DDX6 Helicase Behavior and Protein Partners in Human Adipose Tissue-Derived Stem Cells during Early Adipogenesis and Osteogenesis

Abstract

DDX6 helicase is an RNA-binding protein involved in different aspects of gene expression regulation. The roles played by DDX6 depend on the complexes associated with it. Here, for the first time, we characterize the protein complexes associated with DDX6 in human adipose tissue-derived stem cells (hASCs) and analyze the dynamics of this helicase under different conditions of translational activity and differentiation. The results obtained demonstrated that the DDX6 helicase is associated with proteins involved in the control of mRNA localization, translation and metabolism in hASCs. DDX6 complexes may also assemble into more complex structures, such as RNA-dependent granules, the abundance and composition of which change upon inhibited translational activity. This finding supports the supposition that DDX6 is possibly involved in the regulation of the mRNA life cycle in hASCs. Although there was no significant variation in the protein composition of these complexes during early adipogenic or osteogenic induction, there was a change in the distribution pattern of DDX6: the number of DDX6 granules per cell was reduced during adipogenesis and was enhanced during osteogenesis.

Human Amniotic Epithelial Cells as a Tool to Investigate the Effects of Cyanidin 3-O-Glucoside on Cell Differentiation

“…A downregulated gene, DDX6 , which encodes DEAD-box helicase 6 protein, has been reported to function in adipogenesis. In human adipose tissue-derived stem cells, the number of DDX6 granules per cell was reduced during adipogenesis [ 42 ].…”

3D Poly(Lactic Acid) Scaffolds Promote Different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparison With Standard 2D Cultures

“…hASCs were isolated from adipose tissue obtained from one otherwise healthy female donor (BMI = 27.03) who had undergone liposuction surgery. The isolation protocol followed previously published procedures ( Rebelatto et al, 2008 ; Marcon et al, 2020 ). In short, the tissue was first washed with sterile phosphate-buffered saline (PBS) and then digested with collagenase type I (Gibco ® , Invitrogen ® , Carlsbad, CA, United States) for 30 min at 37°C, 5% CO 2 and under constant agitation.…”

RNA Helicases as Shadow Modulators of Cell Cycle Progression

“…Inhibition of DDX3 causes a decrease in the amount of cyclin E1, A1 and CDK2 and stops the progress of cell cycle [ 39 , 52 , 53 , 147 ]. A decrease in DDX46 affects cell recovery from DNA-damage-dependent G1/S cell cycle arrest [ 57 ]. DDX21 is important for the expression of cyclin D1 and activation of C1 protein kinase.…”

“…Helicase DDX46 is involved in pre-mRNA splicing [ 55 ] and innate antiviral response by recruiting the m6A “eraser” ALKBH5 [ 56 ]. In addition, inhibition of DDX46 causes cell arrest in G1 phase and apoptosis via phosphorylation of Akt1 protein kinase and IkBa inhibitor [ 56 , 57 ]. Thus, the effect of DDX46 inhibitors may result from the combined effect of PI3K/Akt downregulation and changes in the cell cycle.…”