de-insertion currents of biological nanopores that can be obtained by unzipping the lipid bilayers.